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Title: Assessing microbial community patterns under incipient soil formation from basalt

Abstract

Microbial dynamics drive the biotic machinery of early soil evolution. However, integrated knowledge of microbial community establishment, functional associations, and community assembly processes in incipient soil is lacking. This study presents a novel approach of combining microbial phylogenetic profiling, functional predictions, and community assembly processes to analyze drivers of microbial community establishment in an emerging soil system. Rigorous sub-meter sampling of a basalt-soil lysimeter after two years of irrigation revealed the contrasting depth-dependent distribution patterns of soil parameters and community diversity. Phylogenetic analysis of 16S rRNA gene indicated the presence of diverse bacterial and archaeal phyla, with high relative abundance of Actinobacteria on the surface and a consistently high abundance of Proteobacteria at all depths. Predicted functional gene analysis suggested dominant autotrophy signatures, evidence of an incomplete nitrogen cycle, and predicted enzymes of iron (III) to iron (II) conversion followed by intracellular uptake, transport, and regulation. Null modeling revealed microbial community assembly predominantly governed by variable selection. The relative influence of the variable selection decreased with depth, indicating unique and relatively harsh environmental conditions near the surface and more benign conditions with depth. Additionally, high levels of dispersal influenced community composition near the center of the domain, suggesting that spatialmore » processes interact with deterministic selection imposed by the environment. Characterizing the complexity in incipient soils can advance research insights about microbial community diversity and assembly in other incipient systems (e.g., transformation of marginal soils, or soils recovering from disturbances such as fire, erosion, intensive agricultural practices, or contamination).« less

Authors:
ORCiD logo [1]; ORCiD logo [2];  [3]; ;  [3];  [4];  [3];  [3];  [3];  [3];  [3]
  1. UNIVERSITY OF ARIZONA
  2. BATTELLE (PACIFIC NW LAB)
  3. University of Arizona
  4. University of Alaska Anchorage
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1543313
Report Number(s):
PNNL-SA-127573
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Journal of Geophysical Research. Biogeosciences
Additional Journal Information:
Journal Volume: 124; Journal Issue: 4
Country of Publication:
United States
Language:
English

Citation Formats

Sengupta, Aditi, Stegen, James C., Neto, Antonio, Wang, Yadi, Neilson, Julia W., Tatarin, Triffon, Hunt, Edward A., Dontsova, Katerina, Chorover, Jon D., Troch, Peter, and Maier, Raina M. Assessing microbial community patterns under incipient soil formation from basalt. United States: N. p., 2019. Web. doi:10.1029/2017JG004315.
Sengupta, Aditi, Stegen, James C., Neto, Antonio, Wang, Yadi, Neilson, Julia W., Tatarin, Triffon, Hunt, Edward A., Dontsova, Katerina, Chorover, Jon D., Troch, Peter, & Maier, Raina M. Assessing microbial community patterns under incipient soil formation from basalt. United States. doi:10.1029/2017JG004315.
Sengupta, Aditi, Stegen, James C., Neto, Antonio, Wang, Yadi, Neilson, Julia W., Tatarin, Triffon, Hunt, Edward A., Dontsova, Katerina, Chorover, Jon D., Troch, Peter, and Maier, Raina M. Fri . "Assessing microbial community patterns under incipient soil formation from basalt". United States. doi:10.1029/2017JG004315.
@article{osti_1543313,
title = {Assessing microbial community patterns under incipient soil formation from basalt},
author = {Sengupta, Aditi and Stegen, James C. and Neto, Antonio and Wang, Yadi and Neilson, Julia W. and Tatarin, Triffon and Hunt, Edward A. and Dontsova, Katerina and Chorover, Jon D. and Troch, Peter and Maier, Raina M.},
abstractNote = {Microbial dynamics drive the biotic machinery of early soil evolution. However, integrated knowledge of microbial community establishment, functional associations, and community assembly processes in incipient soil is lacking. This study presents a novel approach of combining microbial phylogenetic profiling, functional predictions, and community assembly processes to analyze drivers of microbial community establishment in an emerging soil system. Rigorous sub-meter sampling of a basalt-soil lysimeter after two years of irrigation revealed the contrasting depth-dependent distribution patterns of soil parameters and community diversity. Phylogenetic analysis of 16S rRNA gene indicated the presence of diverse bacterial and archaeal phyla, with high relative abundance of Actinobacteria on the surface and a consistently high abundance of Proteobacteria at all depths. Predicted functional gene analysis suggested dominant autotrophy signatures, evidence of an incomplete nitrogen cycle, and predicted enzymes of iron (III) to iron (II) conversion followed by intracellular uptake, transport, and regulation. Null modeling revealed microbial community assembly predominantly governed by variable selection. The relative influence of the variable selection decreased with depth, indicating unique and relatively harsh environmental conditions near the surface and more benign conditions with depth. Additionally, high levels of dispersal influenced community composition near the center of the domain, suggesting that spatial processes interact with deterministic selection imposed by the environment. Characterizing the complexity in incipient soils can advance research insights about microbial community diversity and assembly in other incipient systems (e.g., transformation of marginal soils, or soils recovering from disturbances such as fire, erosion, intensive agricultural practices, or contamination).},
doi = {10.1029/2017JG004315},
journal = {Journal of Geophysical Research. Biogeosciences},
number = 4,
volume = 124,
place = {United States},
year = {2019},
month = {4}
}

