Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome-dependent degradation of PAL

Kim, Jeong Im; Zhang, Xuebin; Pascuzzi, Pete; Liu, Chang-Jun; Chapple, Clint

doi:10.1111/nph.16108

Title: Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome-dependent degradation of PAL

Journal Article · Tue Aug 13 00:00:00 EDT 2019 · New Phytologist

DOI:https://doi.org/10.1111/nph.16108· OSTI ID:1597911

Kim, Jeong Im ^[1]; Zhang, Xuebin ^[2]; Pascuzzi, Pete ^[3]; Liu, Chang-Jun ^[4]; Chapple, Clint ^[3]

Purdue Univ., West Lafayette, IN (United States); Univ. of Florida, Gainesville, FL (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States); Henan Univ., Kaifeng (China)
Purdue Univ., West Lafayette, IN (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States)

Plants produce several hundreds of thousands of secondary metabolites that are important for adaptation to various environmental conditions. Although different groups of secondary metabolites are synthesized through unique biosynthetic pathways, plants must orchestrate their production simultaneously. Phenylpropanoids and glucosinolates are two classes of secondary metabolites that are synthesized through apparently independent biosynthetic pathways. Genetic evidence has revealed that the accumulation of glucosinolate intermediates limits phenylpropanoid production in a Mediator Subunit 5 (MED5)-dependent manner. To elucidate the molecular mechanism underlying this process, here we analyzed the transcriptomes of a suite of Arabidopsis thaliana glucosinolate-deficient mutants using RNAseq and identified misregulated genes that are rescued by the disruption of MED5. The expression of a group of Kelch Domain F-Box genes (KFBs) that function in PAL degradation is affected in glucosinolate biosynthesis mutants and the disruption of these KFBs restores phenylpropanoid deficiency in the mutants. Our study suggests that glucosinolate/phenylpropanoid metabolic crosstalk involves the transcriptional regulation of KFB genes that initiate the degradation of the enzyme phenylalanine ammonia-lyase, which catalyzes the first step of the phenylpropanoid biosynthesis pathway. Nevertheless, KFB mutant plants remain partially sensitive to glucosinolate pathway mutations, suggesting that other mechanisms that link the two pathways also exist.

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Research Organization:: Purdue Univ., West Lafayette, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-07ER15905; SC0012704; SC0000997

OSTI ID:: 1597911

Alternate ID(s):: OSTI ID: 1561330

Journal Information:: New Phytologist, Vol. 225, Issue 1; ISSN 0028-646X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 51 works

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59 BASIC BIOLOGICAL SCIENCES
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Title: Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome-dependent degradation of PAL

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