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Title: Alterations in the relative abundance of Faecalibacterium prausnitzii correlate with changes in fecal calprotectin in patients with ileal Crohn’s disease: a longitudinal study [Increasing abundance of Faecalibacterium prausnitzii associated with a reduced degree of intestinal inflammation in Crohn’s disease: A longitudinal study]

Abstract

Objectives: Crohn’s disease is characterized by a gut dysbiosis with decreased abundance of butyrate producers such as Faecalibacterium prausnitzii. Although F. prausnitzii secretes anti-inflammatory molecules, few studies have addressed the importance of F. prausnitzii in a longitudinal setting. We aimed to examine the relationship between temporal profiles of F. prausnitzii, the C. leptum group, overall butyrate production, and inflammatory activity. Material and methods: Fecal samples ( n = 59) were collected every third month from nine patients with ileal Crohn’s disease. The abundance of F. prausnitzii and C. leptum was quantified relative to the total amount of bacteria using quantitative-PCR. To assess butyrate production of gut microbiota, gene copy numbers of the butyryl-CoA:acetate-CoA transferase (BCoAT) gene were quantified by qPCR. The inflammatory activity was defined by fecal (f)-calprotectin. Results: No correlation between the relative abundance of F. prausnitzii, the C. leptum group, or copy numbers of the BCoAT gene, and f-calprotectin was observed in the total sample set. By analyzing alterations between consecutive samples, a negative correlation between changes in the relative abundance of F. prausnitzii and f-calprotectin was observed ( R = 0.39; p = .009). Changes in C. leptum ( R = 0.18, p = .23) and numbermore » of copies of the BCoAT gene ( R = 0.12; p = .42) did not correlate with f-calprotectin. Conclusions: There was an inverse correlation between temporal changes in the relative abundance of F. prausnitzii, but not overall butyrate producing capacity, and changes in inflammatory activity in ileal Crohn’s disease. These findings indicate that F. prausnitzii may play a role in gut homeostasis, even though causality is still to be demonstrated.« less

Authors:
ORCiD logo [1];  [1];  [2]; ORCiD logo [3];  [3];  [2];  [1];  [1]
  1. Örebro Univ., Örebro (Sweden)
  2. Karolinska Institutet, Stockholm (Sweden)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1543275
Report Number(s):
PNNL-SA-139272
Journal ID: ISSN 0036-5521
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scandinavian Journal of Gastroenterology
Additional Journal Information:
Journal Volume: 54; Journal Issue: 5; Journal ID: ISSN 0036-5521
Publisher:
Taylor & Francis
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; Faecalibacterium prausnitzii; crohn's disease; Calprotectin; butyrate; dysbiosis