Works referenced in this record:

Adaptation, Niche Conservatism, and Convergence: Comparative Studies of Leaf Evolution in the California Chaparral
journal, May 2004

  • Ackerly, David D.
  • The American Naturalist, Vol. 163, Issue 5
  • DOI: 10.1086/383062

Thousands of microbial genomes shed light on interconnected biogeochemical processes in an aquifer system
journal, October 2016

  • Anantharaman, Karthik; Brown, Christopher T.; Hug, Laura A.
  • Nature Communications, Vol. 7, Issue 1
  • DOI: 10.1038/ncomms13219

Expanded diversity of microbial groups that shape the dissimilatory sulfur cycle
journal, February 2018

  • Anantharaman, Karthik; Hausmann, Bela; Jungbluth, Sean P.
  • The ISME Journal, Vol. 12, Issue 7
  • DOI: 10.1038/s41396-018-0078-0

Biological impact on mineral dissolution: Application of the lichen model to understanding mineral weathering in the rhizosphere
journal, March 1999

  • Banfield, J. F.; Barker, W. W.; Welch, S. A.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 7
  • DOI: 10.1073/pnas.96.7.3404

Habitat Selection in Plants
journal, June 1991


Neutral Macroecology
journal, September 2001


Deep-biosphere consortium of fungi and prokaryotes in Eocene subseafloor basalts
journal, September 2014

  • Bengtson, S.; Ivarsson, M.; Astolfo, A.
  • Geobiology, Vol. 12, Issue 6
  • DOI: 10.1111/gbi.12100

Soil-forming factors and Soil Taxonomy
journal, August 2014


Microbial community dynamics in the forefield of glaciers
journal, November 2014

  • Bradley, James A.; Singarayer, Joy S.; Anesio, Alexandre M.
  • Proceedings of the Royal Society B: Biological Sciences, Vol. 281, Issue 1795
  • DOI: 10.1098/rspb.2014.0882

Contrasting primary successional trajectories of fungi and bacteria in retreating glacier soils
journal, September 2013

  • Brown, Shawn P.; Jumpponen, Ari
  • Molecular Ecology, Vol. 23, Issue 2
  • DOI: 10.1111/mec.12487

Dissimilatory Nitrate Reduction to Ammonium in the Yellow River Estuary: Rates, Abundance, and Community Diversity
journal, July 2017


Bacterial community assembly based on functional genes rather than species
journal, August 2011

  • Burke, C.; Steinberg, P.; Rusch, D.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 34
  • DOI: 10.1073/pnas.1101591108

PyNAST: a flexible tool for aligning sequences to a template alignment
journal, November 2009


QIIME allows analysis of high-throughput community sequencing data
journal, April 2010

  • Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
  • Nature Methods, Vol. 7, Issue 5
  • DOI: 10.1038/nmeth.f.303

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012

  • Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A.
  • The ISME Journal, Vol. 6, Issue 8
  • DOI: 10.1038/ismej.2012.8

