Structural organization of a Type III-A CRISPR effector subcomplex determined by X-ray crystallography and cryo-EM

Dorsey, Bryan W.; Huang, Lei; Mondragón, Alfonso

doi:10.1093/nar/gkz079

Title: Structural organization of a Type III-A CRISPR effector subcomplex determined by X-ray crystallography and cryo-EM

Journal Article · Wed Feb 13 00:00:00 EST 2019 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkz079· OSTI ID:1544886

Dorsey, Bryan W. ^[1]; Huang, Lei ^[1];

^[1]

Northwestern Univ., Evanston, IL (United States)

Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated Cas proteins provide an immune-like response in many prokaryotes against extraneous nucleic acids. CRISPR–Cas systems are classified into different classes and types. Class 1 CRISPR–Cas systems form multi-protein effector complexes that includes a guide RNA (crRNA) used to identify the target for destruction. Here we present crystal structures of Staphylococcus epidermidis Type III-A CRISPR subunits Csm2 and Csm3 and a 5.2 Å resolution single-particle cryo-electron microscopy (cryo-EM) reconstruction of an in vivo assembled effector subcomplex including the crRNA. The structures help to clarify the quaternary architecture of Type III-A effector complexes, and provide details on crRNA binding, target RNA binding and cleavage, and intermolecular interactions essential for effector complex assembly. The structures allow a better understanding of the organization of Type III-A CRISPR effector complexes as well as highlighting the overall similarities and differences with other Class 1 effector complexes.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH); Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor; R.H. Lurie Comprehensive Cancer Center of Northwestern University; Searle Funds at The Chicago Community Trust; National Center for Research Resources

Grant/Contract Number:: R35-GM118108; S10RR027842

OSTI ID:: 1544886

Journal Information:: Nucleic Acids Research, Vol. 47, Issue 7; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 6 works

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