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Title: SCPAssistant v1.20

Abstract

Critical need to rapidly and quantitatively measure the chemistry of single cells in their natural state, without extensive sample preparation procedures. Developed at ORNL an innovative single cell droplet ejection-liquid vortex capture mass spectrometry (SCDE-LVC-MS) platform for rapid, in situ molecular characterization of single cells in suspension. This platform provides rapid, quantitative, targeted and/or untargeted mass spectrometric analysis of single cells without extensive sample preparation procedures. Enable real-time untargeted mass spectrometry analysis of chemical constituents of single cells in their native environment. Send a TTL signal via a connected AD/DA board to start mass spectrometry (MS) data acquisition - analyze MS data - classification of analyzed samples (e.g. separate single cell types based on their chemistry) - quantitation of analyzed samples (e.g. quantify lipids in single cells). List of features: external instrument control; screen capture; reporting; real-time MS data (single cell) analysis; classification; quantitation. Specific application: real-time mass spectrometry analysis of chemical constituents of single cells in their native environment. The software starts MS acquisition; then monitors a folder where the Cytena Single Cell Printer saves images of ejected single cells; real-time extracts MS spectra from MS files for a) either classification of single cells, or b) quantitation of (a)more » chemical constituent(s) of single cells.« less

Authors:
 [1];  [1]
  1. Oak Ridge National Laboratory
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1557122
Report Number(s):
SCPAssistant v1.20; 005871IBMPC00
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Software
Software Revision:
00
Software Package Number:
005871
Software CPU:
IBMPC
Source Code Available:
Yes
Country of Publication:
United States

Citation Formats

Kertesz, Vilmos, and Cahill, John F. SCPAssistant v1.20. Computer software. Vers. 00. USDOE. 29 Oct. 2018. Web.
Kertesz, Vilmos, & Cahill, John F. (2018, October 29). SCPAssistant v1.20 (Version 00) [Computer software].
Kertesz, Vilmos, and Cahill, John F. SCPAssistant v1.20. Computer software. Version 00. October 29, 2018.
@misc{osti_1557122,
title = {SCPAssistant v1.20, Version 00},
author = {Kertesz, Vilmos and Cahill, John F},
abstractNote = {Critical need to rapidly and quantitatively measure the chemistry of single cells in their natural state, without extensive sample preparation procedures. Developed at ORNL an innovative single cell droplet ejection-liquid vortex capture mass spectrometry (SCDE-LVC-MS) platform for rapid, in situ molecular characterization of single cells in suspension. This platform provides rapid, quantitative, targeted and/or untargeted mass spectrometric analysis of single cells without extensive sample preparation procedures. Enable real-time untargeted mass spectrometry analysis of chemical constituents of single cells in their native environment. Send a TTL signal via a connected AD/DA board to start mass spectrometry (MS) data acquisition - analyze MS data - classification of analyzed samples (e.g. separate single cell types based on their chemistry) - quantitation of analyzed samples (e.g. quantify lipids in single cells). List of features: external instrument control; screen capture; reporting; real-time MS data (single cell) analysis; classification; quantitation. Specific application: real-time mass spectrometry analysis of chemical constituents of single cells in their native environment. The software starts MS acquisition; then monitors a folder where the Cytena Single Cell Printer saves images of ejected single cells; real-time extracts MS spectra from MS files for a) either classification of single cells, or b) quantitation of (a) chemical constituent(s) of single cells.},
doi = {},
year = {2018},
month = {10},
note =
}

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