Stochastic and deterministic processes interact in the assembly of desert microbial communities on a global scale
journal, March 2011

  • Caruso, Tancredi; Chan, Yuki; Lacap, Donnabella C.
  • The ISME Journal, Vol. 5, Issue 9
  • DOI: 10.1038/ismej.2011.21

Biogeochemical drivers of microbial community convergence across actively retreating glaciers
journal, October 2016


Phylogenetic Overdispersion in Floridian Oak Communities
journal, June 2004

  • Cavender‐Bares, J.; Ackerly, D. D.; Baum, D. A.
  • The American Naturalist, Vol. 163, Issue 6
  • DOI: 10.1086/386375

The merging of community ecology and phylogenetic biology
journal, July 2009


Bacteria in Weathered Basaltic Glass, Iceland
journal, September 2009

  • Cockell, Charles S.; Olsson, Karen; Knowles, Felicity
  • Geomicrobiology Journal, Vol. 26, Issue 7
  • DOI: 10.1080/01490450903061101

Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
journal, July 2006

  • DeSantis, T. Z.; Hugenholtz, P.; Larsen, N.
  • Applied and Environmental Microbiology, Vol. 72, Issue 7, p. 5069-5072
  • DOI: 10.1128/AEM.03006-05

Disentangling mechanisms that mediate the balance between stochastic and deterministic processes in microbial succession
journal, March 2015

  • Dini-Andreote, Francisco; Stegen, James C.; van Elsas, Jan Dirk
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 11
  • DOI: 10.1073/pnas.1414261112

A phylogenetic perspective on the distribution of plant diversity
journal, August 2008

  • Donoghue, M. J.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue Supplement 1
  • DOI: 10.1073/pnas.0801962105

Solid phase evolution in the Biosphere 2 hillslope experiment as predicted by modeling of hydrologic and geochemical fluxes
journal, January 2009

  • Dontsova, K.; Steefel, C. I.; Desilets, S.
  • Hydrology and Earth System Sciences, Vol. 13, Issue 12
  • DOI: 10.5194/hess-13-2273-2009

Relative roles of niche and neutral processes in structuring a soil microbial community
journal, November 2009

  • Dumbrell, Alex J.; Nelson, Michaela; Helgason, Thorunn
  • The ISME Journal, Vol. 4, Issue 3
  • DOI: 10.1038/ismej.2009.122

Microbial dispersal in unsaturated porous media: Characteristics of motile bacterial cell motions in unsaturated angular pore networks
journal, September 2014


Search and clustering orders of magnitude faster than BLAST
journal, August 2010


Phylogenetic analysis of community assembly and structure over space and time
journal, November 2008


Modeling Macroscopic Patterns in Ecology
journal, March 2002


Functional equivalence and evolutionary convergence in complex communities of microbial sponge symbionts
journal, June 2012

  • Fan, L.; Reynolds, D.; Liu, M.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 27
  • DOI: 10.1073/pnas.1203287109

Cross-biome metagenomic analyses of soil microbial communities and their functional attributes
journal, December 2012

  • Fierer, N.; Leff, J. W.; Adams, B. J.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 52
  • DOI: 10.1073/pnas.1215210110

The Growth–Defense Trade-Off and Habitat Specialization by Plants in Amazonian Forests
journal, July 2006


Microbial community structure and its functional implications
journal, May 2009


Community Assembly Through Adaptive Radiation in Hawaiian Spiders
journal, January 2004


Site- and horizon-specific patterns of microbial community structure and enzyme activities in permafrost-affected soils of Greenland
journal, October 2014

  • Gittel, Antje; Bárta, Jiří; Kohoutová, Iva
  • Frontiers in Microbiology, Vol. 5
  • DOI: 10.3389/fmicb.2014.00541

Phylogenetic beta diversity: linking ecological and evolutionary processes across space in time
journal, December 2008


Spatial scaling of microbial eukaryote diversity
journal, December 2004

  • Green, Jessica L.; Holmes, Andrew J.; Westoby, Mark
  • Nature, Vol. 432, Issue 7018
  • DOI: 10.1038/nature03034

