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Title: FDX5 Deletion Affects Metabolism of Algae During the Different Phases of S-Deprivation

Abstract

Ferredoxin5 (FDX5), a minor ferredoxin protein in the alga, Chlamydomonas (Chlamydomonas reinhardtii), helps maintain thylakoid membrane integrity in the dark. Sulfur (S) deprivation has been used to achieve prolonged hydrogen production in green algae. Here, we propose that FDX5 is involved in algal responses to sulfur-deprivation as well as to the dark. Specifically, we tested the role of FDX5 in both the initial aerobic and subsequent anaerobic phases of S-deprivation. Under S-deprived conditions, absence of FDX5 causes a distinct delay in achieving anoxia by affecting photosynthetic O2 evolution, accompanied by reduced acetate uptake, lower starch accumulation, and delayed/lower fermentative metabolite production, including photohydrogen. We attribute these differences to transcriptional and/or post-translational regulation of acetyl-CoA synthetase and ADP-glucose pyrophosphorylase, and increased stability of the Photosystem II D1 protein. Interestingly, increased levels of Ferredoxin2 and Ferredoxin1 were observed in the mutant under oxic, S-replete conditions, strengthening our previously proposed hypothesis that other ferredoxins compensate in response to a lack of FDX5. Taken together, the results of our omics and pull-down experiments confirmed biochemical and physiological results, suggesting that FDX5 may have other effects on Chlamydomonas metabolism through its interaction with multiple redox partners.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [3];  [1]
  1. National Renewable Energy Laboratory (NREL), Golden, CO (United States)
  2. University of Cordoba, Spain
  3. University Federal de Santa Catarina
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1550783
Report Number(s):
NREL/JA-2700-73314
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article
Journal Name:
Plant Physiology
Additional Journal Information:
Journal Name: Plant Physiology
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; omics; ferredoxin5; Chlamydomonas reinhardtii; sulfur deprivation; pathways

Citation Formats

Subramanian, Venkataramanan, Wecker, Matt, Gerritsen, Alida, Boehm, Marko, Xiong, Wei, wachter, benton, Dubini, Alexandra, Gonzalez-Ballester, David, Antonio, Regina V., and Ghirardi, Maria L. FDX5 Deletion Affects Metabolism of Algae During the Different Phases of S-Deprivation. United States: N. p., 2019. Web. doi:10.1104/pp.19.00457.
Subramanian, Venkataramanan, Wecker, Matt, Gerritsen, Alida, Boehm, Marko, Xiong, Wei, wachter, benton, Dubini, Alexandra, Gonzalez-Ballester, David, Antonio, Regina V., & Ghirardi, Maria L. FDX5 Deletion Affects Metabolism of Algae During the Different Phases of S-Deprivation. United States. doi:10.1104/pp.19.00457.
Subramanian, Venkataramanan, Wecker, Matt, Gerritsen, Alida, Boehm, Marko, Xiong, Wei, wachter, benton, Dubini, Alexandra, Gonzalez-Ballester, David, Antonio, Regina V., and Ghirardi, Maria L. Fri . "FDX5 Deletion Affects Metabolism of Algae During the Different Phases of S-Deprivation". United States. doi:10.1104/pp.19.00457.
@article{osti_1550783,
title = {FDX5 Deletion Affects Metabolism of Algae During the Different Phases of S-Deprivation},
author = {Subramanian, Venkataramanan and Wecker, Matt and Gerritsen, Alida and Boehm, Marko and Xiong, Wei and wachter, benton and Dubini, Alexandra and Gonzalez-Ballester, David and Antonio, Regina V. and Ghirardi, Maria L},
abstractNote = {Ferredoxin5 (FDX5), a minor ferredoxin protein in the alga, Chlamydomonas (Chlamydomonas reinhardtii), helps maintain thylakoid membrane integrity in the dark. Sulfur (S) deprivation has been used to achieve prolonged hydrogen production in green algae. Here, we propose that FDX5 is involved in algal responses to sulfur-deprivation as well as to the dark. Specifically, we tested the role of FDX5 in both the initial aerobic and subsequent anaerobic phases of S-deprivation. Under S-deprived conditions, absence of FDX5 causes a distinct delay in achieving anoxia by affecting photosynthetic O2 evolution, accompanied by reduced acetate uptake, lower starch accumulation, and delayed/lower fermentative metabolite production, including photohydrogen. We attribute these differences to transcriptional and/or post-translational regulation of acetyl-CoA synthetase and ADP-glucose pyrophosphorylase, and increased stability of the Photosystem II D1 protein. Interestingly, increased levels of Ferredoxin2 and Ferredoxin1 were observed in the mutant under oxic, S-replete conditions, strengthening our previously proposed hypothesis that other ferredoxins compensate in response to a lack of FDX5. Taken together, the results of our omics and pull-down experiments confirmed biochemical and physiological results, suggesting that FDX5 may have other effects on Chlamydomonas metabolism through its interaction with multiple redox partners.},
doi = {10.1104/pp.19.00457},
journal = {Plant Physiology},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {7}
}