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Title: Systematic comparison of co-expression of multiple recombinant thermophilic enzymes in Escherichia coli BL21(DE3)

Abstract

Not provided.

Authors:
; ;
Publication Date:
Research Org.:
Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1533279
DOE Contract Number:  
EE0006968
Resource Type:
Journal Article
Journal Name:
Applied Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 101; Journal Issue: 11; Journal ID: ISSN 0175-7598
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
Biotechnology & Applied Microbiology

Citation Formats

Chen, Hui, Huang, Rui, and Zhang, Y. -H. Percival. Systematic comparison of co-expression of multiple recombinant thermophilic enzymes in Escherichia coli BL21(DE3). United States: N. p., 2017. Web. doi:10.1007/s00253-017-8206-8.
Chen, Hui, Huang, Rui, & Zhang, Y. -H. Percival. Systematic comparison of co-expression of multiple recombinant thermophilic enzymes in Escherichia coli BL21(DE3). United States. doi:10.1007/s00253-017-8206-8.
Chen, Hui, Huang, Rui, and Zhang, Y. -H. Percival. Wed . "Systematic comparison of co-expression of multiple recombinant thermophilic enzymes in Escherichia coli BL21(DE3)". United States. doi:10.1007/s00253-017-8206-8.
@article{osti_1533279,
title = {Systematic comparison of co-expression of multiple recombinant thermophilic enzymes in Escherichia coli BL21(DE3)},
author = {Chen, Hui and Huang, Rui and Zhang, Y. -H. Percival},
abstractNote = {Not provided.},
doi = {10.1007/s00253-017-8206-8},
journal = {Applied Microbiology and Biotechnology},
issn = {0175-7598},
number = 11,
volume = 101,
place = {United States},
year = {2017},
month = {3}
}

Works referenced in this record:

A Facile Method for High-throughput Co-expression of Protein Pairs
journal, July 2004

  • Alexandrov, Andrei; Vignali, Marissa; LaCount, Douglas J.
  • Molecular & Cellular Proteomics, Vol. 3, Issue 9
  • DOI: 10.1074/mcp.T400008-MCP200

Modular Chemoenzymatic One-Pot Syntheses in Aqueous Media: Combination of a Palladium-Catalyzed Cross-Coupling with an Asymmetric Biotransformation
journal, November 2008

  • Burda, Edyta; Hummel, Werner; Gröger, Harald
  • Angewandte Chemie International Edition, Vol. 47, Issue 49
  • DOI: 10.1002/anie.200801341

High yield purification of a tagged cytoplasmic [NiFe]-hydrogenase and a catalytically-active nickel-free intermediate form
journal, March 2015

  • Chandrayan, Sanjeev K.; Wu, Chang-Hao; McTernan, Patrick M.
  • Protein Expression and Purification, Vol. 107
  • DOI: 10.1016/j.pep.2014.10.018

Multienzymatic synthesis of nucleic acid derivatives: a general perspective
journal, May 2015


Biosynthesis of natural products by microbial iterative hybrid PKS–NRPS
journal, January 2013


Disparity between changes in mRNA abundance and enzyme activity in Corynebacterium glutamicum: implications for DNA microarray analysis
journal, December 2002

  • Glanemann, C.; Loos, A.; Gorret, N.
  • Applied Microbiology and Biotechnology, Vol. 61, Issue 1
  • DOI: 10.1007/s00253-002-1191-5

Calnexin co-expression and the use of weaker promoters increase the expression of correctly assembled Shaker potassium channel in insect cells
journal, February 2003

  • Higgins, Matthew K.; Demir, Mukerrem; Tate, Christopher G.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1610, Issue 1
  • DOI: 10.1016/S0005-2736(02)00715-0

Construction of a tunable multi-enzyme-coordinate expression system for biosynthesis of chiral drug intermediates
journal, July 2016

  • Jiang, Wei; Fang, Baishan
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep30462

