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Title: Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading

Abstract

During phloem unloading, multiple cell-to-cell transport events move organic substances to the root meristem. Although the primary unloading event from the sieve elements to the phloem pole pericycle has been characterized to some extent, little is known about post-sieve element unloading. Here, we report a novel gene, PHLOEM UNLOADING MODULATOR (PLM), in the absence of which plasmodesmata-mediated symplastic transport through the phloem pole pericycle-endodermis interface is specifically enhanced. Increased unloading is attributable to a defect in the formation of the endoplasmic reticulum-plasma membrane tethers during plasmodesmal morphogenesis, resulting in the majority of pores lacking a visible cytoplasmic sleeve. PLM encodes a putative enzyme required for the biosynthesis of sphingolipids with very-long-chain fatty acid. Taken together, our results indicate that post-sieve element unloading involves sphingolipid metabolism, which affects plasmodesmal ultrastructure. They also raise the question of how and why plasmodesmata with no cytoplasmic sleeve facilitate molecular trafficking.

Authors:
ORCiD logo; ; ORCiD logo; ; ; ; ORCiD logo; ; ; ; ; ; ; ORCiD logo; ; ; ; ORCiD logo; ORCiD logo
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1542415
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Nature Plants
Additional Journal Information:
Journal Volume: 5; Journal Issue: 6; Journal ID: ISSN 2055-0278
Country of Publication:
United States
Language:
English

Citation Formats

Yan, Dawei, Yadav, Shri Ram, Paterlini, Andrea, Nicolas, William J., Petit, Jules D., Brocard, Lysiane, Belevich, Ilya, Grison, Magali S., Vaten, Anne, Karami, Leila, el-Showk, Sedeer, Lee, Jung-Youn, Murawska, Gosia M., Mortimer, Jenny, Knoblauch, Michael, Jokitalo, Eija, Markham, Jonathan E., Bayer, Emmanuelle M., and Helariutta, Ykä. Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading. United States: N. p., 2019. Web. doi:10.1038/s41477-019-0429-5.
Yan, Dawei, Yadav, Shri Ram, Paterlini, Andrea, Nicolas, William J., Petit, Jules D., Brocard, Lysiane, Belevich, Ilya, Grison, Magali S., Vaten, Anne, Karami, Leila, el-Showk, Sedeer, Lee, Jung-Youn, Murawska, Gosia M., Mortimer, Jenny, Knoblauch, Michael, Jokitalo, Eija, Markham, Jonathan E., Bayer, Emmanuelle M., & Helariutta, Ykä. Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading. United States. doi:10.1038/s41477-019-0429-5.
Yan, Dawei, Yadav, Shri Ram, Paterlini, Andrea, Nicolas, William J., Petit, Jules D., Brocard, Lysiane, Belevich, Ilya, Grison, Magali S., Vaten, Anne, Karami, Leila, el-Showk, Sedeer, Lee, Jung-Youn, Murawska, Gosia M., Mortimer, Jenny, Knoblauch, Michael, Jokitalo, Eija, Markham, Jonathan E., Bayer, Emmanuelle M., and Helariutta, Ykä. Sat . "Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading". United States. doi:10.1038/s41477-019-0429-5.
@article{osti_1542415,
title = {Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading},
author = {Yan, Dawei and Yadav, Shri Ram and Paterlini, Andrea and Nicolas, William J. and Petit, Jules D. and Brocard, Lysiane and Belevich, Ilya and Grison, Magali S. and Vaten, Anne and Karami, Leila and el-Showk, Sedeer and Lee, Jung-Youn and Murawska, Gosia M. and Mortimer, Jenny and Knoblauch, Michael and Jokitalo, Eija and Markham, Jonathan E. and Bayer, Emmanuelle M. and Helariutta, Ykä},
abstractNote = {During phloem unloading, multiple cell-to-cell transport events move organic substances to the root meristem. Although the primary unloading event from the sieve elements to the phloem pole pericycle has been characterized to some extent, little is known about post-sieve element unloading. Here, we report a novel gene, PHLOEM UNLOADING MODULATOR (PLM), in the absence of which plasmodesmata-mediated symplastic transport through the phloem pole pericycle-endodermis interface is specifically enhanced. Increased unloading is attributable to a defect in the formation of the endoplasmic reticulum-plasma membrane tethers during plasmodesmal morphogenesis, resulting in the majority of pores lacking a visible cytoplasmic sleeve. PLM encodes a putative enzyme required for the biosynthesis of sphingolipids with very-long-chain fatty acid. Taken together, our results indicate that post-sieve element unloading involves sphingolipid metabolism, which affects plasmodesmal ultrastructure. They also raise the question of how and why plasmodesmata with no cytoplasmic sleeve facilitate molecular trafficking.},
doi = {10.1038/s41477-019-0429-5},
journal = {Nature Plants},
issn = {2055-0278},
number = 6,
volume = 5,
place = {United States},
year = {2019},
month = {6}
}

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