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Title: Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32

Abstract

The bioreduction of Fe(III) oxides by dissimilatory iron reducing bacteria (DIRB) may result in the production of a suite of Fe(II)-bearing secondary minerals, including magnetite, siderite, vivianite, green rusts, and chukanovite; the formation of specific phases controlled by the interaction of various physiological and geochemical factors. In an effort to better understand the effects of individual electron donors on the formation of specific Fe(II)-bearing secondary minerals, we examined the effects of a series of potential electron donors on the bioreduction of lepidocrocite ($$γ$$-FeOOH) by Shewanella putrefaciens CN32. Biomineralization products were identified by X-ray diffraction, Mössbauer spectroscopy, and scanning electron microscopy. Acetate, citrate, ethanol, glucose, glutamate, glycerol, malate, and succinate were not effectively utilized for the bioreduction of lepidocrocite by S. putrefaciens CN32; however, substantial Fe(II) production was observed when formate, lactate, H2, pyruvate, serine, or N acetylglucosamine (NAG) was provided as an electron donor. Carbonate or sulfate green rust was the dominant Fe(II)-bearing secondary mineral when formate, H2, lactate, or NAG was provided, however, siderite formed with pyruvate or serine. Geochemical modeling indicated that pH and carbonate concentration are the key factors determining the prevalence of carbonate green rust verses siderite.

Authors:
 [1]; ORCiD logo [2]; ORCiD logo [3];  [4]
  1. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Div.
  2. Univ. of Iowa, Iowa City, IA (United States). Dept. of Civil and Environmental Engineering; Pennsylvania State Univ., University Park, PA (United States). Dept. of Civil and Environmental Engineering
  3. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Div.; California Dept. of Water Resources, Sacramento, CA (United States)
  4. Univ. of Iowa, Iowa City, IA (United States). Dept. of Civil and Environmental Engineering
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science - Office of Biological and Environmental Research - Subsurface Biogeochemical Research
OSTI Identifier:
1560537
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Minerals
Additional Journal Information:
Journal Volume: 9; Journal Issue: 7; Journal ID: ISSN 2075-163X
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
58 GEOSCIENCES; dissimilatory iron reduction; green rust; iron oxide; siderite

Citation Formats

O’Loughlin, Edward J., Gorski, Christopher A., Flynn, Theodore M., and Scherer, Michelle M. Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32. United States: N. p., 2019. Web. doi:10.3390/min9070434.
O’Loughlin, Edward J., Gorski, Christopher A., Flynn, Theodore M., & Scherer, Michelle M. Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32. United States. https://doi.org/10.3390/min9070434
O’Loughlin, Edward J., Gorski, Christopher A., Flynn, Theodore M., and Scherer, Michelle M. 2019. "Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32". United States. https://doi.org/10.3390/min9070434. https://www.osti.gov/servlets/purl/1560537.
@article{osti_1560537,
title = {Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32},
author = {O’Loughlin, Edward J. and Gorski, Christopher A. and Flynn, Theodore M. and Scherer, Michelle M.},
abstractNote = {The bioreduction of Fe(III) oxides by dissimilatory iron reducing bacteria (DIRB) may result in the production of a suite of Fe(II)-bearing secondary minerals, including magnetite, siderite, vivianite, green rusts, and chukanovite; the formation of specific phases controlled by the interaction of various physiological and geochemical factors. In an effort to better understand the effects of individual electron donors on the formation of specific Fe(II)-bearing secondary minerals, we examined the effects of a series of potential electron donors on the bioreduction of lepidocrocite ($γ$-FeOOH) by Shewanella putrefaciens CN32. Biomineralization products were identified by X-ray diffraction, Mössbauer spectroscopy, and scanning electron microscopy. Acetate, citrate, ethanol, glucose, glutamate, glycerol, malate, and succinate were not effectively utilized for the bioreduction of lepidocrocite by S. putrefaciens CN32; however, substantial Fe(II) production was observed when formate, lactate, H2, pyruvate, serine, or N acetylglucosamine (NAG) was provided as an electron donor. Carbonate or sulfate green rust was the dominant Fe(II)-bearing secondary mineral when formate, H2, lactate, or NAG was provided, however, siderite formed with pyruvate or serine. Geochemical modeling indicated that pH and carbonate concentration are the key factors determining the prevalence of carbonate green rust verses siderite.},
doi = {10.3390/min9070434},
url = {https://www.osti.gov/biblio/1560537}, journal = {Minerals},
issn = {2075-163X},
number = 7,
volume = 9,
place = {United States},
year = {Sun Jul 14 00:00:00 EDT 2019},
month = {Sun Jul 14 00:00:00 EDT 2019}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 19 works
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Figures / Tables:

Figure 1 Figure 1: Fe(II) production during the bioreduction of lepidocrocite by S. putrefaciens CN32 as a function of available electron donor.

