skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Direct Photosynthetic Production of Biodiesel by Growth-Decoupled Cyanobacteria

Abstract

The ultimate goal of this project was the production and excretion of a lauroyl ester-based drop-in biofuel by building on the development of a cyanobacterial strain derived from Synechocystis sp. PCC 6803 that is efficient in using sunlight to reduce CO 2 from the atmosphere to produce and excrete laurate, a fatty acid (FA) that is easily used as a biofuel precursor. To achieve this goal the project focused on five primary objectives: (1) produce more laurate, (2) convert laurate to a lauroyl ester by introducing additional genetic modifications for the synthesis of fatty acid esters, (3) arrest cell growth but not metabolic activity, to make resources more available for lauroyl ester production, (4) reduce the native production of exopolysaccharides to increase the amount of fixed carbon available for biofuel production and to reduce the carbon nutrients available in the medium for potential invasive microbes, and (5) perform a techno-economic analysis to evaluate the longer-term promise and viability of lauroyl ester production in cyanobacterial systems. A photosynthetically produced lauroyl ester could be used as a diesel replacement without the need for additional and costly downstream processing steps, a development of great importance to the mission of DOE with regard tomore » biofuel production.« less

Authors:
 [1]
  1. Arizona State Univ., Mesa, AZ (United States)
Publication Date:
Research Org.:
Arizona State Univ., Mesa, AZ (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1543146
Report Number(s):
EE0007561
DOE Contract Number:  
EE0007561
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; bioctalyst; biofuel; Synechocystis; methyl laurate

Citation Formats

Vermaas, Willem F. J. Direct Photosynthetic Production of Biodiesel by Growth-Decoupled Cyanobacteria. United States: N. p., 2019. Web. doi:10.2172/1543146.
Vermaas, Willem F. J. Direct Photosynthetic Production of Biodiesel by Growth-Decoupled Cyanobacteria. United States. https://doi.org/10.2172/1543146
Vermaas, Willem F. J. Sun . "Direct Photosynthetic Production of Biodiesel by Growth-Decoupled Cyanobacteria". United States. https://doi.org/10.2172/1543146. https://www.osti.gov/servlets/purl/1543146.
@article{osti_1543146,
title = {Direct Photosynthetic Production of Biodiesel by Growth-Decoupled Cyanobacteria},
author = {Vermaas, Willem F. J.},
abstractNote = {The ultimate goal of this project was the production and excretion of a lauroyl ester-based drop-in biofuel by building on the development of a cyanobacterial strain derived from Synechocystis sp. PCC 6803 that is efficient in using sunlight to reduce CO2 from the atmosphere to produce and excrete laurate, a fatty acid (FA) that is easily used as a biofuel precursor. To achieve this goal the project focused on five primary objectives: (1) produce more laurate, (2) convert laurate to a lauroyl ester by introducing additional genetic modifications for the synthesis of fatty acid esters, (3) arrest cell growth but not metabolic activity, to make resources more available for lauroyl ester production, (4) reduce the native production of exopolysaccharides to increase the amount of fixed carbon available for biofuel production and to reduce the carbon nutrients available in the medium for potential invasive microbes, and (5) perform a techno-economic analysis to evaluate the longer-term promise and viability of lauroyl ester production in cyanobacterial systems. A photosynthetically produced lauroyl ester could be used as a diesel replacement without the need for additional and costly downstream processing steps, a development of great importance to the mission of DOE with regard to biofuel production.},
doi = {10.2172/1543146},
url = {https://www.osti.gov/biblio/1543146}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {3}
}