skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Effects of Dissolved Organic Carbon on Copper Toxicity to Embryos of Mytilus galloprovincialis as Measured by Diffusive Gradient in Thin Films

Abstract

Diffusive Gradient in Thin-film (DGT), a passive sampling method, has the potential to better quantify the bioavailable fraction of Cu in seawater, when determining toxicological endpoints, versus grab samples evaluated for total or dissolved Cu. In the present study, laboratory exposure of DGTs and Mediterranean mussel (M. galloprovincialis) embryos at varying concentrations of dissolved organic carbon (DOC) and Cu were performed to resolve the degree to which mimicry of toxicity-buffering occurs in passive sampler quantification. Studies were performed in water collected from Sequim Bay, Washington, USA, spiked with Suwannee River Natural Organic Matter (SRNOM) and Cu(II)SO4. The results provide preliminary EC50s ranging from 4.8 to 11.5 µg/L as CDGT Cu over the span of 0.892 to 8.28 mg/L DOC. M. galloprovincialis was selected as the relevant organism for this correlation based on current consensus for deriving water criteria values, which are based solely from Mytilus spp. toxicological study final acute values, maintaining the intent of protecting environmental and commercial interests to the fauna regarded as most sensitive to Cu input in the marine environment. The present study provides an initial DGT response by which coastal DOC pools with varying functional binding group mixtures can be compared to discern the applicabilitymore » of broad use for quantifying biological effects at time scales integrated to account for pulsed-input to marine waters.« less

Authors:
ORCiD logo [1];  [2];  [3];  [4]
  1. BATTELLE (PACIFIC NW LAB)
  2. SPAWAR
  3. US NAVY
  4. Space and Naval Warfare Systems Center Pacific
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1542087
Report Number(s):
PNNL-SA-139169
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Environmental Toxicology and Chemistry
Additional Journal Information:
Journal Volume: 38; Journal Issue: 5
Country of Publication:
United States
Language:
English
Subject:
copper, Bioavailability, Diffusive Gradient in Thin Films, dissolved organic carbon

Citation Formats

Strivens, Jonathan E., Hayman, Nicholas, Johnston, Robert K., and Rosen, G H. Effects of Dissolved Organic Carbon on Copper Toxicity to Embryos of Mytilus galloprovincialis as Measured by Diffusive Gradient in Thin Films. United States: N. p., 2019. Web. doi:10.1002/etc.4404.
Strivens, Jonathan E., Hayman, Nicholas, Johnston, Robert K., & Rosen, G H. Effects of Dissolved Organic Carbon on Copper Toxicity to Embryos of Mytilus galloprovincialis as Measured by Diffusive Gradient in Thin Films. United States. doi:10.1002/etc.4404.
Strivens, Jonathan E., Hayman, Nicholas, Johnston, Robert K., and Rosen, G H. Wed . "Effects of Dissolved Organic Carbon on Copper Toxicity to Embryos of Mytilus galloprovincialis as Measured by Diffusive Gradient in Thin Films". United States. doi:10.1002/etc.4404.
@article{osti_1542087,
title = {Effects of Dissolved Organic Carbon on Copper Toxicity to Embryos of Mytilus galloprovincialis as Measured by Diffusive Gradient in Thin Films},
author = {Strivens, Jonathan E. and Hayman, Nicholas and Johnston, Robert K. and Rosen, G H.},
abstractNote = {Diffusive Gradient in Thin-film (DGT), a passive sampling method, has the potential to better quantify the bioavailable fraction of Cu in seawater, when determining toxicological endpoints, versus grab samples evaluated for total or dissolved Cu. In the present study, laboratory exposure of DGTs and Mediterranean mussel (M. galloprovincialis) embryos at varying concentrations of dissolved organic carbon (DOC) and Cu were performed to resolve the degree to which mimicry of toxicity-buffering occurs in passive sampler quantification. Studies were performed in water collected from Sequim Bay, Washington, USA, spiked with Suwannee River Natural Organic Matter (SRNOM) and Cu(II)SO4. The results provide preliminary EC50s ranging from 4.8 to 11.5 µg/L as CDGT Cu over the span of 0.892 to 8.28 mg/L DOC. M. galloprovincialis was selected as the relevant organism for this correlation based on current consensus for deriving water criteria values, which are based solely from Mytilus spp. toxicological study final acute values, maintaining the intent of protecting environmental and commercial interests to the fauna regarded as most sensitive to Cu input in the marine environment. The present study provides an initial DGT response by which coastal DOC pools with varying functional binding group mixtures can be compared to discern the applicability of broad use for quantifying biological effects at time scales integrated to account for pulsed-input to marine waters.},
doi = {10.1002/etc.4404},
journal = {Environmental Toxicology and Chemistry},
number = 5,
volume = 38,
place = {United States},
year = {2019},
month = {5}
}