A soil actinobacterium scavenges atmospheric H2 using two membrane-associated, oxygen-dependent [NiFe] hydrogenases
journal, March 2014

  • Greening, C.; Berney, M.; Hards, K.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 11
  • DOI: 10.1073/pnas.1320586111

Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons
journal, January 2011


Changes in Bacterial and Archaeal Community Structure and Functional Diversity along a Geochemically Variable Soil Profile
journal, January 2008

  • Hansel, C. M.; Fendorf, S.; Jardine, P. M.
  • Applied and Environmental Microbiology, Vol. 74, Issue 5
  • DOI: 10.1128/AEM.01787-07

Beyond biogeographic patterns: processes shaping the microbial landscape
journal, May 2012

  • Hanson, China A.; Fuhrman, Jed A.; Horner-Devine, M. Claire
  • Nature Reviews Microbiology, Vol. 10, Issue 7
  • DOI: 10.1038/nrmicro2795

Bacterial, archaeal and eukaryal community structures throughout soil horizons of harvested and naturally disturbed forest stands
journal, December 2009


Occupancy, Spatial Variance, and the Abundance of Species
journal, September 2003

  • He, Fangliang; Gaston, Kevin J.
  • The American Naturalist, Vol. 162, Issue 3
  • DOI: 10.1086/377190

A taxa–area relationship for bacteria
journal, December 2004

  • Horner-Devine, M. Claire; Lage, Melissa; Hughes, Jennifer B.
  • Nature, Vol. 432, Issue 7018
  • DOI: 10.1038/nature03073

Neutral Theory and the Evolution of Ecological Equivalence
journal, June 2006


Successional Changes in Bacterial Assemblage Structure During Epilithic Biofilm Development
journal, February 2001


Identifying the Dominant Soil Bacterial Taxa in Libraries of 16S rRNA and 16S rRNA Genes
journal, March 2006


Factors of Soil Formation
journal, January 1941


Species co-existence and character divergence across carnivores
journal, February 2007


KEGG: new perspectives on genomes, pathways, diseases and drugs
journal, November 2016

  • Kanehisa, Minoru; Furumichi, Miho; Tanabe, Mao
  • Nucleic Acids Research, Vol. 45, Issue D1
  • DOI: 10.1093/nar/gkw1092

Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences
journal, August 2013

  • Langille, Morgan G. I.; Zaneveld, Jesse; Caporaso, J. Gregory
  • Nature Biotechnology, Vol. 31, Issue 9
  • DOI: 10.1038/nbt.2676

Reduced Epithelial Na+/H+ Exchange Drives Gut Microbial Dysbiosis and Promotes Inflammatory Response in T Cell-Mediated Murine Colitis
journal, April 2016


Does functional redundancy exist?
journal, March 2004


UniFrac: a New Phylogenetic Method for Comparing Microbial Communities
journal, December 2005


Diversity, stability and resilience of the human gut microbiota
journal, September 2012

  • Lozupone, Catherine A.; Stombaugh, Jesse I.; Gordon, Jeffrey I.
  • Nature, Vol. 489, Issue 7415
  • DOI: 10.1038/nature11550

A Nitrospira metagenome illuminates the physiology and evolution of globally important nitrite-oxidizing bacteria
journal, July 2010

  • Lucker, S.; Wagner, M.; Maixner, F.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 30
  • DOI: 10.1073/pnas.1003860107

Mineral–microbe interactions: Biotechnological potential of bioweathering
journal, February 2012


Long-Term Succession of Structure and Diversity of a Biofilm Formed in a Model Drinking Water Distribution System
journal, November 2003


phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data
journal, April 2013


Soil Habitat and Horizon Properties Impact Bacterial Diversity and Composition
journal, January 2011

  • Michel, Himaya M.; Williams, Mark A.
  • Soil Science Society of America Journal, Vol. 75, Issue 4
  • DOI: 10.2136/sssaj2010.0171

Evolution and the latitudinal diversity gradient: speciation, extinction and biogeography
journal, April 2007


Significant Impacts of Increasing Aridity on the Arid Soil Microbiome
journal, May 2017


Life at the hyperarid margin: novel bacterial diversity in arid soils of the Atacama Desert, Chile
journal, April 2012


