Diversion of Carbon Flux from Sugars to Lipids Improves the Growth of an Arabidopsis Starchless Mutant

Fan, Jilian; Zhou, Chao; Yu, Linhui; Li, Ping; Shanklin, John; Xu, Changcheng

doi:10.3390/plants8070229

Title: Diversion of Carbon Flux from Sugars to Lipids Improves the Growth of an Arabidopsis Starchless Mutant

Journal Article · Wed Jul 17 00:00:00 EDT 2019 · Plants

DOI:https://doi.org/10.3390/plants8070229· OSTI ID:1543017

Fan, Jilian;

;

; Li, Ping; Shanklin, John;

Inactivation of ADP-glucose pyrophosphorylase1 (ADG1) causes a starchless phenotype in Arabidopsis. Mutants defective in ADG1 show severe growth retardation in day/night conditions but exhibit similar growth to the wild type under continuous light, implying an important role of starch in supporting respiration, metabolism and growth at night. In addition to carbohydrates, lipids and proteins can serve as alternative respiratory substrates for energy production in mature plants. To test the role of lipids in diurnal growth, we generated transgenic plants overexpressing phospholipid:diacylglycerol acyltransferase1 (PDAT1) in adg1. We found that PDAT1 overexpression caused an increase in both fatty acid synthesis and turnover and increased accumulation of triacylglycerol (TAG) at the expense of sugars, and improved the diurnal growth of adg1. We demonstrated that unlike sugars, which were metabolized within a few hours of darkness, TAG breakdown was slow, occurring throughout the entire dark period. The slow pace of TAG hydrolysis enabled a sustained supply of fatty acids for energy production, thereby alleviating energy deficiency at night and hence improving growth of the starchless mutants. We conclude that lipids can contribute to diurnal plant growth by providing a constant supply of fatty acids as an alternative energy source in mature starchless mutant plants.

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Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division

Grant/Contract Number:: SC0018420 and DE-SC0012704; SC0018420; SC0012704

OSTI ID:: 1543017

Alternate ID(s):: OSTI ID: 1546074; OSTI ID: 1556883

Report Number(s):: BNL-211945-2019-JAAM; PLANCD; PII: plants8070229

Journal Information:: Plants, Journal Name: Plants Vol. 8 Journal Issue: 7; ISSN 2223-7747

Publisher:: MDPI AGCopyright Statement

Country of Publication:: Netherlands

Language:: English

Citation Metrics:

Cited by: 10 works

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References (34)

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Title: Diversion of Carbon Flux from Sugars to Lipids Improves the Growth of an Arabidopsis Starchless Mutant

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