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Title: Rhizobacteria mediate the phytotoxicity of a range of biorefineryrelevant compounds

Abstract

Advances in engineering biology have expanded the list of renewable compounds that can be produced at scale via biological routes from plant biomass. In most cases, these chemical products have not been evaluated for effects on biological systems, defined here as bioactivity, that may be relevant to their manufacture. For sustainable chemical and fuel production, the industry needs to transition from fossil to renewable carbon sources, resulting in unprecedented expansion in the production and environmental distribution of chemicals used in biomanufacturing. Further, while some chemicals have been assessed for mammalian toxicity, environmental and agricultural hazards are largely unknown. We assessed six compounds that are representative of the emerging biofuel and bioproduct manufacturing process for their effect on model plants (Arabidopsis thaliana, Sorghum bicolor) and show that several alter plant seedling physiology at sub-millimolar concentrations. However, these responses change in the presence of individual bacterial species from the A. thaliana root microbiome. We identified two individual microbes that change the effect of chemical treatment on root architecture, and a pooled microbial community with different effects relative to its constituents individually. Our findings indicate that screening industrial chemicals for bioactivity on model organisms in the presence of their microbiomes is important formore » biologically and ecologically relevant risk analyses. This article is protected by copyright. All rights reserved.« less

Authors:
 [1];  [1];  [2];  [3];  [3];  [3];  [1];  [3];  [4];  [3];  [3];  [1];  [1]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); San Francisco State Univ., CA (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  4. San Francisco State Univ., CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1542408
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Environmental Toxicology and Chemistry
Additional Journal Information:
Journal Volume: 38; Journal ID: ISSN 0730-7268
Publisher:
SETAC
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Toxicology screening; Microbiome; Plants; Biofuels; Ionic liquids

Citation Formats

Herbert, Robin A., Eng, Thomas, Martinez, Uriel, Wang, Brenda, Langley, Sasha, Wan, Kenneth, Pidatala, Venkataramana, Hoffman, Elijah, Chen, Joseph C., Bissell, Mina J., Brown, James B., Mukhopadhyay, Aindrila, and Mortimer, Jenny C. Rhizobacteria mediate the phytotoxicity of a range of biorefineryrelevant compounds. United States: N. p., 2019. Web. doi:10.1002/etc.4501.
Herbert, Robin A., Eng, Thomas, Martinez, Uriel, Wang, Brenda, Langley, Sasha, Wan, Kenneth, Pidatala, Venkataramana, Hoffman, Elijah, Chen, Joseph C., Bissell, Mina J., Brown, James B., Mukhopadhyay, Aindrila, & Mortimer, Jenny C. Rhizobacteria mediate the phytotoxicity of a range of biorefineryrelevant compounds. United States. doi:10.1002/etc.4501.
Herbert, Robin A., Eng, Thomas, Martinez, Uriel, Wang, Brenda, Langley, Sasha, Wan, Kenneth, Pidatala, Venkataramana, Hoffman, Elijah, Chen, Joseph C., Bissell, Mina J., Brown, James B., Mukhopadhyay, Aindrila, and Mortimer, Jenny C. Fri . "Rhizobacteria mediate the phytotoxicity of a range of biorefineryrelevant compounds". United States. doi:10.1002/etc.4501. https://www.osti.gov/servlets/purl/1542408.
@article{osti_1542408,
title = {Rhizobacteria mediate the phytotoxicity of a range of biorefineryrelevant compounds},
author = {Herbert, Robin A. and Eng, Thomas and Martinez, Uriel and Wang, Brenda and Langley, Sasha and Wan, Kenneth and Pidatala, Venkataramana and Hoffman, Elijah and Chen, Joseph C. and Bissell, Mina J. and Brown, James B. and Mukhopadhyay, Aindrila and Mortimer, Jenny C.},
abstractNote = {Advances in engineering biology have expanded the list of renewable compounds that can be produced at scale via biological routes from plant biomass. In most cases, these chemical products have not been evaluated for effects on biological systems, defined here as bioactivity, that may be relevant to their manufacture. For sustainable chemical and fuel production, the industry needs to transition from fossil to renewable carbon sources, resulting in unprecedented expansion in the production and environmental distribution of chemicals used in biomanufacturing. Further, while some chemicals have been assessed for mammalian toxicity, environmental and agricultural hazards are largely unknown. We assessed six compounds that are representative of the emerging biofuel and bioproduct manufacturing process for their effect on model plants (Arabidopsis thaliana, Sorghum bicolor) and show that several alter plant seedling physiology at sub-millimolar concentrations. However, these responses change in the presence of individual bacterial species from the A. thaliana root microbiome. We identified two individual microbes that change the effect of chemical treatment on root architecture, and a pooled microbial community with different effects relative to its constituents individually. Our findings indicate that screening industrial chemicals for bioactivity on model organisms in the presence of their microbiomes is important for biologically and ecologically relevant risk analyses. This article is protected by copyright. All rights reserved.},
doi = {10.1002/etc.4501},
journal = {Environmental Toxicology and Chemistry},
issn = {0730-7268},
number = ,
volume = 38,
place = {United States},
year = {2019},
month = {7}
}

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