Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance

Jia, Huixia; Zhang, Jin; Li, Jianbo; Sun, Pei; Zhang, Yahong; Xin, Xuebing; Lu, Mengzhu; Hu, Jianjun

doi:10.1186/s12870-019-1900-1

Title: Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance

Journal Article · Thu Aug 15 00:00:00 EDT 2019 · BMC Plant Biology

DOI:https://doi.org/10.1186/s12870-019-1900-1· OSTI ID:1557471

Jia, Huixia ^[1];

^[2]; Li, Jianbo ^[1]; Sun, Pei ^[1]; Zhang, Yahong ^[1]; Xin, Xuebing ^[1]; Lu, Mengzhu ^[1]; Hu, Jianjun ^[1]

Chinese Academy of Forestry, Beijing (China)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Chinese Academy of Forestry, Beijing (China)

Background: Drought is a major environmental constraint to plant growth, development and productivity. Compared with most willows that are generally susceptible to drought, the desert willow Salix psammophila has extraordinary adaptation to drought stress. However, its molecular basis of drought tolerance is still largely unknown.Results: During polyethylene glycol 6000 (PEG 6000)-simulated drought stress, we found that the osmotic adjustment substances were accumulated and the antioxidant enzyme activities were enhanced in S. psammophila roots. A total of 8172 differentially expressed genes were identified in roots of S. psammophila through RNA-Sequencing. Based on K-means clustering, their expression patterns were classified into nine clusters, which were enriched in several stress-related processes including transcriptional regulation, response to various stresses, cell death, etc. Moreover, 672 transcription factors from 45 gene families were differentially expressed under drought stress. Furthermore, a weighted gene co-expression network was constructed, and eight genes were identified as hub genes. We demonstrated the function of two hub genes, magnesium-dependent phosphatase 1 (SpMDP1) and SpWRKY33, through overexpression in Arabidopsis thaliana. Overexpression of the two hub genes enhanced the drought tolerance in transgenic plants, suggesting that the identification of candidate drought tolerance genes in this study was highly efficient and credible.Conclusions: Our study analyzed the physiological and molecular responses to drought stress in S. psammophila, and these results contribute to dissect the mechanism of drought tolerance of S. psammophila and facilitate identification of critical genes involved in drought tolerance for willow breeding.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1557471

Journal Information:: BMC Plant Biology, Vol. 19, Issue 1; ISSN 1471-2229

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 7 works

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Additional file 1: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637604 The current record is supplemented by this dataset	dataset	January 2019
Additional file 9: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637628.v1 The current record is supplemented by this dataset	dataset	January 2019
Additional file 2: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637607 The current record is supplemented by this text	text	January 2019
Additional file 3: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637610 The current record is supplemented by this dataset	dataset	January 2019
Additional file 5: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637616 The current record is supplemented by this dataset	dataset	January 2019
Additional file 4: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637613 The current record is supplemented by this dataset	dataset	January 2019
Additional file 6: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637619 The current record is supplemented by this dataset	dataset	January 2019
Additional file 7: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637622 The current record is supplemented by this dataset	dataset	January 2019
Additional file 5: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637616.v1 The current record is supplemented by this dataset	dataset	January 2019
Additional file 7: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637622.v1 The current record is supplemented by this dataset	dataset	January 2019
Additional file 8: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637625 The current record is supplemented by this dataset	dataset	January 2019
Additional file 9: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637628 The current record is supplemented by this dataset	dataset	January 2019
Additional file 2: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637607.v1 The current record is supplemented by this text	text	January 2019
Additional file 4: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637613.v1 The current record is supplemented by this dataset	dataset	January 2019
Additional file 6: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637619.v1 The current record is supplemented by this dataset	dataset	January 2019
Additional file 8: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637625.v1 The current record is supplemented by this dataset	dataset	January 2019
Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.c.4619972.v1 The current record is supplemented by this collection	collection	January 2019
Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.c.4619972 The current record is supplemented by this collection	collection	January 2019
Additional file 3: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637610.v1 The current record is supplemented by this dataset	dataset	January 2019
Additional file 1: of Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance Jia, Huixia; Zhang, Jin; Li, Jianbo https://doi.org/10.6084/m9.figshare.9637604.v1 The current record is supplemented by this dataset	dataset	January 2019

Title: Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance

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