Microbial and metabolic succession on common building materials under high humidity conditions

Lax, Simon; Cardona, Cesar; Zhao, Dan; Winton, Valerie J.; Goodney, Gabriel; Gao, Peng; Gottel, Neil; Hartmann, Erica M.; Henry, Chris; Thomas, Paul M.; Kelley, Scott T.; Stephens, Brent; Gilbert, Jack A.

doi:10.1038/s41467-019-09764-z

Title: Microbial and metabolic succession on common building materials under high humidity conditions

Journal Article · Tue Apr 16 00:00:00 EDT 2019 · Nature Communications

DOI:https://doi.org/10.1038/s41467-019-09764-z· OSTI ID:1559538

Lax, Simon ^[1];

^[2]; Zhao, Dan ^[3];

^[4];

^[5]; Gao, Peng ^[4]; Gottel, Neil ^[6]; Hartmann, Erica M. ^[4]; Henry, Chris ^[7];

^[4]; Kelley, Scott T. ^[5]; Stephens, Brent ^[3];

^[6]

Univ. of Chicago, IL (United States); Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Univ. of Chicago, IL (United States)
Illinois Inst. of Technology, Chicago, IL (United States)
Northwestern Univ., Evanston, IL (United States)
San Diego State Univ., CA (United States)
Univ. of California, San Diego, CA (United States)
Argonne National Lab. (ANL), Lemont, IL (United States)

Despite considerable efforts to characterize the microbial ecology of the built environment, the metabolic mechanisms underpinning microbial colonization and successional dynamics remain unclear, particularly at high moisture conditions. Here, we applied bacterial/viral particle counting, qPCR, amplicon sequencing of the genes encoding 16S and ITS rRNA, and metabolomics to longitudinally characterize the ecological dynamics of four common building materials maintained at high humidity. We varied the natural inoculum provided to each material and wet half of the samples to simulate a potable water leak. Wetted materials had higher growth rates and lower alpha diversity compared to non-wetted materials, and wetting described the majority of the variance in bacterial, fungal, and metabolite structure. Inoculation location was weakly associated with bacterial and fungal beta diversity. Material type influenced bacterial and viral particle abundance and bacterial and metabolic (but not fungal) diversity. Metabolites indicative of microbial activity were identified, and they too differed by material.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: Alfred P. Sloan Foundation; USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1559538

Journal Information:: Nature Communications, Vol. 10, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 29 works

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Microbial and Metabolic Succession on Common Building Materials Under High Humidity Conditions Lax, Simon figshare https://doi.org/10.6084/m9.figshare.7865015.v2	dataset	January 2019

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