Citation Formats

Björkqvist, Olle, Repsilber, Dirk, Seifert, Maike, Brislawn, Colin J., Jansson, Janet K., Engstrand, Lars, Rangel, Ignacio, and Halfvarson, Jonas. Alterations in the relative abundance of Faecalibacterium prausnitzii correlate with changes in fecal calprotectin in patients with ileal Crohn’s disease: a longitudinal study [Increasing abundance of Faecalibacterium prausnitzii associated with a reduced degree of intestinal inflammation in Crohn’s disease: A longitudinal study]. United States: N. p., 2019. Web. doi:10.1080/00365521.2019.1599417.
Björkqvist, Olle, Repsilber, Dirk, Seifert, Maike, Brislawn, Colin J., Jansson, Janet K., Engstrand, Lars, Rangel, Ignacio, & Halfvarson, Jonas. Alterations in the relative abundance of Faecalibacterium prausnitzii correlate with changes in fecal calprotectin in patients with ileal Crohn’s disease: a longitudinal study [Increasing abundance of Faecalibacterium prausnitzii associated with a reduced degree of intestinal inflammation in Crohn’s disease: A longitudinal study]. United States. https://doi.org/10.1080/00365521.2019.1599417
Björkqvist, Olle, Repsilber, Dirk, Seifert, Maike, Brislawn, Colin J., Jansson, Janet K., Engstrand, Lars, Rangel, Ignacio, and Halfvarson, Jonas. Wed . "Alterations in the relative abundance of Faecalibacterium prausnitzii correlate with changes in fecal calprotectin in patients with ileal Crohn’s disease: a longitudinal study [Increasing abundance of Faecalibacterium prausnitzii associated with a reduced degree of intestinal inflammation in Crohn’s disease: A longitudinal study]". United States. https://doi.org/10.1080/00365521.2019.1599417. https://www.osti.gov/servlets/purl/1543275.
@article{osti_1543275,
title = {Alterations in the relative abundance of Faecalibacterium prausnitzii correlate with changes in fecal calprotectin in patients with ileal Crohn’s disease: a longitudinal study [Increasing abundance of Faecalibacterium prausnitzii associated with a reduced degree of intestinal inflammation in Crohn’s disease: A longitudinal study]},
author = {Björkqvist, Olle and Repsilber, Dirk and Seifert, Maike and Brislawn, Colin J. and Jansson, Janet K. and Engstrand, Lars and Rangel, Ignacio and Halfvarson, Jonas},
abstractNote = {Objectives: Crohn’s disease is characterized by a gut dysbiosis with decreased abundance of butyrate producers such as Faecalibacterium prausnitzii. Although F. prausnitzii secretes anti-inflammatory molecules, few studies have addressed the importance of F. prausnitzii in a longitudinal setting. We aimed to examine the relationship between temporal profiles of F. prausnitzii, the C. leptum group, overall butyrate production, and inflammatory activity. Material and methods: Fecal samples (n = 59) were collected every third month from nine patients with ileal Crohn’s disease. The abundance of F. prausnitzii and C. leptum was quantified relative to the total amount of bacteria using quantitative-PCR. To assess butyrate production of gut microbiota, gene copy numbers of the butyryl-CoA:acetate-CoA transferase (BCoAT) gene were quantified by qPCR. The inflammatory activity was defined by fecal (f)-calprotectin. Results: No correlation between the relative abundance of F. prausnitzii, the C. leptum group, or copy numbers of the BCoAT gene, and f-calprotectin was observed in the total sample set. By analyzing alterations between consecutive samples, a negative correlation between changes in the relative abundance of F. prausnitzii and f-calprotectin was observed (R = 0.39; p = .009). Changes in C. leptum (R = 0.18, p = .23) and number of copies of the BCoAT gene (R = 0.12; p = .42) did not correlate with f-calprotectin. Conclusions: There was an inverse correlation between temporal changes in the relative abundance of F. prausnitzii, but not overall butyrate producing capacity, and changes in inflammatory activity in ileal Crohn’s disease. These findings indicate that F. prausnitzii may play a role in gut homeostasis, even though causality is still to be demonstrated.},
doi = {10.1080/00365521.2019.1599417},
url = {https://www.osti.gov/biblio/1543275}, journal = {Scandinavian Journal of Gastroenterology},
issn = {0036-5521},
number = 5,
volume = 54,
place = {United States},
year = {2019},
month = {3}
}

Journal Article:
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Figures / Tables:

Table 1 Table 1: Thermal profiles for quantitative PCR

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Works referenced in this record:

Determinants of Reduced Genetic Capacity for Butyrate Synthesis by the Gut Microbiome in Crohn’s Disease and Ulcerative Colitis
journal, October 2017


Universality of human microbial dynamics
journal, June 2016


Dynamics of metatranscription in the inflammatory bowel disease gut microbiome
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Enteric Salmonellosis Disrupts the Microbial Ecology of the Murine Gastrointestinal Tract
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A Pyrosequencing Study in Twins Shows That Gastrointestinal Microbial Profiles Vary With Inflammatory Bowel Disease Phenotypes
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Low counts of Faecalibacterium prausnitzii in colitis microbiota
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Twin studies reveal specific imbalances in the mucosa-associated microbiota of patients with ileal Crohnʼs disease
journal, January 2009


The Treatment-Naive Microbiome in New-Onset Crohn’s Disease
journal, March 2014


The use of next generation sequencing in the diagnosis and typing of respiratory infections
journal, August 2015


Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
journal, December 2001


Classification of Inflammatory Bowel Disease
journal, January 1989


Mucosal metabolism in ulcerative colitis and crohnʼs disease
journal, January 1998


