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Title: Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom

Abstract

Oceanic harmful algal blooms ofPseudo-nitzschiadiatoms produce the potent mammalian neurotoxin domoic acid (DA). Despite decades of research, the molecular basis for its biosynthesis is not known. By using growth conditions known to induce DA production inPseudo-nitzschia multiseries, we implemented transcriptome sequencing in order to identify DA biosynthesis genes that colocalize in a genomic four-gene cluster. We biochemically investigated the recombinant DA biosynthetic enzymes and linked their mechanisms to the construction of DA’s diagnostic pyrrolidine skeleton, establishing a model for DA biosynthesis. Knowledge of the genetic basis for toxin production provides an orthogonal approach to bloom monitoring and enables study of environmental factors that drive oceanic DA production.

Authors:
ORCiD logo; ORCiD logo; ORCiD logo; ; ; ORCiD logo; ORCiD logo; ; ; ORCiD logo; ORCiD logo; ORCiD logo; ORCiD logo
Publication Date:
Research Org.:
J. Craig Venter Inst., Inc., Rockville, MD (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1541702
DOE Contract Number:  
SC0008593; SC0018344
Resource Type:
Journal Article
Journal Name:
Science
Additional Journal Information:
Journal Volume: 361; Journal Issue: 6409; Journal ID: ISSN 0036-8075
Publisher:
AAAS
Country of Publication:
United States
Language:
English
Subject:
Science & Technology - Other Topics

Citation Formats

Brunson, John K., McKinnie, Shaun M. K., Chekan, Jonathan R., McCrow, John P., Miles, Zachary D., Bertrand, Erin M., Bielinski, Vincent A., Luhavaya, Hanna, Oborník, Miroslav, Smith, G. Jason, Hutchins, David A., Allen, Andrew E., and Moore, Bradley S. Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom. United States: N. p., 2018. Web. doi:10.1126/science.aau0382.
Brunson, John K., McKinnie, Shaun M. K., Chekan, Jonathan R., McCrow, John P., Miles, Zachary D., Bertrand, Erin M., Bielinski, Vincent A., Luhavaya, Hanna, Oborník, Miroslav, Smith, G. Jason, Hutchins, David A., Allen, Andrew E., & Moore, Bradley S. Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom. United States. https://doi.org/10.1126/science.aau0382
Brunson, John K., McKinnie, Shaun M. K., Chekan, Jonathan R., McCrow, John P., Miles, Zachary D., Bertrand, Erin M., Bielinski, Vincent A., Luhavaya, Hanna, Oborník, Miroslav, Smith, G. Jason, Hutchins, David A., Allen, Andrew E., and Moore, Bradley S. Thu . "Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom". United States. https://doi.org/10.1126/science.aau0382.
@article{osti_1541702,
title = {Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom},
author = {Brunson, John K. and McKinnie, Shaun M. K. and Chekan, Jonathan R. and McCrow, John P. and Miles, Zachary D. and Bertrand, Erin M. and Bielinski, Vincent A. and Luhavaya, Hanna and Oborník, Miroslav and Smith, G. Jason and Hutchins, David A. and Allen, Andrew E. and Moore, Bradley S.},
abstractNote = {Oceanic harmful algal blooms ofPseudo-nitzschiadiatoms produce the potent mammalian neurotoxin domoic acid (DA). Despite decades of research, the molecular basis for its biosynthesis is not known. By using growth conditions known to induce DA production inPseudo-nitzschia multiseries, we implemented transcriptome sequencing in order to identify DA biosynthesis genes that colocalize in a genomic four-gene cluster. We biochemically investigated the recombinant DA biosynthetic enzymes and linked their mechanisms to the construction of DA’s diagnostic pyrrolidine skeleton, establishing a model for DA biosynthesis. Knowledge of the genetic basis for toxin production provides an orthogonal approach to bloom monitoring and enables study of environmental factors that drive oceanic DA production.},
doi = {10.1126/science.aau0382},
url = {https://www.osti.gov/biblio/1541702}, journal = {Science},
issn = {0036-8075},
number = 6409,
volume = 361,
place = {United States},
year = {2018},
month = {9}
}

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