13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica
Abstract
We report volatile fatty acids (VFAs) are an inexpensive and renewable carbon source that can be generated from gas fermentation and anaerobic digestion of fermentable wastes. The oleaginous yeast Yarrowia lipolytica is a promising biocatalyst that can utilize VFAs and convert them into triacylglycerides (TAGs). However, currently there is limited knowledge on the metabolism of Y. lipolytica when cultured on VFAs. To develop a better understanding, we used acetate as the sole carbon source to culture two strains, a control strain and a previously engineered strain for lipid overaccumulation. For both strains, metabolism during the growth phase and lipid production phase were investigated by metabolic flux analysis using two parallel sodium acetate tracers. The resolved flux distributions demonstrate that the glyoxylate shunt pathway is constantly active and the flux through gluconeogenesis varies depending on strain and phase. In particular, by regulating the activities of malate transport and pyruvate kinase, the cells divert only a portion of the glyoxylate shunt flux required to satisfy the needs for anaplerotic reactions and NADPH production through gluconeogenesis and the oxidative pentose phosphate pathway (PPP). Excess flux flows back to the tricarboxylic acid (TCA) cycle for energy production. As with the case of glucose asmore »
- Authors:
-
- Massachusetts Institute of Technology (MIT), Cambridge, MA (United States)
- Publication Date:
- Research Org.:
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1534386
- Alternate Identifier(s):
- OSTI ID: 1396975
- Grant/Contract Number:
- SC0008744
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Metabolic Engineering
- Additional Journal Information:
- Journal Volume: 38; Journal Issue: C; Journal ID: ISSN 1096-7176
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Yarrowa lipolytica; 13C metabolic flux analysis; acetate metabolism; Biodiesel; NADPH; gluconeogenesis
Citation Formats
Liu, Nian, Qiao, Kangjian, and Stephanopoulos, Gregory. 13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica. United States: N. p., 2016.
Web. doi:10.1016/j.ymben.2016.06.006.
Liu, Nian, Qiao, Kangjian, & Stephanopoulos, Gregory. 13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica. United States. https://doi.org/10.1016/j.ymben.2016.06.006
Liu, Nian, Qiao, Kangjian, and Stephanopoulos, Gregory. 2016.
"13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica". United States. https://doi.org/10.1016/j.ymben.2016.06.006. https://www.osti.gov/servlets/purl/1534386.
@article{osti_1534386,
title = {13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica},
author = {Liu, Nian and Qiao, Kangjian and Stephanopoulos, Gregory},
abstractNote = {We report volatile fatty acids (VFAs) are an inexpensive and renewable carbon source that can be generated from gas fermentation and anaerobic digestion of fermentable wastes. The oleaginous yeast Yarrowia lipolytica is a promising biocatalyst that can utilize VFAs and convert them into triacylglycerides (TAGs). However, currently there is limited knowledge on the metabolism of Y. lipolytica when cultured on VFAs. To develop a better understanding, we used acetate as the sole carbon source to culture two strains, a control strain and a previously engineered strain for lipid overaccumulation. For both strains, metabolism during the growth phase and lipid production phase were investigated by metabolic flux analysis using two parallel sodium acetate tracers. The resolved flux distributions demonstrate that the glyoxylate shunt pathway is constantly active and the flux through gluconeogenesis varies depending on strain and phase. In particular, by regulating the activities of malate transport and pyruvate kinase, the cells divert only a portion of the glyoxylate shunt flux required to satisfy the needs for anaplerotic reactions and NADPH production through gluconeogenesis and the oxidative pentose phosphate pathway (PPP). Excess flux flows back to the tricarboxylic acid (TCA) cycle for energy production. As with the case of glucose as the substrate, the primary source for lipogenic NADPH is derived from the oxidative PPP.},
doi = {10.1016/j.ymben.2016.06.006},
url = {https://www.osti.gov/biblio/1534386},
journal = {Metabolic Engineering},
issn = {1096-7176},
number = C,
volume = 38,
place = {United States},
year = {Tue Jul 05 00:00:00 EDT 2016},
month = {Tue Jul 05 00:00:00 EDT 2016}
}
Web of Science
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