Potential beneficial effects of butyrate in intestinal and extraintestinal diseases
journal, January 2011


The gut microbiota of siblings offers insights into microbial pathogenesis of inflammatory bowel disease
journal, January 2017


Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients
journal, October 2008


Reduced Abundance of Butyrate-Producing Bacteria Species in the Fecal Microbial Community in Crohn's Disease
journal, January 2016


Correlation Between Concentrations of Fecal Calprotectin and Outcomes of Patients With Ulcerative Colitis in a Phase 2 Trial
journal, January 2016


Active Crohnʼs disease and ulcerative colitis can be specifically diagnosed and monitored based on the biostructure of the fecal flora
journal, January 2008


Role of the faecal stream in the maintenance of Crohn's colitis.
journal, March 1985


The prognostic significance of faecal calprotectin in patients with inactive inflammatory bowel disease
journal, July 2016


Characteristics of Faecal Microbiota in Paediatric Crohn’s Disease and Their Dynamic Changes During Infliximab Therapy
journal, November 2017


Degree of colitis correlates with microbial composition and cytokine responses in colon and caecum of Gαi2-deficient mice
journal, May 2016


Impaired butyrate oxidation in ulcerative colitis is due to decreased butyrate uptake and a defect in the oxidation pathway*
journal, January 2012


Fecal Acetate Is Inversely Related to Acetate Absorption from the Human Rectum and Distal Colon
journal, October 2003


Dynamics of the human gut microbiome in inflammatory bowel disease
journal, February 2017


    Works referencing / citing this record:

    Role of the faecal stream in the maintenance of Crohn's colitis.
    journal, March 1985


    Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
    journal, December 2001


    Impaired butyrate oxidation in ulcerative colitis is due to decreased butyrate uptake and a defect in the oxidation pathway*
    journal, January 2012


    Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients
    journal, October 2008


    Dynamics of the human gut microbiome in inflammatory bowel disease
    journal, February 2017


    The use of next generation sequencing in the diagnosis and typing of respiratory infections
    journal, August 2015


    Fecal Acetate Is Inversely Related to Acetate Absorption from the Human Rectum and Distal Colon
    journal, October 2003


    Universality of human microbial dynamics
    journal, June 2016


    Correlation Between Concentrations of Fecal Calprotectin and Outcomes of Patients With Ulcerative Colitis in a Phase 2 Trial
    journal, January 2016


    Determinants of Reduced Genetic Capacity for Butyrate Synthesis by the Gut Microbiome in Crohn’s Disease and Ulcerative Colitis
    journal, October 2017


    Active Crohnʼs disease and ulcerative colitis can be specifically diagnosed and monitored based on the biostructure of the fecal flora
    journal, January 2008


    Degree of colitis correlates with microbial composition and cytokine responses in colon and caecum of Gαi2-deficient mice
    journal, May 2016


    Dynamics of metatranscription in the inflammatory bowel disease gut microbiome
    journal, January 2018


    A Pyrosequencing Study in Twins Shows That Gastrointestinal Microbial Profiles Vary With Inflammatory Bowel Disease Phenotypes
    journal, December 2010


    Low counts of Faecalibacterium prausnitzii in colitis microbiota
    journal, January 2009


    Twin studies reveal specific imbalances in the mucosa-associated microbiota of patients with ileal Crohnʼs disease
    journal, January 2009


    Reduced Abundance of Butyrate-Producing Bacteria Species in the Fecal Microbial Community in Crohn's Disease
    journal, January 2016


    Classification of Inflammatory Bowel Disease
    journal, January 1989


    The gut microbiota of siblings offers insights into microbial pathogenesis of inflammatory bowel disease
    journal, January 2017


    Potential beneficial effects of butyrate in intestinal and extraintestinal diseases
    journal, January 2011


    Characteristics of Faecal Microbiota in Paediatric Crohn’s Disease and Their Dynamic Changes During Infliximab Therapy
    journal, November 2017


    The prognostic significance of faecal calprotectin in patients with inactive inflammatory bowel disease
    journal, July 2016


    The Treatment-Naive Microbiome in New-Onset Crohn’s Disease
    journal, March 2014