Global biogeography of microbial nitrogen-cycling traits in soil
journal, July 2016

  • Nelson, Michaeline B.; Martiny, Adam C.; Martiny, Jennifer B. H.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 29
  • DOI: 10.1073/pnas.1601070113

Patterns and Processes of Microbial Community Assembly
journal, September 2013

  • Nemergut, D. R.; Schmidt, S. K.; Fukami, T.
  • Microbiology and Molecular Biology Reviews, Vol. 77, Issue 3
  • DOI: 10.1128/MMBR.00051-12

The effect of rock composition on cyanobacterial weathering of crystalline basalt and rhyolite
journal, June 2012


Microbial Ecology of the Dark Ocean above, at, and below the Seafloor
journal, June 2011

  • Orcutt, B. N.; Sylvan, J. B.; Knab, N. J.
  • Microbiology and Molecular Biology Reviews, Vol. 75, Issue 2
  • DOI: 10.1128/MMBR.00039-10

Making a living while starving in the dark: metagenomic insights into the energy dynamics of a carbonate cave
journal, September 2013

  • Ortiz, Marianyoly; Legatzki, Antje; Neilson, Julia W.
  • The ISME Journal, Vol. 8, Issue 2
  • DOI: 10.1038/ismej.2013.159

The Landscape Evolution Observatory: A large-scale controllable infrastructure to study coupled Earth-surface processes
journal, September 2015


The mechanistic basis for storage-dependent age distributions of water discharged from an experimental hillslope: MECHANISTIC BASIS FOR AGE DISTRIBUTIONS
journal, April 2017

  • Pangle, Luke A.; Kim, Minseok; Cardoso, Charlene
  • Water Resources Research, Vol. 53, Issue 4
  • DOI: 10.1002/2016WR019901

Pore water chemistry reveals gradients in mineral transformation across a model basaltic hillslope: BASALTIC PORE WATER CHEMISTRY
journal, June 2016

  • Pohlmann, Michael; Dontsova, Katerina; Root, Robert
  • Geochemistry, Geophysics, Geosystems, Vol. 17, Issue 6
  • DOI: 10.1002/2016GC006270

Methylotrophic methanogenic Thermoplasmata implicated in reduced methane emissions from bovine rumen
journal, February 2013

  • Poulsen, Morten; Schwab, Clarissa; Borg Jensen, Bent
  • Nature Communications, Vol. 4, Issue 1
  • DOI: 10.1038/ncomms2432

FastTree 2 – Approximately Maximum-Likelihood Trees for Large Alignments
journal, March 2010


A comprehensive framework for global patterns in biodiversity
journal, January 2004


The case for ecological neutral theory
journal, April 2012

  • Rosindell, James; Hubbell, Stephen P.; He, Fangliang
  • Trends in Ecology & Evolution, Vol. 27, Issue 4
  • DOI: 10.1016/j.tree.2012.01.004

Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
journal, October 2009

  • Schloss, P. D.; Westcott, S. L.; Ryabin, T.
  • Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541
  • DOI: 10.1128/AEM.01541-09

Do bacterial and fungal communities assemble differently during primary succession?
journal, January 2014

  • Schmidt, S. K.; Nemergut, D. R.; Darcy, J. L.
  • Molecular Ecology, Vol. 23, Issue 2
  • DOI: 10.1111/mec.12589

Soil Lysimeter Excavation for Coupled Hydrological, Geochemical, and Microbiological Investigations
journal, January 2016

  • Sengupta, Aditi; Wang, Yadi; Meira Neto, Antonio A.
  • Journal of Visualized Experiments, Issue 115
  • DOI: 10.3791/54536

Depth-dependent influence of different land-use systems on bacterial biogeography
journal, December 2016

  • Seuradge, Brent J.; Oelbermann, Maren; Neufeld, Josh D.
  • FEMS Microbiology Ecology, Vol. 93, Issue 2
  • DOI: 10.1093/femsec/fiw239

Quantifying the roles of immigration and chance in shaping prokaryote community structure
journal, April 2006


Modeling Taxa-Abundance Distributions in Microbial Communities using Environmental Sequence Data
journal, December 2006


