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Title: Molecular evidence of toxic effect on the biofilm and its matrix

Abstract

The biofilm and its extracellular polymeric substance (EPS) are known to respond to toxic chemicals. Shewanella oneidensis MR-1 wild-type (WT) and a hyper-adhesive mutant CP2-1-S1 (CP) are used as model organisms and Cr(VI) is selected as a toxic chemical in this study. We investigate the following the science questions: 1) what are the chemical differences revealed in molecular mapping between WT and CP; and 2) how WT and CP biofilms respond to a toxic chemical. The System for Analysis at the Liquid Vacuum Interface (SALVI) is employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). The SIMS spectra and principal component analysis (PCA) are used to study the biofilm spatial and chemical heterogeneity. In situ measurement of biofilm metabolic responses to toxic chemicals has been given for the first time. The method presented here is expected to be viable to study responses to other chemicals including antimicrobials in the context of antimicrobial resistance to biofilms with diverse applications.

Authors:
 [1];  [2]; ORCiD logo [3];  [4]; ORCiD logo [3]; ORCiD logo [3]
  1. Nanyang Technological University
  2. UNIVERSITY PROGRAMS
  3. BATTELLE (PACIFIC NW LAB)
  4. Western University of Health Sciences
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1530828
Report Number(s):
PNNL-SA-135367
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
The Analyst
Additional Journal Information:
Journal Volume: 144; Journal Issue: 8
Country of Publication:
United States
Language:
English
Subject:
biofilm, Shewanella oneidensis, CP2-1-S1, SALVI, ToF-SIMS, toxicity

Citation Formats

Ding, Yuanzhao, Zhou, Yufan, Yao, Juan, Xiong, Yijia, Zhu, Zihua, and Yu, Xiao-Ying. Molecular evidence of toxic effect on the biofilm and its matrix. United States: N. p., 2019. Web. doi:10.1039/C8AN02512F.
Ding, Yuanzhao, Zhou, Yufan, Yao, Juan, Xiong, Yijia, Zhu, Zihua, & Yu, Xiao-Ying. Molecular evidence of toxic effect on the biofilm and its matrix. United States. doi:10.1039/C8AN02512F.
Ding, Yuanzhao, Zhou, Yufan, Yao, Juan, Xiong, Yijia, Zhu, Zihua, and Yu, Xiao-Ying. Sun . "Molecular evidence of toxic effect on the biofilm and its matrix". United States. doi:10.1039/C8AN02512F.
@article{osti_1530828,
title = {Molecular evidence of toxic effect on the biofilm and its matrix},
author = {Ding, Yuanzhao and Zhou, Yufan and Yao, Juan and Xiong, Yijia and Zhu, Zihua and Yu, Xiao-Ying},
abstractNote = {The biofilm and its extracellular polymeric substance (EPS) are known to respond to toxic chemicals. Shewanella oneidensis MR-1 wild-type (WT) and a hyper-adhesive mutant CP2-1-S1 (CP) are used as model organisms and Cr(VI) is selected as a toxic chemical in this study. We investigate the following the science questions: 1) what are the chemical differences revealed in molecular mapping between WT and CP; and 2) how WT and CP biofilms respond to a toxic chemical. The System for Analysis at the Liquid Vacuum Interface (SALVI) is employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). The SIMS spectra and principal component analysis (PCA) are used to study the biofilm spatial and chemical heterogeneity. In situ measurement of biofilm metabolic responses to toxic chemicals has been given for the first time. The method presented here is expected to be viable to study responses to other chemicals including antimicrobials in the context of antimicrobial resistance to biofilms with diverse applications.},
doi = {10.1039/C8AN02512F},
journal = {The Analyst},
number = 8,
volume = 144,
place = {United States},
year = {2019},
month = {4}
}