skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production

Abstract

In this study, strains of Yarrowia lipolytica were engineered to express the poly-3-hydroxybutyrate (PHB) biosynthetic pathway. The genes for β-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase, and PHB synthase were cloned and inserted into the chromosome of Y. lipolytica. In shake flasks, the engineered strain accumulated PHB to 1.50 and 3.84% of cell dry weight in complex medium supplemented with glucose and acetate as carbon source, respectively. In fed-batch fermentation using acetate as sole carbon source, 7.35 g/l PHB (10.2% of cell dry weight) was produced. Selection of Y. lipolytica as host for PHB synthesis was motivated by the fact that this organism is a good lipids producer, which suggests robust acetyl-CoA supply also the precursor of the PHB pathway. Lastly, acetic acid could be supplied by gas fermentation, anaerobic digestion, and other low-cost supply route.

Authors:
 [1];  [2];  [2];  [2]
  1. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Beijing Univ. of Chemical Technology (China)
  2. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Publication Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1533314
Grant/Contract Number:  
SC0008744
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Industrial Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 44; Journal Issue: 4-5; Journal ID: ISSN 1367-5435
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Poly-3-hydroxybutyrate; PHB; Metabolic engineering; phaCAB; Acetate; Yarrowia lipolytica

Citation Formats

Li, Zheng-Jun, Qiao, Kangjian, Liu, Nian, and Stephanopoulos, Gregory. Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production. United States: N. p., 2016. Web. doi:10.1007/s10295-016-1864-1.
Li, Zheng-Jun, Qiao, Kangjian, Liu, Nian, & Stephanopoulos, Gregory. Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production. United States. doi:10.1007/s10295-016-1864-1.
Li, Zheng-Jun, Qiao, Kangjian, Liu, Nian, and Stephanopoulos, Gregory. Tue . "Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production". United States. doi:10.1007/s10295-016-1864-1. https://www.osti.gov/servlets/purl/1533314.
@article{osti_1533314,
title = {Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production},
author = {Li, Zheng-Jun and Qiao, Kangjian and Liu, Nian and Stephanopoulos, Gregory},
abstractNote = {In this study, strains of Yarrowia lipolytica were engineered to express the poly-3-hydroxybutyrate (PHB) biosynthetic pathway. The genes for β-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase, and PHB synthase were cloned and inserted into the chromosome of Y. lipolytica. In shake flasks, the engineered strain accumulated PHB to 1.50 and 3.84% of cell dry weight in complex medium supplemented with glucose and acetate as carbon source, respectively. In fed-batch fermentation using acetate as sole carbon source, 7.35 g/l PHB (10.2% of cell dry weight) was produced. Selection of Y. lipolytica as host for PHB synthesis was motivated by the fact that this organism is a good lipids producer, which suggests robust acetyl-CoA supply also the precursor of the PHB pathway. Lastly, acetic acid could be supplied by gas fermentation, anaerobic digestion, and other low-cost supply route.},
doi = {10.1007/s10295-016-1864-1},
journal = {Journal of Industrial Microbiology and Biotechnology},
issn = {1367-5435},
number = 4-5,
volume = 44,
place = {United States},
year = {2016},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 10 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Biosynthesis of poly(3-hydroxybutyrate- co -3-hydroxyalkanoates) by recombinant bacteria expressing the PHA synthase gene phaC1 from Pseudomonas sp. 61-3
journal, April 2000

  • Matsusaki, H.; Abe, H.; Taguchi, K.
  • Applied Microbiology and Biotechnology, Vol. 53, Issue 4
  • DOI: 10.1007/s002530051633

Engineering of acetyl-CoA metabolism for the improved production of polyhydroxybutyrate in Saccharomyces cerevisiae
journal, January 2012


Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains
journal, July 2014


Anaerobic CO 2 fixation by the acetogenic bacterium Moorella thermoacetica
journal, May 2013

  • Hu, Peng; Rismani-Yazdi, Hamid; Stephanopoulos, Gregory
  • AIChE Journal, Vol. 59, Issue 9
  • DOI: 10.1002/aic.14127

PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo
journal, January 1997

  • Sim, Sang Jun; Snell, Kristi D.; Hogan, Scott A.
  • Nature Biotechnology, Vol. 15, Issue 1
  • DOI: 10.1038/nbt0197-63

Effects of chromosomal gene copy number and locations on polyhydroxyalkanoate synthesis by Escherichia coli and Halomonas sp.
journal, March 2015

  • Yin, Jin; Wang, Huan; Fu, Xiao-Zhi
  • Applied Microbiology and Biotechnology, Vol. 99, Issue 13
  • DOI: 10.1007/s00253-015-6510-8

Enhanced production of polyhydroxybutyrate by multiple dividing E. coli
journal, July 2016


Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica
journal, July 2013

  • Xue, Zhixiong; Sharpe, Pamela L.; Hong, Seung-Pyo
  • Nature Biotechnology, Vol. 31, Issue 8
  • DOI: 10.1038/nbt.2622

Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica
journal, May 2015


Improved polyhydroxybutyrate production by Saccharomyces cerevisiae through the use of the phosphoketolase pathway
journal, March 2013

  • Kocharin, Kanokarn; Siewers, Verena; Nielsen, Jens
  • Biotechnology and Bioengineering, Vol. 110, Issue 8
  • DOI: 10.1002/bit.24888

Volatile fatty acids production from food waste: Effects of pH, temperature, and organic loading rate
journal, September 2013


A biochemical model describing volatile fatty acid metabolism in thermophilic aerobic digestion of wastewater sludge
journal, August 1996


Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants — A review
journal, March 2007


A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme
journal, October 2008

  • Taguchi, S.; Yamada, M.; Matsumoto, K.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 45
  • DOI: 10.1073/pnas.0805653105

Polyhydroxyalkanoates: biodegradable polymers with a range of applications
journal, January 2007

  • Philip, S.; Keshavarz, T.; Roy, I.
  • Journal of Chemical Technology & Biotechnology, Vol. 82, Issue 3
  • DOI: 10.1002/jctb.1667

Saccharomyces cerevisiae expressing bacterial polyhydroxybutyrate synthase produces poly-3-hydroxybutyrate
journal, May 1996


Effects of recombinant precursor pathway variations on poly[(R)-3-hydroxybutyrate] synthesis in Saccharomyces cerevisiae
journal, July 2006


Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose
journal, February 2015

  • Sandström, Anders G.; Muñoz de las Heras, Alejandro; Portugal-Nunes, Diogo
  • AMB Express, Vol. 5, Issue 1
  • DOI: 10.1186/s13568-015-0100-0

Engineering the bacterial shapes for enhanced inclusion bodies accumulation
journal, May 2015


Engineering the growth pattern and cell morphology for enhanced PHB production by Escherichia coli
journal, July 2016

  • Wu, Hong; Chen, Jinchun; Chen, Guo-Qiang
  • Applied Microbiology and Biotechnology, Vol. 100, Issue 23
  • DOI: 10.1007/s00253-016-7715-1