skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Challenges of site-specific selenocysteine incorporation into proteins by Escherichia coli

Journal Article · · RNA Biology
 [1]; ORCiD logo [2]; ORCiD logo [1]
  1. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA
  2. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA; Department of Chemistry, Yale University, New Haven, CT, USA

Not provided.

Research Organization:
Yale Univ., New Haven, CT (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
DOE Contract Number:
FG02-98ER20311
OSTI ID:
1540366
Journal Information:
RNA Biology, Vol. 15, Issue 4-5; ISSN 1547-6286
Publisher:
Taylor & Francis
Country of Publication:
United States
Language:
English

References (90)

Why Nature Chose Selenium journal February 2016
Chemical characterization of the selenoprotein component of clostridial glycine reductase: identification of selenocysteine as the organoselenium moiety. journal August 1976
Early embryonic lethality caused by targeted disruption of the mouse selenocysteine tRNA gene (Trsp) journal May 1997
Selenoproteinless animals: Selenophosphate synthetase SPS1 functions in a pathway unrelated to selenocysteine biosynthesis journal November 2007
The Plasmodium selenoproteome journal January 2006
Adjustments, extinction, and remains of selenocysteine incorporation machinery in the nematode lineage journal May 2014
Relaxation of Selective Constraints Causes Independent Selenoprotein Extinction in Insect Genomes journal August 2008
Comparative genomics reveals new evolutionary and ecological patterns of selenium utilization in bacteria journal January 2016
High content of proteins containing 21st and 22nd amino acids, selenocysteine and pyrrolysine, in a symbiotic deltaproteobacterium of gutless worm Olavius algarvensis journal July 2007
Eukaryotic selenoproteins and selenoproteomes journal November 2009
Sulfur and Selenium: The Role of Oxidation State in Protein Structure and Function journal October 2003
The prokaryotic selenoproteome journal May 2004
Methionine Sulfoxide Reduction in Mammals: Characterization of Methionine- R -Sulfoxide Reductases journal March 2004
Essential Role of Selenium in the Catalytic Activities of Mammalian Thioredoxin Reductase Revealed by Characterization of Recombinant Enzymes with Selenocysteine Mutations journal April 2000
Substitution of the Thioredoxin System for Glutathione Reductase in Drosophila melanogaster journal January 2001
Reduced reliance on the trace element selenium during evolution of mammals journal January 2008
Selenocysteine in Thiol/Disulfide-Like Exchange Reactions journal May 2013
Selenocysteine Confers Resistance to Inactivation by Oxidation in Thioredoxin Reductase: Comparison of Selenium and Sulfur Enzymes journal July 2013
Substituting selenocysteine for active site cysteine 149 of phosphorylating glyceraldehyde 3-phosphate dehydrogenase reveals a peroxidase activity journal November 1998
Glyceraldehyde 3-Phosphate Dehydrogenase is Unlikely to Mediate Hydrogen Peroxide Signaling: Studies with a Novel Anti-Dimedone Sulfenic Acid Antibody journal January 2011
Structure and Redox Chemistry of Analogous Nickel Thiolato and Selenolato Complexes: Implications for the Nickel Sites in Hydrogenases journal October 1994
Reaction of alanine-3-sulfinic acid with 2-mercaptoethanol journal August 1979
ATP-dependent reduction of cysteine–sulphinic acid by S. cerevisiae sulphiredoxin journal October 2003
Kinetic studies on the peroxidase activity of selenosubtilisin journal April 1993
Exploiting the 21st amino acid—purifying and labeling proteins by selenolate targeting journal September 2004
Rapid Cross-Metathesis for Reversible Protein Modifications via Chemical Access to Se -Allyl-selenocysteine in Proteins journal August 2013
Strategic Use of Non-Native Diselenide Bridges to Steer Oxidative Protein Folding journal March 2012
Reagentless Oxidative Folding of Disulfide-Rich Peptides Catalyzed by an Intramolecular Diselenide journal March 2012
The disulfide-coupled folding pathway of apamin as derived from diselenide-quenched analogs and intermediates journal January 1999
Preparation of Selenoinsulin as a Long-Lasting Insulin Analogue journal April 2017
Gene for a novel tRNA species that accepts L-serine and cotranslationally inserts selenocysteine journal February 1988
Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase journal August 2004
Structural insights into RNA-dependent eukaryal and archaeal selenocysteine formation journal December 2007
Mutations Disrupting Selenocysteine Formation Cause Progressive Cerebello-Cerebral Atrophy journal October 2010
Interaction of a selenocysteine-incorporating tRNA with elongation factor Tu from E.coli journal January 1990
Computational identification of the selenocysteine tRNA (tRNASec) in genomes journal February 2017
Coding from a distance: dissection of the mRNA determinants required for the incorporation of selenocysteine into protein. journal October 1992
Domain Structure of the Prokaryotic Selenocysteine-specific Elongation Factor SelB journal October 1996
Recognition of the mRNA selenocysteine insertion sequence by the specialized translational elongation factor SELB. journal February 1994
Functional characterization of the eukaryotic SECIS elements which direct selenocysteine insertion at UGA codons. journal August 1993
