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Title: SBIR Phase I Final Report: “Site-Specific Nitrous Oxide Isotope Analyzer for Measuring Bioremediation”

Abstract

In Phase I, LGR demonstrated technical feasibility by fabricating a prototype system for quantification of N2O, 15N14NO, 14N15NO, and 14N14N18O at ambient concentrations. The prototype was tested on a variety of gas cylinders and found to quantify [N2O], del15N, del15Nalpha, del15Nbeta, and del18O in N2O with a precision of ±0.05 ppb, ±0.4 ‰, ±0.45 ‰, ±0.6 ‰, and ±1.2 ‰ respectively (1sigma, 100s for [N2O] = 322 ppb). Measurements of calibrated and diluted gas standards demonstrated that the Phase I sensor was accurate to better than ±1ppb and ±1‰ for N2O concentration and isotope ratios, respectively, with a linear response over a wide dynamic range of [N2O] = 150 – 10000 ppb. Potential cross-interferences with ambient water vapor and carbon dioxide were identified and mitigated by using front-end sample conditioning techniques. The instrument was then deployed in conjunction with Professor Joseph von Fischer’s group in the Department of Biology at Colorado State University, where it was interfaced to a novel, collapsible Mylar chamber for continuous flow studies of soil gases with minimal pressure effects. This setup was used in conjunction with 15N-labelling experiments to identify nitrification and denitrification pathways in soil samples obtained from NSF LTER sites.

Authors:
Publication Date:
Research Org.:
Los Gatos Research
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1527162
Report Number(s):
Phase I Final Report
DOE Contract Number:  
sc0007654
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 54 ENVIRONMENTAL SCIENCES

Citation Formats

Gupta, Manish. SBIR Phase I Final Report: “Site-Specific Nitrous Oxide Isotope Analyzer for Measuring Bioremediation”. United States: N. p., 2019. Web. doi:10.2172/1527162.
Gupta, Manish. SBIR Phase I Final Report: “Site-Specific Nitrous Oxide Isotope Analyzer for Measuring Bioremediation”. United States. doi:10.2172/1527162.
Gupta, Manish. Thu . "SBIR Phase I Final Report: “Site-Specific Nitrous Oxide Isotope Analyzer for Measuring Bioremediation”". United States. doi:10.2172/1527162. https://www.osti.gov/servlets/purl/1527162.
@article{osti_1527162,
title = {SBIR Phase I Final Report: “Site-Specific Nitrous Oxide Isotope Analyzer for Measuring Bioremediation”},
author = {Gupta, Manish},
abstractNote = {In Phase I, LGR demonstrated technical feasibility by fabricating a prototype system for quantification of N2O, 15N14NO, 14N15NO, and 14N14N18O at ambient concentrations. The prototype was tested on a variety of gas cylinders and found to quantify [N2O], del15N, del15Nalpha, del15Nbeta, and del18O in N2O with a precision of ±0.05 ppb, ±0.4 ‰, ±0.45 ‰, ±0.6 ‰, and ±1.2 ‰ respectively (1sigma, 100s for [N2O] = 322 ppb). Measurements of calibrated and diluted gas standards demonstrated that the Phase I sensor was accurate to better than ±1ppb and ±1‰ for N2O concentration and isotope ratios, respectively, with a linear response over a wide dynamic range of [N2O] = 150 – 10000 ppb. Potential cross-interferences with ambient water vapor and carbon dioxide were identified and mitigated by using front-end sample conditioning techniques. The instrument was then deployed in conjunction with Professor Joseph von Fischer’s group in the Department of Biology at Colorado State University, where it was interfaced to a novel, collapsible Mylar chamber for continuous flow studies of soil gases with minimal pressure effects. This setup was used in conjunction with 15N-labelling experiments to identify nitrification and denitrification pathways in soil samples obtained from NSF LTER sites.},
doi = {10.2172/1527162},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {6}
}