Coexpression of Proteins in Bacteria Using T7-Based Expression Plasmids: Expression of Heteromeric Cell-Cycle and Transcriptional Regulatory Complexes
journal, December 2000

  • Johnston, Karen; Clements, Adrienne; Venkataramani, Ravichandran N.
  • Protein Expression and Purification, Vol. 20, Issue 3
  • DOI: 10.1006/prep.2000.1313

Low-Copy Plasmids can Perform as Well as or Better Than High-Copy Plasmids for Metabolic Engineering of Bacteria
journal, October 2000

  • Jones, Kristala L.; Kim, Seon-Won; Keasling, J. D.
  • Metabolic Engineering, Vol. 2, Issue 4
  • DOI: 10.1006/mben.2000.0161

Two-promoter vector is highly efficient for overproduction of protein complexes
journal, June 2004

  • Kim, Kyung-Jin; Kim, Han-Eol; Lee, Kwang-Hoon
  • Protein Science, Vol. 13, Issue 6
  • DOI: 10.1110/ps.04644504

Rare codon clusters at 5′-end influence heterologous expression of archaeal gene in Escherichia coli
journal, November 2006


Multi-enzymatic synthesis
journal, April 2010

  • Lopez-Gallego, Fernando; Schmidt-Dannert, Claudia
  • Current Opinion in Chemical Biology, Vol. 14, Issue 2
  • DOI: 10.1016/j.cbpa.2009.11.023

Enhanced production of coenzyme Q 10 by self-regulating the engineered MEP pathway in Rhodobacter sphaeroides : Self-Regulation of Metabolic Pathway
journal, October 2013

  • Lu, Wenqiang; Ye, Lidan; Xu, Haoming
  • Biotechnology and Bioengineering, Vol. 111, Issue 4
  • DOI: 10.1002/bit.25130

Significantly enhanced production of isoprene by ordered coexpression of genes dxs, dxr, and idi in Escherichia coli
journal, November 2012

  • Lv, Xiaomei; Xu, Haoming; Yu, Hongwei
  • Applied Microbiology and Biotechnology, Vol. 97, Issue 6
  • DOI: 10.1007/s00253-012-4485-2

Comparing the utility of β-glucuronidase and green fluorescent protein for detection of weak promoter activity inArabidopsis thaliana
journal, December 2000

  • Mantis, Joanna; Tague, Brian W.
  • Plant Molecular Biology Reporter, Vol. 18, Issue 4
  • DOI: 10.1007/BF02825059

In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose
journal, July 2014


Genomic and Coexpression Analyses Predict Multiple Genes Involved in Triterpene Saponin Biosynthesis in Medicago truncatula
journal, March 2010

  • Naoumkina, Marina A.; Modolo, Luzia V.; Huhman, David V.
  • The Plant Cell, Vol. 22, Issue 3
  • DOI: 10.1105/tpc.109.073270

Mammalian AMP-activated protein kinase: functional, heterotrimeric complexes by co-expression of subunits in Escherichia coli
journal, August 2003


Assembly and multiple gene expression of thermophilic enzymes in Escherichia coli for in vitro metabolic engineering : Multiple Expression of Thermophilic Enzymes
journal, September 2014

  • Ninh, Pham Huynh; Honda, Kohsuke; Sakai, Takaaki
  • Biotechnology and Bioengineering, Vol. 112, Issue 1
  • DOI: 10.1002/bit.25338

Post-transcriptional control by global regulators of gene expression in bacteria
journal, April 2000


A synthetic biochemistry module for production of bio-based chemicals from glucose
journal, April 2016

  • Opgenorth, Paul H.; Korman, Tyler P.; Bowie, James U.
  • Nature Chemical Biology, Vol. 12, Issue 6
  • DOI: 10.1038/nchembio.2062

Whole-Cell One-Pot Biosynthesis of Azelaic Acid
journal, December 2013


Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies
journal, September 2006

  • Romier, Christophe; Ben Jelloul, Marouane; Albeck, Shira
  • Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 10
  • DOI: 10.1107/S0907444906031003