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Works referenced in this record:

Transformation of Hematite into Magnetite During Dissimilatory Iron Reduction—Conditions and Mechanisms
journal, August 2007


Use of Ferric and Ferrous Iron Containing Minerals for Respiration by Desulfitobacterium frappieri
journal, March 2003


Reduction of ferric green rust by Shewanella putrefaciens
journal, November 2007


Reductive Capacity of Natural Reductants
journal, February 2003


Isolation of Phyllosilicate–Iron Redox Cycling Microorganisms from an Illite–Smectite Rich Hydromorphic Soil
journal, January 2012


Ferrozine---a new spectrophotometric reagent for iron
journal, June 1970


Iron(II,III) Hydroxycarbonate Green Rust Formation and Stabilization from Lepidocrocite Bioreduction
journal, January 2002


Thermodynamic Equilibria in Aqueous Suspensions of Synthetic and Natural Fe(II)−Fe(III) Green Rusts:  Occurrences of the Mineral in Hydromorphic Soils
journal, April 1998


The impact of bacterial strain on the products of dissimilatory iron reduction
journal, January 2010


Secondary mineralization pathways induced by dissimilatory iron reduction of ferrihydrite under advective flow
journal, August 2003


Abiotic Transformation of Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by Green Rusts
journal, June 2008


Abiotic Nitrate Reduction to Ammonium:  Key Role of Green Rust
journal, January 1996


Bacterial reduction of crystalline Fe (super 3+) oxides in single phase suspensions and subsurface materials
journal, December 1998


Reduction kinetics of Fe(III), Co(III), U(VI), Cr(VI), and Tc(VII) in cultures of dissimilatory metal-reducing bacteria
journal, October 2002


Mineral transformations associated with the microbial reduction of magnetite
journal, September 2000


Occurrence and degradation of peptidoglycan in aquatic environments
journal, December 2003


Mössbauer and XAS study of a green rust mineral; the partial substitution of Fe 2+ by Mg 2+
journal, May 2001


Widespread N-Acetyl-D-Glucosamine Uptake among Pelagic Marine Bacteria and Its Ecological Implications
journal, November 2002


Microbial reduction of fe(III) in the Fithian and Muloorina illites: contrasting extents and rates of bioreduction
journal, February 2006


Contribution of Anionic vs. Neutral Polymers to the Formation of Green Rust 1 from γ-FeOOH Bioreduction
journal, August 2013


Impact of Organic Carbon Electron Donors on Microbial Community Development under Iron- and Sulfate-Reducing Conditions
journal, January 2016


Impact of Sediment-Bound Iron on Redox Buffering in a Landfill Leachate Polluted Aquifer (Vejen, Denmark)
journal, January 1995


Biomineralization of Poorly Crystalline Fe(III) Oxides by Dissimilatory Metal Reducing Bacteria (DMRB)
journal, March 2002


U(VI) Reduction by Biogenic and Abiotic Hydroxycarbonate Green Rusts: Impacts on U(IV) Speciation and Stability Over Time
journal, March 2018


Enriched Iron(III)-Reducing Bacterial Communities are Shaped by Carbon Substrate and Iron Oxide Mineralogy
journal, January 2012


Formation of Green Rust and Immobilization of Nickel in Response to Bacterial Reduction of Hydrous Ferric Oxide
journal, October 2001


Abiotic reduction of antimony(V) by green rust (Fe4(II)Fe2(III)(OH)12SO4·3H2O)
journal, January 2008


Nomenclature of the hydrotalcite supergroup: natural layered double hydroxides
journal, October 2012


Iron phase transformations resulting from the respiration of Shewanella putrefaciens on a mixed mineral phase
journal, November 2009


Kinetic Analysis of the Bacterial Reduction of Goethite
journal, June 2001


Constraint-Based Model of Shewanella oneidensis MR-1 Metabolism: A Tool for Data Analysis and Hypothesis Generation
journal, June 2010


Selenite Reduction by Mackinawite, Magnetite and Siderite: XAS Characterization of Nanosized Redox Products
journal, March 2008


Identification of green rust in an ochre sludge
journal, December 1991


Organic Acid-Dependent Iron Mineral Formation by a Newly Isolated Iron-Reducing Bacterium, Shewanella sp. HN-41
journal, January 2007


The formation of green rust induced by tropical river biofilm components
journal, June 2011


Metal reduction at cold temperatures by Shewanella isolates from various marine environments
journal, January 2005