Barriers to heterologous expression of a selenoprotein gene in bacteria. journal February 1997
Accessing human selenoproteins through chemical protein synthesis journal January 2017
Using chemical approaches to study selenoproteins—Focus on thioredoxin reductases journal November 2009
Rewiring Translation for Elongation Factor Tu-Dependent Selenocysteine Incorporation journal November 2012
Evolving tRNASec for Efficient Canonical Incorporation of Selenocysteine journal December 2014
A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro journal July 2015
Genomically Recoded Organisms Expand Biological Functions journal October 2013
Efficient Expression of Glutathione Peroxidase with Chimeric tRNA in Amber-less Escherichia coli journal August 2017
Thermodynamic and Kinetic Framework of Selenocysteyl-tRNASec Recognition by Elongation Factor SelB journal November 2009
The long extra arms of human tRNA(Ser)Sec and tRNASer function as major identity elements for serylation in an orientation-dependent, but not sequence-specific manner journal January 1993
Contributions of discrete tRNA Ser domains to aminoacylation by E.coli seryl-tRNA synthetase: a kinetic analysis using model RNA substrates journal January 1993
Escherichia coli Seryl-tRNA Synthetase Recognizes tRNASer by Its Characteristics Tertiary Structure journal February 1994
Conversion of aminoacylation specificity from tRNATyr to tRNASer in vitro journal January 1990
Tertiary structure of bacterial selenocysteine tRNA journal May 2013
Crystal structure of human selenocysteine tRNA journal August 2009
The 2.9 A crystal structure of T. thermophilus seryl-tRNA synthetase complexed with tRNA(Ser) journal March 1994
SerRS-tRNA Sec complex structures reveal mechanism of the first step in selenocysteine biosynthesis journal October 2015
Variant minihelix RNAs reveal sequence-specific recognition of the helical tRNA(Ser) acceptor stem by E.coli seryl-tRNA synthetase. journal June 1996
Recognition between tRNA Ser and archaeal seryl-tRNA synthetases monitored by suppression of bacterial amber mutations journal May 2009
tRNADB-CE 2011: tRNA gene database curated manually by experts journal November 2010
Transfer RNAs with novel cloverleaf structures journal October 2016
Insights into substrate promiscuity of human seryl-tRNA synthetase journal August 2017
Selenocysteine inserting tRNAs: an overview journal June 1999
Evolution of the selenoproteome in Helicobacter pylori and Epsilonproteobacteria journal September 2015
Facile Recoding of Selenocysteine in Nature journal March 2016
Decameric SelA•tRNASec Ring Structure Reveals Mechanism of Bacterial Selenocysteine Formation journal April 2013
The pathway to GTPase activation of elongation factor SelB on the ribosome journal November 2016
Antideterminants present in minihelix(Sec) hinder its recognition by prokaryotic elongation factor Tu. journal February 1996
The crystal structure of Cys-tRNACys–EF-Tu–GDPNP reveals general and specific features in the ternary complex and in tRNA journal February 1999
Understanding the Sequence Specificity of tRNA Binding to Elongation Factor Tu using tRNA Mutagenesis journal March 2009
Selenocysteine tRNA-specific elongation factor SelB is a structural chimaera of elongation and initiation factors journal December 2004
Engineering the elongation factor Tu for efficient selenoprotein synthesis journal July 2014
Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein journal November 1989
Complete kinetic mechanism of elongation factor Tu-dependent binding of aminoacyl-tRNA to the A site of the E.coli ribosome journal December 1998
Kinetics of the Interaction of Translation Factor SelB from Escherichia coli with Guanosine Nucleotides and Selenocysteine Insertion Sequence RNA journal April 2000
Crystal structure of an mRNA-binding fragment of Moorella thermoacetica elongation factor SelB journal August 2002
Crystal structure of the full-length bacterial selenocysteine-specific elongation factor SelB journal August 2015
Crystal structure of active elongation factor Tu reveals major domain rearrangements journal September 1993
The Bulged Nucleotide in the Escherichia coli Minimal Selenocysteine Insertion Sequence Participates in Interaction with SelB: a Genetic Approach journal November 2000
The nature of the minimal 'selenocysteine insertion sequence' (SECIS) in Escherichia coli journal February 1998
Structural basis for mRNA recognition by elongation factor SelB journal January 2005
Structural Insight into a Molecular Switch in Tandem Winged-helix Motifs from Elongation Factor SelB journal July 2007
Ribosome dynamics during decoding journal March 2017
Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S RNA journal July 1988
Selection of tRNA by the Ribosome Requires a Transition from an Open to a Closed Form journal November 2002
The Crystal Structure of the Ribosome Bound to EF-Tu and Aminoacyl-tRNA journal October 2009
The Ribosomal Stalk Binds to Translation Factors IF2, EF-Tu, EF-G and RF3 via a Conserved Region of the L12 C-terminal Domain journal January 2007
GTPase Activation of Elongation Factors Tu and G on the Ribosome journal October 2002
Interaction of Helix D of Elongation Factor Tu with Helices 4 and 5 of Protein L7/12 on the Ribosome journal March 2004
Structural Basis for the Function of the Ribosomal L7/12 Stalk in Factor Binding and GTPase Activation journal July 2005