Automated design of synthetic ribosome binding sites to control protein expression
journal, October 2009

  • Salis, Howard M.; Mirsky, Ethan A.; Voigt, Christopher A.
  • Nature Biotechnology, Vol. 27, Issue 10, p. 946-950
  • DOI: 10.1038/nbt.1568

Multienzyme-Catalyzed Processes: Next-Generation Biocatalysis
journal, January 2011

  • Santacoloma, Paloma A.; Sin, Gürkan; Gernaey, Krist V.
  • Organic Process Research & Development, Vol. 15, Issue 1
  • DOI: 10.1021/op1002159

Advanced genetic strategies for recombinant protein expression in Escherichia coli
journal, January 2005


Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production
journal, May 2010


A Modular Polycistronic Expression System for Overexpressing Protein Complexes in Escherichia coli
journal, February 2001


Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells
journal, July 2010


Strategies for protein coexpression in Escherichia coli
journal, January 2006


Synthesis of Proteins in Escherichia coli is Limited by the Concentration of Free Ribosomes
journal, June 1993

  • Vind, Jesper; Sørensen, Michael A.; Rasmussen, Michael D.
  • Journal of Molecular Biology, Vol. 231, Issue 3
  • DOI: 10.1006/jmbi.1993.1319

Cloning of the Gene for Inorganic Pyrophosphatase from a Thermoacidophilic Archaeon, Sulfolobus sp. Strain 7, and Overproduction of the Enzyme by Coexpression of tRNA for Arginine Rare Codon
journal, January 1998

  • Wakagi, Takayoshi; Oshima, Tairo; Imamura, Hiromi
  • Bioscience, Biotechnology, and Biochemistry, Vol. 62, Issue 12
  • DOI: 10.1271/bbb.62.2408

Biohydrogenation from Biomass Sugar Mediated by In Vitro Synthetic Enzymatic Pathways
journal, March 2011


Heterologous Expression of a Myxobacterial Natural Products Assembly Line in Pseudomonads via Red/ET Recombineering
journal, March 2005


Heterologous Expression, Purification, and Characterization of a Highly Active Xylose Reductase from Neurospora crassa
journal, March 2005


Simple Cloning via Direct Transformation of PCR Product (DNA Multimer) to Escherichia coli and Bacillus subtilis
journal, December 2011

  • You, Chun; Zhang, Xiao-Zhou; Zhang, Y. -H. Percival
  • Applied and Environmental Microbiology, Vol. 78, Issue 5
  • DOI: 10.1128/AEM.07105-11

Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering
journal, May 2009

  • Zha, Wenjuan; Rubin-Pitel, Sheryl B.; Shao, Zengyi
  • Metabolic Engineering, Vol. 11, Issue 3, p. 192-198
  • DOI: 10.1016/j.ymben.2009.01.005

Production of biofuels and biochemicals by in vitro synthetic biosystems: Opportunities and challenges
journal, November 2015


Regulation of Cellulase Synthesis in Batch and Continuous Cultures of Clostridium thermocellum
journal, December 2004


One-Pot Biosynthesis of High-Concentration α-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes
journal, February 2016

  • Zhou, Wei; You, Chun; Ma, Hongwu
  • Journal of Agricultural and Food Chemistry, Vol. 64, Issue 8
  • DOI: 10.1021/acs.jafc.5b05648

Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production
journal, February 2015

  • Zhu, Yueming; Li, Hongyi; Liu, Pingping
  • Journal of Industrial Microbiology & Biotechnology, Vol. 42, Issue 5
  • DOI: 10.1007/s10295-014-1578-1

Deep oxidation of glucose in enzymatic fuel cells through a synthetic enzymatic pathway containing a cascade of two thermostable dehydrogenases
journal, June 2012


A high-energy-density sugar biobattery based on a synthetic enzymatic pathway
journal, January 2014

  • Zhu, Zhiguang; Kin Tam, Tsz; Sun, Fangfang
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms4026