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Title: High-Throughput Screening of Catalytic H2 Production

Journal Article · · Angewandte Chemie (International Edition)
 [1];  [1];  [1];  [1];  [1]
  1. Stanford Univ., CA (United States)
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Hydrogenases, ferredoxins, and ferredoxin-NADP+ reductases (FNR) are redox proteins that mediate electron metabolism in vivo, and are also potential components for biological H2 production technologies. A high-throughput H2 production assay device (H2PAD) is presented that enables simultaneous evaluation of 96 individual H2 production reactions to identify components that improve performance. Using a CCD camera and image analysis software, H2PAD senses the chemo-optical response of Pd/WO3 thin films to the H2 produced. H2PAD-enabled discovery of hydrogenase and FNR mutants that enhance biological H2 production is reported. From a library of 10 080 randomly mutated Clostridium pasteurianum [FeFe] hydrogenases, we found a mutant with nearly 3-fold higher H2 production specific activity. From a library of 400 semi-randomly mutated Oryza sativa FNR, the top hit enabled a 60 % increase in NADPH-driven H2 production rates. H2PAD can also facilitate elucidation of fundamental biochemical mechanisms within these systems.

Research Organization:
Stanford Univ., CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
SC0002010
OSTI ID:
1533092
Journal Information:
Angewandte Chemie (International Edition), Vol. 56, Issue 4; ISSN 1433-7851
Publisher:
WileyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 11 works
Citation information provided by
Web of Science

References (25)

Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii journal July 2004
Differential Interaction of Maize Root Ferredoxin:NADP + Oxidoreductase with Photosynthetic and Non-Photosynthetic Ferredoxin Isoproteins journal July 2000
Photocatalytic Hydrogen Evolution with a Hydrogenase in a Mediator-Free System under High Levels of Oxygen journal September 2013
Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii journal February 2005
High-Throughput Screening System for Catalytic Hydrogen-Producing Materials journal January 2002
High-throughput biosensor discriminates between different algal H 2 -photoproducing strains : Biosensor for Algal Hydrogen Photoproduction journal February 2014
Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk method journal July 1961
Enhanced oxygen tolerance of hydrogenase from Klebsiella oxytoca HP1 by Gly–Cys exchanges nearby Fe–S clusters as biocatalysts in biofuel cells or hydrogen production journal October 2014
Discovery of Two Novel Radical S -Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase journal April 2004
Design of a new biosensor for algal H2 production based on the H2-sensing system of Rhodobacter capsulatus journal August 2011
High-Throughput Screening Assay for Biological Hydrogen Production journal June 2008
Development of a synthetic pathway to convert glucose to hydrogen using cell free extracts journal August 2015
Generation of hydrogen from NADPH using an [FeFe] hydrogenase journal February 2012
Advances in biological hydrogen production processes journal November 2008
Characterization of [FeFe] Hydrogenase O 2 Sensitivity Using a New, Physiological Approach journal July 2016
Photocatalytic Hydrogen Evolution with a Hydrogenase in a Mediator-Free System under High Levels of Oxygen journal September 2013
Insulation of a synthetic hydrogen metabolism circuit in bacteria journal January 2010
Enzymatic production of biohydrogen: Biotechnology journal June 2000
Role of Arg100 and Arg264 from Anabaena PCC 7119 Ferredoxin−NADP + Reductase for Optimal NADP + Binding and Electron Transfer † journal December 1998
Cell-free synthesis and maturation of [FeFe] hydrogenases journal January 2008
High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway journal May 2007
Evolution of an [FeFe] hydrogenase with decreased oxygen sensitivity journal February 2012
Regeneration of adenosine triphosphate from glycolytic intermediates for cell-free protein synthesis journal January 2001
Tyrosine, Cysteine, and S-Adenosyl Methionine Stimulate In Vitro [FeFe] Hydrogenase Activation journal October 2009
Hydrogen detection based on coloration of anodic tungsten oxide film journal February 1992

Cited By (3)

Remodeling of the Photosynthetic Chain Promotes Direct CO 2 Conversion into Valuable Aromatic Compounds journal October 2018
O 2 sensitivity and H 2 production activity of hydrogenases—A review journal August 2019
Remodeling of the Photosynthetic Chain Promotes Direct CO 2 Conversion into Valuable Aromatic Compounds journal December 2018

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
chemistry
high-throughput screening
hydrogen
hydrogenase
metalloproteins
redox chemistry