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Title: 13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica

Abstract

We report volatile fatty acids (VFAs) are an inexpensive and renewable carbon source that can be generated from gas fermentation and anaerobic digestion of fermentable wastes. The oleaginous yeast Yarrowia lipolytica is a promising biocatalyst that can utilize VFAs and convert them into triacylglycerides (TAGs). However, currently there is limited knowledge on the metabolism of Y. lipolytica when cultured on VFAs. To develop a better understanding, we used acetate as the sole carbon source to culture two strains, a control strain and a previously engineered strain for lipid overaccumulation. For both strains, metabolism during the growth phase and lipid production phase were investigated by metabolic flux analysis using two parallel sodium acetate tracers. The resolved flux distributions demonstrate that the glyoxylate shunt pathway is constantly active and the flux through gluconeogenesis varies depending on strain and phase. In particular, by regulating the activities of malate transport and pyruvate kinase, the cells divert only a portion of the glyoxylate shunt flux required to satisfy the needs for anaplerotic reactions and NADPH production through gluconeogenesis and the oxidative pentose phosphate pathway (PPP). Excess flux flows back to the tricarboxylic acid (TCA) cycle for energy production. As with the case of glucose asmore » the substrate, the primary source for lipogenic NADPH is derived from the oxidative PPP.« less

Authors:
 [1];  [1];  [1]
  1. Massachusetts Institute of Technology (MIT), Cambridge, MA (United States)
Publication Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1534386
Alternate Identifier(s):
OSTI ID: 1396975
Grant/Contract Number:  
SC0008744
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Metabolic Engineering
Additional Journal Information:
Journal Volume: 38; Journal Issue: C; Journal ID: ISSN 1096-7176
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Yarrowa lipolytica; 13C metabolic flux analysis; acetate metabolism; Biodiesel; NADPH; gluconeogenesis

Citation Formats

Liu, Nian, Qiao, Kangjian, and Stephanopoulos, Gregory. 13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica. United States: N. p., 2016. Web. doi:10.1016/j.ymben.2016.06.006.
Liu, Nian, Qiao, Kangjian, & Stephanopoulos, Gregory. 13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica. United States. https://doi.org/10.1016/j.ymben.2016.06.006
Liu, Nian, Qiao, Kangjian, and Stephanopoulos, Gregory. 2016. "13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica". United States. https://doi.org/10.1016/j.ymben.2016.06.006. https://www.osti.gov/servlets/purl/1534386.
@article{osti_1534386,
title = {13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica},
author = {Liu, Nian and Qiao, Kangjian and Stephanopoulos, Gregory},
abstractNote = {We report volatile fatty acids (VFAs) are an inexpensive and renewable carbon source that can be generated from gas fermentation and anaerobic digestion of fermentable wastes. The oleaginous yeast Yarrowia lipolytica is a promising biocatalyst that can utilize VFAs and convert them into triacylglycerides (TAGs). However, currently there is limited knowledge on the metabolism of Y. lipolytica when cultured on VFAs. To develop a better understanding, we used acetate as the sole carbon source to culture two strains, a control strain and a previously engineered strain for lipid overaccumulation. For both strains, metabolism during the growth phase and lipid production phase were investigated by metabolic flux analysis using two parallel sodium acetate tracers. The resolved flux distributions demonstrate that the glyoxylate shunt pathway is constantly active and the flux through gluconeogenesis varies depending on strain and phase. In particular, by regulating the activities of malate transport and pyruvate kinase, the cells divert only a portion of the glyoxylate shunt flux required to satisfy the needs for anaplerotic reactions and NADPH production through gluconeogenesis and the oxidative pentose phosphate pathway (PPP). Excess flux flows back to the tricarboxylic acid (TCA) cycle for energy production. As with the case of glucose as the substrate, the primary source for lipogenic NADPH is derived from the oxidative PPP.},
doi = {10.1016/j.ymben.2016.06.006},
url = {https://www.osti.gov/biblio/1534386}, journal = {Metabolic Engineering},
issn = {1096-7176},
number = C,
volume = 38,
place = {United States},
year = {Tue Jul 05 00:00:00 EDT 2016},
month = {Tue Jul 05 00:00:00 EDT 2016}
}

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Cited by: 49 works
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Works referencing / citing this record:

Alternative Substrate Metabolism in Yarrowia lipolytica
journal, May 2018