Isothermal amplification of DNA
Patent
·
OSTI ID:1532093
A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.
- Research Organization:
- Harvard College, Cambridge, MA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-96ER62251
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Number(s):
- 8,709,724
- Application Number:
- 10/813,693
- OSTI ID:
- 1532093
- Country of Publication:
- United States
- Language:
- English
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