Identification of a green rust mineral in a reductomorphic soil by Mossbauer and Raman spectroscopies
journal, March 1997


Uncoupling of chitinase activity and uptake of hydrolysis products in freshwater bacterioplankton
journal, May 2011


The reduction of chromate ions by Fe(II) layered hydroxides
journal, January 1999


Geobacter hydrogenophilus, Geobacter chapellei and Geobacter grbiciae, three new, strictly anaerobic, dissimilatory Fe(III)-reducers.
journal, March 2001


Dissolution and Reduction of Magnetite by Bacteria
journal, October 1995


Shewanella profunda sp. nov., isolated from deep marine sediment of the Nankai Trough
journal, November 2004


The anionic species competition in iron aqueous corrosion: Role of various green rust compounds
journal, September 1997


Biogenic iron mineralization accompanying the dissimilatory reduction of hydrous ferric oxide by a groundwater bacterium
journal, October 1998


Kinetic and Thermodynamic Analysis During Dissimilatory γ-FeOOH Reduction: Formation of Green Rust 1 and Magnetite
journal, January 2007


Effects of Bound Phosphate on the Bioreduction of Lepidocrocite (γ-FeOOH) and Maghemite (γ-Fe 2 O 3 ) and Formation of Secondary Minerals
journal, August 2013


Reduction of AgI, AuIII, CuII, and HgII by FeII/FeIII hydroxysulfate green rust
journal, November 2003


Respiration and Dissolution of Iron(III)-Containing Clay Minerals by Bacteria
journal, September 1999


Metal reduction and biomineralization by an alkaliphilic metal-reducing bacterium,Alkaliphilus metalliredigens (QYMF)
journal, December 2007


Effects of Oxyanions, Natural Organic Matter, and Bacterial Cell Numbers on the Bioreduction of Lepidocrocite (γ-FeOOH) and the Formation of Secondary Mineralization Products
journal, June 2010


Deferribacter thermophilus gen. nov., sp. nov., a Novel Thermophilic Manganese- and Iron-Reducing Bacterium Isolated from a Petroleum Reservoir
journal, April 1997


Reduction of Halogenated Ethanes by Green rust
journal, January 2004


Geovibrio ferrireducens , a phylogenetically distinct dissimilatory Fe(III)-reducing bacterium
journal, June 1996


Reduction of Aqueous Chromate by Fe(II)/Fe(III) Carbonate Green Rust:  Kinetic and Mechanistic Studies
journal, September 2004


Controls on Fe reduction and mineral formation by a subsurface bacterium
journal, April 2003


Formation of Hydroxysulphate Green Rust 2 as a Single Iron(II-III) Mineral in Microbial Culture
journal, October 2005


Effects of Electron Transfer Mediators on the Bioreduction of Lepidocrocite (γ-FeOOH) by Shewanella putrefaciens CN32
journal, September 2008


The reduction of aqueous Au 3+ by sulfide minerals and green rust phases
journal, May 2003


Enumeration and Characterization of Iron(III)-Reducing Microbial Communities from Acidic Subsurface Sediments Contaminated with Uranium(VI)
journal, December 2003


Kinetics and Structural Constraints of Chromate Reduction by Green Rusts
journal, June 2003


Anaerobic production of magnetite by a dissimilatory iron-reducing microorganism
journal, November 1987


Bacterial chitin degradation—mechanisms and ecophysiological strategies
journal, January 2013


Green Rust Formation from the Bioreduction of γ –FeOOH (Lepidocrocite): Comparison of Several Shewanella Species
journal, July 2007


Treatment of radioactive wastes: An X-ray absorption spectroscopy study of the reaction of technetium with green rust
journal, December 2003


XAFS Investigation of the Interactions of U VI with Secondary Mineralization Products from the Bioreduction of Fe III Oxides
journal, March 2010


Effects of Phosphate on Secondary Mineral Formation During the Bioreduction of Akaganeite (.β-FeOOH): Green Rust Versus Framboidal Magnetite
journal, July 2015


Ferrous hydroxy carbonate is a stable transformation product of biogenic magnetite
journal, February 2005


The Influence of Carbon Source on the Products of Dissimilatory Iron Reduction
journal, September 2009


Genomic encyclopedia of sugar utilization pathways in the Shewanella genus
journal, January 2010


Orenia metallireducens sp. nov. Strain Z6, a Novel Metal-Reducing Member of the Phylum Firmicutes from the Deep Subsurface
journal, August 2016


In situ Mössbauer spectroscopy: Evidence for green rust (fougerite) in a gleysol and its mineralogical transformations with time and depth
journal, September 2005