Microbial community assembly and evolution in subseafloor sediment
journal, February 2017

  • Starnawski, Piotr; Bataillon, Thomas; Ettema, Thijs J. G.
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 11
  • DOI: 10.1073/pnas.1614190114

Groundwater–surface water mixing shifts ecological assembly processes and stimulates organic carbon turnover
journal, April 2016

  • Stegen, James C.; Fredrickson, James K.; Wilkins, Michael J.
  • Nature Communications, Vol. 7, Issue 1
  • DOI: 10.1038/ncomms11237

Coupling among Microbial Communities, Biogeochemistry and Mineralogy across Biogeochemical Facies
journal, July 2016

  • Stegen, James C.; Konopka, Allan; McKinley, James P.
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep30553

Quantifying community assembly processes and identifying features that impose them
journal, June 2013

  • Stegen, James C.; Lin, Xueju; Fredrickson, Jim K.
  • The ISME Journal, Vol. 7, Issue 11
  • DOI: 10.1038/ismej.2013.93

Stochastic and deterministic assembly processes in subsurface microbial communities
journal, March 2012

  • Stegen, James C.; Lin, Xueju; Konopka, Allan E.
  • The ISME Journal, Vol. 6, Issue 9
  • DOI: 10.1038/ismej.2012.22

Exotic taxa less related to native species are more invasive
journal, March 2006

  • Strauss, S. Y.; Webb, C. O.; Salamin, N.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 15
  • DOI: 10.1073/pnas.0508073103

The Influence of Spatial and size Scale on Phylogenetic Relatedness in Tropical Forest Communities
journal, July 2007

  • Swenson, Nathan G.; Enquist, Brian J.; Thompson, Jill
  • Ecology, Vol. 88, Issue 7
  • DOI: 10.1890/06-1499.1

Endemism and functional convergence across the North American soil mycobiome
journal, April 2014

  • Talbot, J. M.; Bruns, T. D.; Taylor, J. W.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 17
  • DOI: 10.1073/pnas.1402584111

Biophysical processes supporting the diversity of microbial life in soil
journal, August 2017


Disguised as a Sulfate Reducer: Growth of the Deltaproteobacterium Desulfurivibrio alkaliphilus by Sulfide Oxidation with Nitrate
journal, July 2017


Niche tradeoffs, neutrality, and community structure: A stochastic theory of resource competition, invasion, and community assembly
journal, July 2004


Emerging patterns in the comparative analysis of phylogenetic community structure
journal, February 2009


Micro-scale determinants of bacterial diversity in soil
journal, November 2013

  • Vos, Michiel; Wolf, Alexandra B.; Jennings, Sarah J.
  • FEMS Microbiology Reviews, Vol. 37, Issue 6
  • DOI: 10.1111/1574-6976.12023

Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy
journal, June 2007

  • Wang, Q.; Garrity, G. M.; Tiedje, J. M.
  • Applied and Environmental Microbiology, Vol. 73, Issue 16
  • DOI: 10.1128/AEM.00062-07

Molecular analyses of microbial diversity associated with the Lonar soda lake in India: An impact crater in a basalt area
journal, December 2006

  • Wani, Aijaz Ahmad; Surakasi, Venkata Prasad; Siddharth, Jay
  • Research in Microbiology, Vol. 157, Issue 10
  • DOI: 10.1016/j.resmic.2006.08.005

Phylogenies and Community Ecology
journal, November 2002


Community composition and seasonal changes of archaea in coarse and fine air particulate matter
journal, January 2018

  • Wehking, Jörn; Pickersgill, Daniel A.; Bowers, Robert M.
  • Biogeosciences, Vol. 15, Issue 13
  • DOI: 10.5194/bg-15-4205-2018

Horizon-Specific Bacterial Community Composition of German Grassland Soils, as Revealed by Pyrosequencing-Based Analysis of 16S rRNA Genes
journal, August 2010

  • Will, C.; Thurmer, A.; Wollherr, A.
  • Applied and Environmental Microbiology, Vol. 76, Issue 20
  • DOI: 10.1128/AEM.01063-10

Neutral assembly of bacterial communities: Neutral assembly of bacterial communities
journal, November 2007