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Title: Kindlin Is Mechanosensitive: Force-Induced Conformational Switch Mediates Cross-Talk among Integrins

Abstract

Mechanical stresses directly regulate the function of several proteins of the integrin-mediated focal adhesion complex as they experience intra- and extracellular forces. Kindlin is a largely overlooked member of the focal adhesion complex whose roles in cellular mechanotransduction are only recently being identified. Recent crystallographic experiments have revealed that kindlins can form dimers that bind simultaneously to two integrins, providing a mechanistic explanation of how kindlins may promote integrin activation and clustering. In this study, using the newly identified molecular structure, we modeled the response of the kindlin2 dimer in complex with integrin β1 to mechanical cytoskeletal forces on integrins. Using molecular dynamics simulations, we show that forces on integrins are directly transmitted to the kindlin2 dimerization site, resulting in a shift in an R577-S550/E553 interaction network at this site. Under force, R577 on one protomer switches from interacting with S550 to forming new hydrogen bonds with E553 on the neighboring protomer, resulting in the strengthening of the kindlin2 dimer in complex with integrin β1. This force-induced strengthening is similar to the catch-bond mechanisms that have previously been observed in other adhesion molecules. Based on our results, we propose that the kindlin2 dimer is mechanosensitive and can strengthen integrin-mediated focalmore » adhesions under force by shifting the interactions at its dimerization sites.« less

Authors:
 [1];  [1];  [1];  [2]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering and Mechanical Engineering, Molecular Cell Biomechanics Lab.
  2. Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering and Mechanical Engineering, Molecular Cell Biomechanics Lab.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integravtive Bioimaging Division
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory-National Energy Research Scientific Computing Center (NERSC)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1530382
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Biophysical Journal
Additional Journal Information:
Journal Volume: 116; Journal Issue: 6; Journal ID: ISSN 0006-3495
Country of Publication:
United States
Language:
English

Citation Formats

Jahed, Zeinab, Haydari, Zainab, Rathish, Akshay, and Mofrad, Mohammad R. K. Kindlin Is Mechanosensitive: Force-Induced Conformational Switch Mediates Cross-Talk among Integrins. United States: N. p., 2019. Web. doi:10.1016/j.bpj.2019.01.038.
Jahed, Zeinab, Haydari, Zainab, Rathish, Akshay, & Mofrad, Mohammad R. K. Kindlin Is Mechanosensitive: Force-Induced Conformational Switch Mediates Cross-Talk among Integrins. United States. doi:10.1016/j.bpj.2019.01.038.
Jahed, Zeinab, Haydari, Zainab, Rathish, Akshay, and Mofrad, Mohammad R. K. Fri . "Kindlin Is Mechanosensitive: Force-Induced Conformational Switch Mediates Cross-Talk among Integrins". United States. doi:10.1016/j.bpj.2019.01.038.
@article{osti_1530382,
title = {Kindlin Is Mechanosensitive: Force-Induced Conformational Switch Mediates Cross-Talk among Integrins},
author = {Jahed, Zeinab and Haydari, Zainab and Rathish, Akshay and Mofrad, Mohammad R. K.},
abstractNote = {Mechanical stresses directly regulate the function of several proteins of the integrin-mediated focal adhesion complex as they experience intra- and extracellular forces. Kindlin is a largely overlooked member of the focal adhesion complex whose roles in cellular mechanotransduction are only recently being identified. Recent crystallographic experiments have revealed that kindlins can form dimers that bind simultaneously to two integrins, providing a mechanistic explanation of how kindlins may promote integrin activation and clustering. In this study, using the newly identified molecular structure, we modeled the response of the kindlin2 dimer in complex with integrin β1 to mechanical cytoskeletal forces on integrins. Using molecular dynamics simulations, we show that forces on integrins are directly transmitted to the kindlin2 dimerization site, resulting in a shift in an R577-S550/E553 interaction network at this site. Under force, R577 on one protomer switches from interacting with S550 to forming new hydrogen bonds with E553 on the neighboring protomer, resulting in the strengthening of the kindlin2 dimer in complex with integrin β1. This force-induced strengthening is similar to the catch-bond mechanisms that have previously been observed in other adhesion molecules. Based on our results, we propose that the kindlin2 dimer is mechanosensitive and can strengthen integrin-mediated focal adhesions under force by shifting the interactions at its dimerization sites.},
doi = {10.1016/j.bpj.2019.01.038},
journal = {Biophysical Journal},
issn = {0006-3495},
number = 6,
volume = 116,
place = {United States},
year = {2019},
month = {3}
}