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Title: Gene editing of Gluconobacter oxydans for improved xylose metabolism and bioleaching

Abstract

Rare earth elements can be recycled using bioleaching methods that employ agricultural waste as the carbon source. One of the most abundant sources of this waste is corn stover, which contains both glucose and xylose sugars. The chosen bacteria, Gluconobacter oxydans (G. ox), has low efficiency for metabolizing xylose, which can affect the concentration of organic acids produced. This research focused on the introduction of a plasmid for xylose metabolism into the G. ox genome. A triparental mating method was used. Success of the conjugation was verified with a plasmid prep and restriction digest. The restriction digest suggested some unexpected damage to the plasmid DNA, which will require some backtracking to remedy. In the future, the cells with altered genetics will be used in experimentation with the production of corn stover lixiviant.

Authors:
 [1]; ORCiD logo [1]; ORCiD logo [1]
  1. Idaho National Laboratory
Publication Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1546711
Report Number(s):
INL/EXP-19-55116-Rev000
DOE Contract Number:  
AC07-05ID14517
Resource Type:
Conference
Resource Relation:
Conference: INL Intern Poster Session, EIL A102, 08/08/2019 - 08/08/2019
Country of Publication:
United States
Language:
English
Subject:
54 - ENVIRONMENTAL SCIENCES/GLOBAL CLIMATE CHANGE STUDIES AND CLIMATE MITIGATION; 59 - BASIC BIOLOGICAL SCIENCES/GENOMICS/GENOME RESEARCH; Rare Earth Elements; Bioleach; Gluconobacter oxydans; Genomics; Plasmid; Corn Stover; Critical Materials

Citation Formats

Scalzone, Katherine R, Reed, David W, and Daubaras, Dayna L. Gene editing of Gluconobacter oxydans for improved xylose metabolism and bioleaching. United States: N. p., 2019. Web.
Scalzone, Katherine R, Reed, David W, & Daubaras, Dayna L. Gene editing of Gluconobacter oxydans for improved xylose metabolism and bioleaching. United States.
Scalzone, Katherine R, Reed, David W, and Daubaras, Dayna L. Thu . "Gene editing of Gluconobacter oxydans for improved xylose metabolism and bioleaching". United States. https://www.osti.gov/servlets/purl/1546711.
@article{osti_1546711,
title = {Gene editing of Gluconobacter oxydans for improved xylose metabolism and bioleaching},
author = {Scalzone, Katherine R and Reed, David W and Daubaras, Dayna L},
abstractNote = {Rare earth elements can be recycled using bioleaching methods that employ agricultural waste as the carbon source. One of the most abundant sources of this waste is corn stover, which contains both glucose and xylose sugars. The chosen bacteria, Gluconobacter oxydans (G. ox), has low efficiency for metabolizing xylose, which can affect the concentration of organic acids produced. This research focused on the introduction of a plasmid for xylose metabolism into the G. ox genome. A triparental mating method was used. Success of the conjugation was verified with a plasmid prep and restriction digest. The restriction digest suggested some unexpected damage to the plasmid DNA, which will require some backtracking to remedy. In the future, the cells with altered genetics will be used in experimentation with the production of corn stover lixiviant.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {8}
}

Conference:
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