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Title: Involvement of CesA4, CesA7-A/B and CesA8-A/B in Secondary Wall Formation in Populus trichocarpa Wood

Abstract

Cellulose synthase A genes (CesAs) are responsible for cellulose biosynthesis in plant cell walls. In this study, functions of secondary wall cellulose synthases PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B were characterized during wood formation in Populus trichocarpa (Torr. & Gray). CesA RNAi knockdown transgenic plants exhibited stunted growth, narrow leaves, early necrosis, reduced stature, collapsed vessels, thinner fiber cell walls and extended fiber lumen diameters. In the RNAi knockdown transgenics, stems exhibited reduced mechanical strength, with reduced modulus of rupture (MOR) and modulus of elasticity (MOE). The reduced mechanical strength may be due to thinner fiber cell walls. Vessels in the xylem of the transgenics were collapsed, indicating that water transport in xylem may be affected and thus causing early necrosis in leaves. A dramatic decrease in cellulose content was observed in the RNAi knockdown transgenics. Compared with wildtype, the cellulose content was significantly decreased in the PtrCesA4, PtrCesA7 and PtrCesA8 RNAi knockdown transgenics. As a result, lignin and xylem contents were proportionally increased. The wood composition changes were confirmed by solid-state NMR, two-dimensional solution-state NMR and sum-frequency-generation vibration (SFG) analyses. Both solid-state nuclear magnetic resonance (NMR) and SFG analyses demonstrated that knockdown of PtrCesAs did not affect cellulose crystallinity index. Ourmore » results provided the evidence for the involvement of PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B in secondary cell wall formation in wood and demonstrated the pleiotropic effects of their perturbations on wood formation.« less

Authors:
 [1];  [2];  [2];  [3];  [4];  [5];  [6];  [7];  [8];  [5];  [2];  [7];  [7];  [2];  [9];  [5]; ORCiD logo [4];  [3];  [2];  [10] more »;  [7] « less
  1. Beijing Forestry University; Chinese Academy of Forestry
  2. North Carolina State University
  3. University of Wisconsin
  4. National Renewable Energy Laboratory (NREL), Golden, CO (United States)
  5. Pennsylvania State University
  6. South China University of Technology
  7. Chinese Academy of Forestry
  8. Guangxi University
  9. Beijing Forestry University
  10. North Carolina State University; Northeast Forestry University
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1557080
Report Number(s):
NREL/JA-2700-74549
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article
Journal Name:
Tree Physiology
Additional Journal Information:
Journal Name: Tree Physiology
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; cellulose; Populus trichocarpa; RNAi; secondary cell wall; wood formation

Citation Formats

Abbas, Manzar, Peszlen, Ilona, Shi, Rui, Kim, Hoon, Katahira, Rui, Kafle, Kabindra, Xiang, Zhouyang, Huang, Xiong, Min, Douyong, Mohamadamin, Makarem, Yang, Chenmin, Dai, Xinren, Yan, Xiaojing, Park, Sunkyu, Li, Yun, Kim, Seong H., Davis, Mark F, Ralph, John, Sederoff, Ronald R., Chiang, Vincent L., and Li, Quanzi. Involvement of CesA4, CesA7-A/B and CesA8-A/B in Secondary Wall Formation in Populus trichocarpa Wood. United States: N. p., 2019. Web. doi:10.1093/treephys/tpz020.
Abbas, Manzar, Peszlen, Ilona, Shi, Rui, Kim, Hoon, Katahira, Rui, Kafle, Kabindra, Xiang, Zhouyang, Huang, Xiong, Min, Douyong, Mohamadamin, Makarem, Yang, Chenmin, Dai, Xinren, Yan, Xiaojing, Park, Sunkyu, Li, Yun, Kim, Seong H., Davis, Mark F, Ralph, John, Sederoff, Ronald R., Chiang, Vincent L., & Li, Quanzi. Involvement of CesA4, CesA7-A/B and CesA8-A/B in Secondary Wall Formation in Populus trichocarpa Wood. United States. doi:10.1093/treephys/tpz020.
Abbas, Manzar, Peszlen, Ilona, Shi, Rui, Kim, Hoon, Katahira, Rui, Kafle, Kabindra, Xiang, Zhouyang, Huang, Xiong, Min, Douyong, Mohamadamin, Makarem, Yang, Chenmin, Dai, Xinren, Yan, Xiaojing, Park, Sunkyu, Li, Yun, Kim, Seong H., Davis, Mark F, Ralph, John, Sederoff, Ronald R., Chiang, Vincent L., and Li, Quanzi. Tue . "Involvement of CesA4, CesA7-A/B and CesA8-A/B in Secondary Wall Formation in Populus trichocarpa Wood". United States. doi:10.1093/treephys/tpz020.
@article{osti_1557080,
title = {Involvement of CesA4, CesA7-A/B and CesA8-A/B in Secondary Wall Formation in Populus trichocarpa Wood},
author = {Abbas, Manzar and Peszlen, Ilona and Shi, Rui and Kim, Hoon and Katahira, Rui and Kafle, Kabindra and Xiang, Zhouyang and Huang, Xiong and Min, Douyong and Mohamadamin, Makarem and Yang, Chenmin and Dai, Xinren and Yan, Xiaojing and Park, Sunkyu and Li, Yun and Kim, Seong H. and Davis, Mark F and Ralph, John and Sederoff, Ronald R. and Chiang, Vincent L. and Li, Quanzi},
abstractNote = {Cellulose synthase A genes (CesAs) are responsible for cellulose biosynthesis in plant cell walls. In this study, functions of secondary wall cellulose synthases PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B were characterized during wood formation in Populus trichocarpa (Torr. & Gray). CesA RNAi knockdown transgenic plants exhibited stunted growth, narrow leaves, early necrosis, reduced stature, collapsed vessels, thinner fiber cell walls and extended fiber lumen diameters. In the RNAi knockdown transgenics, stems exhibited reduced mechanical strength, with reduced modulus of rupture (MOR) and modulus of elasticity (MOE). The reduced mechanical strength may be due to thinner fiber cell walls. Vessels in the xylem of the transgenics were collapsed, indicating that water transport in xylem may be affected and thus causing early necrosis in leaves. A dramatic decrease in cellulose content was observed in the RNAi knockdown transgenics. Compared with wildtype, the cellulose content was significantly decreased in the PtrCesA4, PtrCesA7 and PtrCesA8 RNAi knockdown transgenics. As a result, lignin and xylem contents were proportionally increased. The wood composition changes were confirmed by solid-state NMR, two-dimensional solution-state NMR and sum-frequency-generation vibration (SFG) analyses. Both solid-state nuclear magnetic resonance (NMR) and SFG analyses demonstrated that knockdown of PtrCesAs did not affect cellulose crystallinity index. Our results provided the evidence for the involvement of PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B in secondary cell wall formation in wood and demonstrated the pleiotropic effects of their perturbations on wood formation.},
doi = {10.1093/treephys/tpz020},
journal = {Tree Physiology},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {6}
}

Works referenced in this record:

Solution-state 2D NMR of Ball-milled Plant Cell Wall Gels in DMSO-d 6
journal, March 2008


Genetic Transformation of Populus trichocarpa Genotype Nisqually-1: A Functional Genomic Tool for Woody Plants
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Collapsed Xylem Phenotype of Arabidopsis Identifies Mutants Deficient in Cellulose Deposition in the Secondary Cell Wall
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The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray)
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A xylem-specific cellulose synthase gene from aspen (Populus tremuloides) is responsive to mechanical stress
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