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Title: Determination of cis,cis- and cis,trans-Muconic Acid from Biological Conversion: Laboratory Analytical Procedure (LAP)

Abstract

Muconic acid is an important platform molecule for production of bio-based polymers. To date, cis,cis-muconic acid (ccMA) has been produced from multiple types of carbon sources including carbohydrates and aromatic compounds via cultivation with genetically engineered microbes. Accurate analysis of muconic acid has previously been a challenge due to ccMA isomerization into cis,trans-muconic acid (ctMA). This Laboratory Analytical Procedure (LAP) is used to separately quantify ccMA and ctMA as the total sum of muconic acid present in the liquid fraction of pretreated biological samples.

Authors:
 [1];  [1]; ORCiD logo [1]; ORCiD logo [1]
  1. National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1561511
Report Number(s):
NREL/TP-5100-74473
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; muconic acid; cis,cis-muconic acid; cis,trans-muconic acid; analytical procedure; HPLC; biological conversion; diode array detection (DAD); refractive index detection (RID); matrix effects; pretreated; M9 minimal media

Citation Formats

Pleitner, Brenna P, Michener, William E, Payne, Courtney E, and Beckham, Gregg T. Determination of cis,cis- and cis,trans-Muconic Acid from Biological Conversion: Laboratory Analytical Procedure (LAP). United States: N. p., 2019. Web. doi:10.2172/1561511.
Pleitner, Brenna P, Michener, William E, Payne, Courtney E, & Beckham, Gregg T. Determination of cis,cis- and cis,trans-Muconic Acid from Biological Conversion: Laboratory Analytical Procedure (LAP). United States. doi:10.2172/1561511.
Pleitner, Brenna P, Michener, William E, Payne, Courtney E, and Beckham, Gregg T. Fri . "Determination of cis,cis- and cis,trans-Muconic Acid from Biological Conversion: Laboratory Analytical Procedure (LAP)". United States. doi:10.2172/1561511. https://www.osti.gov/servlets/purl/1561511.
@article{osti_1561511,
title = {Determination of cis,cis- and cis,trans-Muconic Acid from Biological Conversion: Laboratory Analytical Procedure (LAP)},
author = {Pleitner, Brenna P and Michener, William E and Payne, Courtney E and Beckham, Gregg T},
abstractNote = {Muconic acid is an important platform molecule for production of bio-based polymers. To date, cis,cis-muconic acid (ccMA) has been produced from multiple types of carbon sources including carbohydrates and aromatic compounds via cultivation with genetically engineered microbes. Accurate analysis of muconic acid has previously been a challenge due to ccMA isomerization into cis,trans-muconic acid (ctMA). This Laboratory Analytical Procedure (LAP) is used to separately quantify ccMA and ctMA as the total sum of muconic acid present in the liquid fraction of pretreated biological samples.},
doi = {10.2172/1561511},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {9}
}

Technical Report:

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