Characterization and Description of Anaeromyxobacter dehalogenans gen. nov., sp. nov., an Aryl-Halorespiring Facultative Anaerobic Myxobacterium
journal, February 2002


Reductive Dechlorination of Carbon Tetrachloride Using Iron(II) Iron(III) Hydroxide Sulfate (Green Rust)
journal, January 1999


Archaea catalyze iron-dependent anaerobic oxidation of methane
journal, October 2016


Pyruvate and Lactate Metabolism by Shewanella oneidensis MR-1 under Fermentation, Oxygen Limitation, and Fumarate Respiration Conditions
journal, September 2011


Abiotic Reductive Dechlorination of Chlorinated Ethylenes by Iron-Bearing Soil Minerals. 2. Green Rust
journal, December 2002


EXAFS Analysis of Arsenite Adsorption onto Two-Line Ferrihydrite, Hematite, Goethite, and Lepidocrocite
journal, December 2005


Enzymatic iron and uranium reduction by sulfate-reducing bacteria
journal, July 1993


Identification of Green Rust in Groundwater
journal, May 2009


Green rust formation controls nutrient availability in a ferruginous water column
journal, July 2012


Ecology and Biotechnology of the Genus Shewanella
journal, October 2007


Mineralogical and morphological constraints on the reduction of Fe(III) minerals by Geobacter sulfurreducens
journal, July 2009


Geothrix fermentans gen. nov., sp. nov., a novel Fe(III)-reducing bacterium from a hydrocarbon-contaminated aquifer
journal, October 1999


Reactivity of Fe(II)-Bearing Minerals toward Reductive Transformation of Organic Contaminants
journal, February 2004


The anaerobic degradation of organic matter in Danish coastal sediments: Iron reduction, manganese reduction, and sulfate reduction
journal, August 1993


Towards environmental systems biology of Shewanella
journal, July 2008


Reduction of SeO 4 2 - Anions and Anoxic Formation of Iron(II)−Iron(III) Hydroxy-Selenate Green Rust
journal, March 2000


Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas.
journal, January 2002


The mechanisms of reduction of hexavalent chromium by green rust sodium sulphate: Formation of Cr-goethite
journal, July 2006


X-ray absorption and photoelectron spectroscopy investigation of selenite reduction by FeII-bearing minerals
journal, December 2008


Fougerite, a new mineral of the pyroaurite-iowaite group: description and crystal structure
journal, June 2007


Bacterial reduction of crystalline Fe (super 3+) oxides in single phase suspensions and subsurface materials
journal, December 1998


Reduction of Uranium(VI) by Mixed Iron(II)/Iron(III) Hydroxide (Green Rust):  Formation of UO 2 Nanoparticles
journal, February 2003


Biotransformation of two-line silica-ferrihydrite by a dissimilatory Fe(III)-reducing bacterium: formation of carbonate green rust in the presence of phosphate
journal, July 2004


BIOGEOCHEMICAL AND ENVIRONMENTAL FACTORS IN Fe BIOMINERALIZATION: MAGNETITE AND SIDERITE FORMATION
journal, February 2003


Biotransformation of lepidocrocite in the presence of quinones and flavins
journal, August 2013


Fe(II)/Fe(III) ‘green rust’ developed within ochreous coal mine drainage sediment in South Wales, UK
journal, December 2006


Microbial and plant uptake of free amino sugars in grassland soils
journal, June 2012


Observations and assessment of iron oxide and green rust nanoparticles in metal-polluted mine drainage within a steep redox gradient
journal, January 2014


Microbial Fe(III) reduction in subsurface environments
journal, July 1997


Neptunyl (Np) interaction with green rust,
journal, March 2011


Iron and Manganese in Anaerobic Respiration: Environmental Significance, Physiology, and Regulation
journal, October 1994


Arsenic sequestration by sorption processes in high-iron sediments
journal, December 2007


Characterization of Predominant Reductants in an Anaerobic Leachate-Contaminated Aquifer by Nitroaromatic Probe Compounds
journal, January 1998


Kinetics of Cr(VI) Reduction by Carbonate Green Rust
journal, September 2001


Thermoterrabacterium ferrireducens gen. nov., sp. nov., a Thermophilic Anaerobic Dissimilatory Fe(III)-Reducing Bacterium from a Continental Hot Spring
journal, April 1997


Phosphate Imposed Limitations on Biological Reduction and Alteration of Ferrihydrite
journal, January 2007


Hydrogen and Formate Oxidation Coupled to Dissimilatory Reduction of Iron or Manganese by Alteromonas putrefaciens
journal, January 1989


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