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Title: Neurotransmitter sensors and methods of using the same

Abstract

Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.

Inventors:
; ;
Publication Date:
Research Org.:
Carnegie Inst. of Washington, Washington, DC (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531646
Patent Number(s):
7,777,016
Application Number:
11/665,343
Assignee:
Carnegie Institution of Washington (Washington, DC)
DOE Contract Number:  
FG02-04ER15542
Resource Type:
Patent
Resource Relation:
Patent File Date: 2005-10-14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Okumoto, Sakiko, Looger, Loren L., and Frommer, Wolf B. Neurotransmitter sensors and methods of using the same. United States: N. p., 2010. Web.
Okumoto, Sakiko, Looger, Loren L., & Frommer, Wolf B. Neurotransmitter sensors and methods of using the same. United States.
Okumoto, Sakiko, Looger, Loren L., and Frommer, Wolf B. Tue . "Neurotransmitter sensors and methods of using the same". United States. https://www.osti.gov/servlets/purl/1531646.
@article{osti_1531646,
title = {Neurotransmitter sensors and methods of using the same},
author = {Okumoto, Sakiko and Looger, Loren L. and Frommer, Wolf B.},
abstractNote = {Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {8}
}

Patent:

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Works referenced in this record:

Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein
journal, February 1999

  • Tolosa, Leah; Gryczynski, Ignacy; Eichhorn, Lisa R.
  • Analytical Biochemistry, Vol. 267, Issue 1
  • DOI: 10.1006/abio.1998.2974

Engineered proteins for analyte sensing
patent, March 2001


Protein localization in living cells and tissues using FRET and FLIM
journal, December 2003


A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
journal, January 2002

  • Nagai, Takeharu; Ibata, Keiji; Park, Eun Sun
  • Nature Biotechnology, Vol. 20, Issue 1
  • DOI: 10.1038/nbt0102-87

Visualization of maltose uptake in living yeast cells by fluorescent nanosensors
journal, July 2002

  • Fehr, M.; Frommer, W. B.; Lalonde, S.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 15
  • DOI: 10.1073/pnas.142089199

Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
journal, August 1997

  • Miyawaki, Atsushi; Llopis, Juan; Heim, Roger
  • Nature, Vol. 388, Issue 6645
  • DOI: 10.1038/42264

Optimized Heterologous Expression of the Polymorphic Human Glutathione Transferase M1-1 Based on Silent Mutations in the Corresponding cDNA
journal, June 1996

  • Widersten, Mikael; Huang, Mao; Mannervik, Bengt
  • Protein Expression and Purification, Vol. 7, Issue 4
  • DOI: 10.1006/prep.1996.0054

Reductive, energy-transfer fluorogenic probes
patent, August 1998


Design of Bioelectronic Interfaces by Exploiting Hinge-Bending Motions in Proteins
journal, August 2001


The Genome of the Natural Genetic Engineer Agrobacterium tumefaciens C58
journal, December 2001


Fluorescent protein sensors for detection of analytes
patent, December 1999


X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins
journal, January 2004

  • Schäfer, Karsten; Magnusson, Ulrika; Scheffel, Frank
  • Journal of Molecular Biology, Vol. 335, Issue 1, p. 261-274
  • DOI: 10.1016/j.jmb.2003.10.042

Novel biosensors for the detection of estrogen receptor ligands
journal, August 2005

  • De, Siddhartha; Macara, Ian G.; Lannigan, Deborah A.
  • The Journal of Steroid Biochemistry and Molecular Biology, Vol. 96, Issue 3-4
  • DOI: 10.1016/j.jsbmb.2005.04.030

Fusion estrogen receptor proteins: toward the development of receptor-based agonists and antagonists
journal, September 2001


Kinetic and Thermodynamic Studies of Purine Repressor Binding to Corepressor and Operator DNA
journal, April 1998

  • Xu, Han; Moraitis, Markos; Reedstrom, Ross J.
  • Journal of Biological Chemistry, Vol. 273, Issue 15
  • DOI: 10.1074/jbc.273.15.8958

Shedding Light on Cell Signaling: Interpretation of FRET Biosensors
journal, January 2003


Viewpoint: Are Studies in Genetically Altered Mice Out of Control?
journal, June 2000


Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering
journal, September 2005

  • Deuschle, Karen; Okumoto, Sakiko; Fehr, Marcus
  • Protein Science, Vol. 14, Issue 9
  • DOI: 10.1110/ps.051508105

Fluorescent protein indicators
patent, October 2002


Tandem fluorescent protein constructs
patent, November 1999


Construction of a fluorescent biosensor family
journal, November 2002

  • De Lorimier, Robert M.; Smith, J. Jeff; Dwyer, Mary A.
  • Protein Science, Vol. 11, Issue 11
  • DOI: 10.1110/ps.021860

Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.
journal, December 1980

  • Gunsalus, R. P.; Yanofsky, C.
  • Proceedings of the National Academy of Sciences, Vol. 77, Issue 12
  • DOI: 10.1073/pnas.77.12.7117

Building and breeding molecules to spy on cells and tumors
journal, November 2004


Strategies for optimizing heterologous protein expression in Escherichia coli
journal, February 1998


Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering
journal, December 2001

  • Zhang, J.; Ma, Y.; Taylor, S. S.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 26
  • DOI: 10.1073/pnas.211566798

Domain dislocation: a change of core structure in periplasmic binding proteins in their evolutionary history
journal, February 1999

  • Fukami-Kobayashi, Kaoru; Tateno, Yoshio; Nishikawa, Ken
  • Journal of Molecular Biology, Vol. 286, Issue 1
  • DOI: 10.1006/jmbi.1998.2454

Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors
journal, June 2005

  • Okumoto, S.; Looger, L. L.; Micheva, K. D.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 24
  • DOI: 10.1073/pnas.0503274102

Biosensor
patent, August 2001


Periplasmic binding proteins: a versatile superfamily for protein engineering
journal, August 2004


Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein
journal, January 1996