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Title: 18. Combining experiment and simulation to study complex biomimetic membranes

Abstract

The three-dimensional architecture of biological membranes has functional consequences for the living cell. In the outer leaflet of the plasma membrane, lipids are thought to self-organize into domains enriched in high-melting lipids and cholesterol. Currently, there is much evidence implicating these lipid domains in a variety of membrane processes, including protein sorting, cell signaling, and the maintenance of membrane physical properties. Cells also actively maintain an asymmetric distribution of different lipid types between their plasma membrane inner and outer leaflets, resulting in monolayers with different fluidities and charge densities. Moreover, it is an open question how these and other bilayer properties are coupled, if at all. A variety of biophysical techniques are being employed to interrogate both lateral and transverse bilayer structures with sub-nanometer resolution. Molecular dynamic simulations are also used to probe the membrane’s finer details, providing a glimpse of membrane organization with atomic resolution. In this chapter, we present a discussion of these two approaches to investigate the organization of complex biomimetic membranes and present recent examples of how they have been used to complement each other.

Authors:
ORCiD logo [1];  [2]
  1. ORNL
  2. University of Cincinnati
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1561600
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Book
Country of Publication:
United States
Language:
English

Citation Formats

Katsaras, John, and Nickels, Jonathan. 18. Combining experiment and simulation to study complex biomimetic membranes. United States: N. p., 2019. Web.
Katsaras, John, & Nickels, Jonathan. 18. Combining experiment and simulation to study complex biomimetic membranes. United States.
Katsaras, John, and Nickels, Jonathan. Mon . "18. Combining experiment and simulation to study complex biomimetic membranes". United States.
@article{osti_1561600,
title = {18. Combining experiment and simulation to study complex biomimetic membranes},
author = {Katsaras, John and Nickels, Jonathan},
abstractNote = {The three-dimensional architecture of biological membranes has functional consequences for the living cell. In the outer leaflet of the plasma membrane, lipids are thought to self-organize into domains enriched in high-melting lipids and cholesterol. Currently, there is much evidence implicating these lipid domains in a variety of membrane processes, including protein sorting, cell signaling, and the maintenance of membrane physical properties. Cells also actively maintain an asymmetric distribution of different lipid types between their plasma membrane inner and outer leaflets, resulting in monolayers with different fluidities and charge densities. Moreover, it is an open question how these and other bilayer properties are coupled, if at all. A variety of biophysical techniques are being employed to interrogate both lateral and transverse bilayer structures with sub-nanometer resolution. Molecular dynamic simulations are also used to probe the membrane’s finer details, providing a glimpse of membrane organization with atomic resolution. In this chapter, we present a discussion of these two approaches to investigate the organization of complex biomimetic membranes and present recent examples of how they have been used to complement each other.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {7}
}

Book:
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