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Title: Crumpling of silver nanowires by endolysosomes strongly reduces toxicity

Abstract

Fibrous particles interact with cells and organisms in complex ways that can lead to cellular dysfunction, cell death, inflammation, and disease. The development of conductive transparent networks (CTNs) composed of metallic silver nanowires (AgNWs) for flexible touchscreen displays raises new possibilities for the intimate contact between novel fibers and human skin. Here, we report that a material property, nanowire-bending stiffness that is a function of diameter, controls the cytotoxicity of AgNWs to nonimmune cells from humans, mice, and fish without deterioration of critical CTN performance parameters: electrical conductivity and optical transparency. Both 30- and 90-nm-diameter AgNWs are readily internalized by cells, but thinner NWs are mechanically crumpled by the forces imposed during or after endocytosis, while thicker nanowires puncture the enclosing membrane and release silver ions and lysosomal contents to the cytoplasm, thereby initiating oxidative stress. This finding extends the fiber pathology paradigm and will enable the manufacture of safer products incorporating AgNWs.

Authors:
 [1];  [1];  [2];  [3];  [3];  [1];  [1]; ORCiD logo [2]; ORCiD logo [2]; ORCiD logo [4];  [2];  [2]; ORCiD logo [5];  [5];  [3];  [1];  [1];  [1];  [6]
  1. Univ. of Grenoble (France)
  2. European Synchrotron Radiation Facility (ESRF), Grenoble (France)
  3. Univ. of Florida, Gainesville, FL (United States)
  4. European Synchrotron Radiation Facility (ESRF), Grenoble (France); National Institute of Health and Medical Research (INSERM), Grenoble (France)
  5. Univ. of Lille (France); Univ. of the Littoral Opal Coast, Wimereux (France)
  6. Univ. of Grenoble (France); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
OSTI Identifier:
1564566
Alternate Identifier(s):
OSTI ID: 1559232
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Published Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 116; Journal Issue: 30; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; nanotechnology; endocytosis; fiber toxicity

Citation Formats

Lehmann, Sylvia G., Toybou, Djadidi, Pradas del Real, Ana-Elena, Arndt, Devrah, Tagmount, Abderrahmane, Viau, Muriel, Safi, Malak, Pacureanu, Alexandra, Cloetens, Peter, Bohic, Sylvain, Salomé, Murielle, Castillo-Michel, Hiram, Omaña-Sanz, Brenda, Hofmann, Annette, Vulpe, Christopher, Simonato, Jean-Pierre, Celle, Caroline, Charlet, Laurent, and Gilbert, Benjamin. Crumpling of silver nanowires by endolysosomes strongly reduces toxicity. United States: N. p., 2019. Web. doi:10.1073/pnas.1820041116.
Lehmann, Sylvia G., Toybou, Djadidi, Pradas del Real, Ana-Elena, Arndt, Devrah, Tagmount, Abderrahmane, Viau, Muriel, Safi, Malak, Pacureanu, Alexandra, Cloetens, Peter, Bohic, Sylvain, Salomé, Murielle, Castillo-Michel, Hiram, Omaña-Sanz, Brenda, Hofmann, Annette, Vulpe, Christopher, Simonato, Jean-Pierre, Celle, Caroline, Charlet, Laurent, & Gilbert, Benjamin. Crumpling of silver nanowires by endolysosomes strongly reduces toxicity. United States. doi:10.1073/pnas.1820041116.
Lehmann, Sylvia G., Toybou, Djadidi, Pradas del Real, Ana-Elena, Arndt, Devrah, Tagmount, Abderrahmane, Viau, Muriel, Safi, Malak, Pacureanu, Alexandra, Cloetens, Peter, Bohic, Sylvain, Salomé, Murielle, Castillo-Michel, Hiram, Omaña-Sanz, Brenda, Hofmann, Annette, Vulpe, Christopher, Simonato, Jean-Pierre, Celle, Caroline, Charlet, Laurent, and Gilbert, Benjamin. Mon . "Crumpling of silver nanowires by endolysosomes strongly reduces toxicity". United States. doi:10.1073/pnas.1820041116.
@article{osti_1564566,
title = {Crumpling of silver nanowires by endolysosomes strongly reduces toxicity},
author = {Lehmann, Sylvia G. and Toybou, Djadidi and Pradas del Real, Ana-Elena and Arndt, Devrah and Tagmount, Abderrahmane and Viau, Muriel and Safi, Malak and Pacureanu, Alexandra and Cloetens, Peter and Bohic, Sylvain and Salomé, Murielle and Castillo-Michel, Hiram and Omaña-Sanz, Brenda and Hofmann, Annette and Vulpe, Christopher and Simonato, Jean-Pierre and Celle, Caroline and Charlet, Laurent and Gilbert, Benjamin},
abstractNote = {Fibrous particles interact with cells and organisms in complex ways that can lead to cellular dysfunction, cell death, inflammation, and disease. The development of conductive transparent networks (CTNs) composed of metallic silver nanowires (AgNWs) for flexible touchscreen displays raises new possibilities for the intimate contact between novel fibers and human skin. Here, we report that a material property, nanowire-bending stiffness that is a function of diameter, controls the cytotoxicity of AgNWs to nonimmune cells from humans, mice, and fish without deterioration of critical CTN performance parameters: electrical conductivity and optical transparency. Both 30- and 90-nm-diameter AgNWs are readily internalized by cells, but thinner NWs are mechanically crumpled by the forces imposed during or after endocytosis, while thicker nanowires puncture the enclosing membrane and release silver ions and lysosomal contents to the cytoplasm, thereby initiating oxidative stress. This finding extends the fiber pathology paradigm and will enable the manufacture of safer products incorporating AgNWs.},
doi = {10.1073/pnas.1820041116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
issn = {0027-8424},
number = 30,
volume = 116,
place = {United States},
year = {2019},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1073/pnas.1820041116

Figures / Tables:

Fig. 1 Fig. 1: Diameter-dependent performance and cytotoxicity of AgNWs. (A) Scanning-electron microscopy images of 8- to 9-μm long AgNW with 3 mean diameters. (B) Relationship between optical transparency and sheet resistance for CTNs fabricated by ∼9-μm-long AgNWs with three different diameters. Each data point represents a spray-coated network at a differentmore » AgNW density. Yellow-shaded region represents the technological target for commercial CTNs. (C) Viability of murine fibroblasts cells following 24-h exposure with AgNWs of different diameter, as determined by the MTT assay. The dashed lines are model predictions as described in the text. (D) Viability of rainbow trout gut cells following 24-h exposure with AgNWs of different diameter determined by CellTiter-Glo assay. Cell-viability data are presented as a percentages relative to the nontreated control cells. Error bars on triplicate measurements are 1 SD.« less

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