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Title: In vitro single-cell dissection revealing the interior structure of cable bacteria

Abstract

Filamentous Desulfobulbaceae bacteria were recently discovered as long-range transporters of electrons from sulphide to oxygen in marine sediments. The long-range electron transfer through these “cable bacteria” has created considerable interests, but it has also raised many questions, such as what structural basis will be required to enable micrometer-sized cells to build into centimeter-long continuous filaments? Here we dissected cable bacteria cells in vitro by atomic force microscopy and further explored the interior, which is normally hidden behind the outer membrane (OM). Using nanoscale topographical and mechanical maps, different types of bacterial cell-cell junctions and strings along the cable length were identified. More importantly: these strings were found to be continuous along the bacterial cells passing through the cell-cell junctions. This indicates that the strings serve an important function in maintaining integrity of individual cable bacteria cells as a united filament. Furthermore, ridges in the OM are found to envelop the individual strings at cell-cell junctions, and they are proposed to strengthen the cell-cell junctions. Finally, we propose a model for the division and growth of the cable bacteria, which illustrate the possible structural requirements for the formation of centimeter length filaments in the recently discovered cable bacteria.

Authors:
 [1]; ORCiD logo [2];  [1];  [1];  [1];  [1];  [3];  [4];  [5];  [1];  [1]
  1. Aarhus Universitet
  2. BATTELLE (PACIFIC NW LAB)
  3. Norwegian University of Science and Technology
  4. Harbin Institute of Technology
  5. University of Aarhus
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1558645
Report Number(s):
PNNL-SA-136014
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 115; Journal Issue: 34
Country of Publication:
United States
Language:
English

Citation Formats

Jiang, Zaixing, Zhang, Shuai, Hyldgaard Klausen, Lasse, Song, Jie, Li, Qiang, Wang, Zegao, Torger Stokke, Bjorn, Huang, Yudong, Besenbacher, Fleming, Nielsen, Lars P., and Dong, Mingdong. In vitro single-cell dissection revealing the interior structure of cable bacteria. United States: N. p., 2018. Web. doi:10.1073/pnas.1807562115.
Jiang, Zaixing, Zhang, Shuai, Hyldgaard Klausen, Lasse, Song, Jie, Li, Qiang, Wang, Zegao, Torger Stokke, Bjorn, Huang, Yudong, Besenbacher, Fleming, Nielsen, Lars P., & Dong, Mingdong. In vitro single-cell dissection revealing the interior structure of cable bacteria. United States. doi:10.1073/pnas.1807562115.
Jiang, Zaixing, Zhang, Shuai, Hyldgaard Klausen, Lasse, Song, Jie, Li, Qiang, Wang, Zegao, Torger Stokke, Bjorn, Huang, Yudong, Besenbacher, Fleming, Nielsen, Lars P., and Dong, Mingdong. Tue . "In vitro single-cell dissection revealing the interior structure of cable bacteria". United States. doi:10.1073/pnas.1807562115.
@article{osti_1558645,
title = {In vitro single-cell dissection revealing the interior structure of cable bacteria},
author = {Jiang, Zaixing and Zhang, Shuai and Hyldgaard Klausen, Lasse and Song, Jie and Li, Qiang and Wang, Zegao and Torger Stokke, Bjorn and Huang, Yudong and Besenbacher, Fleming and Nielsen, Lars P. and Dong, Mingdong},
abstractNote = {Filamentous Desulfobulbaceae bacteria were recently discovered as long-range transporters of electrons from sulphide to oxygen in marine sediments. The long-range electron transfer through these “cable bacteria” has created considerable interests, but it has also raised many questions, such as what structural basis will be required to enable micrometer-sized cells to build into centimeter-long continuous filaments? Here we dissected cable bacteria cells in vitro by atomic force microscopy and further explored the interior, which is normally hidden behind the outer membrane (OM). Using nanoscale topographical and mechanical maps, different types of bacterial cell-cell junctions and strings along the cable length were identified. More importantly: these strings were found to be continuous along the bacterial cells passing through the cell-cell junctions. This indicates that the strings serve an important function in maintaining integrity of individual cable bacteria cells as a united filament. Furthermore, ridges in the OM are found to envelop the individual strings at cell-cell junctions, and they are proposed to strengthen the cell-cell junctions. Finally, we propose a model for the division and growth of the cable bacteria, which illustrate the possible structural requirements for the formation of centimeter length filaments in the recently discovered cable bacteria.},
doi = {10.1073/pnas.1807562115},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 34,
volume = 115,
place = {United States},
year = {2018},
month = {8}
}

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