Molecular evidence of toxic effect on the biofilm and its matrix

Ding, Yuanzhao; Zhou, Yufan; Yao, Juan; Xiong, Yijia; Zhu, Zihua; Yu, Xiao-Ying

doi:10.1039/C8AN02512F

Title: Molecular evidence of toxic effect on the biofilm and its matrix

Journal Article · Sun Apr 21 00:00:00 EDT 2019 · The Analyst

DOI:https://doi.org/10.1039/C8AN02512F· OSTI ID:1530828

Ding, Yuanzhao ^[1]; Zhou, Yufan ^[2];

^[3]; Xiong, Yijia ^[4];

^[3];

^[3]

Nanyang Technological University
UNIVERSITY PROGRAMS
BATTELLE (PACIFIC NW LAB)
Western University of Health Sciences

The biofilm and its extracellular polymeric substance (EPS) are known to respond to toxic chemicals. Shewanella oneidensis MR-1 wild-type (WT) and a hyper-adhesive mutant CP2-1-S1 (CP) are used as model organisms and Cr(VI) is selected as a toxic chemical in this study. We investigate the following the science questions: 1) what are the chemical differences revealed in molecular mapping between WT and CP; and 2) how WT and CP biofilms respond to a toxic chemical. The System for Analysis at the Liquid Vacuum Interface (SALVI) is employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). The SIMS spectra and principal component analysis (PCA) are used to study the biofilm spatial and chemical heterogeneity. In situ measurement of biofilm metabolic responses to toxic chemicals has been given for the first time. The method presented here is expected to be viable to study responses to other chemicals including antimicrobials in the context of antimicrobial resistance to biofilms with diverse applications.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1530828

Report Number(s):: PNNL-SA-135367

Journal Information:: The Analyst, Vol. 144, Issue 8

Country of Publication:: United States

Language:: English

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