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Title: Dynamical Self-Assembly: Constrained phase separation and mesoscale dynamics in lipid membranes (Final Report)

Abstract

This final technical report summarizes scientific and technical achievements derived through the the award, DE-FG02-04ER46173 [2013-2016, 2016-2017, and 2017-2018(NCE)], for the grant proposal titled, “Dynamical Self-Assembly: Constrained phase separation and mesoscale dynamics in lipid membranes.” The effort was organized into three major scientific aims: (1) characterize the mechanism of inter-layer coupling of laterally phase-separated domains in stacked lipid bilayers; (2) characterize curvature-dependent dynamic reorganization in membrane milieu; and (3) characterize real-time effects of mechanochemical perturbations on lateral phase separation membrane bilayers. The cumulative weight of the activities, including experimental research and collaborative modeling, disseminated through the publications listed above and presentations, has addressed two over-arching objectives: (a) to abstract a physical science based understanding of fundamental rules that determine dynamic self-assembly processes in lipid bilayer membranes - a versatile class of biologically inspired interfacial complex fluids and (b) to translate these physical principles into quantitative design rules for the development of new classes of membrane-based complex materials that exhibit complex, co-operative, and adaptive behavior.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [2];  [2];  [3];  [4];  [5];  [5];  [6];  [7];  [2];  [8]
  1. Univ. of California, Davis, CA (United States)
  2. Univ. of California, San Diego, CA (United States)
  3. Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  4. Northern Illinois Univ., DeKalb, IL (United States)
  5. Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
  6. Linkoping Univ. (Sweden); Sweden Tech. Univ. (Sweden); Nanyang Tech. Univ. (Singapore)
  7. Nanyang Tech. Univ. (Singapore)
  8. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Publication Date:
Research Org.:
Univ. of California, Davis, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Materials Sciences & Engineering Division
OSTI Identifier:
1525891
Report Number(s):
DOE-UCDAVIS-46173
DOE Contract Number:  
FG02-04ER46173
Resource Type:
Technical Report
Resource Relation:
Related Information: see below
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; bio-inspired materials; structure and dynamics in soft matter; organization under non-equilibrium conditions; phase separation; self-assembly; membrane-mimetic materials

Citation Formats

Parikh, Atul N, Sinha, Sunil K, Sanborn, Jeremy, Patel, Mira, Ngassam, Viviane, Gettel, Doug, Wilkop, Thomas, Su, Wan-Chih, Ma, Yicong, Ghosh, Sajal K., Dilena, David, Sasaki, Daryl, Lurio, Laurence, Jiang, Zhang, Mukhopadhyay, Mrinmay, Liedberg, Bo, Kraut, Rachel, Lal, Ratnesh, and Noy, Aleksandr. Dynamical Self-Assembly: Constrained phase separation and mesoscale dynamics in lipid membranes (Final Report). United States: N. p., 2019. Web. doi:10.2172/1525891.
Parikh, Atul N, Sinha, Sunil K, Sanborn, Jeremy, Patel, Mira, Ngassam, Viviane, Gettel, Doug, Wilkop, Thomas, Su, Wan-Chih, Ma, Yicong, Ghosh, Sajal K., Dilena, David, Sasaki, Daryl, Lurio, Laurence, Jiang, Zhang, Mukhopadhyay, Mrinmay, Liedberg, Bo, Kraut, Rachel, Lal, Ratnesh, & Noy, Aleksandr. Dynamical Self-Assembly: Constrained phase separation and mesoscale dynamics in lipid membranes (Final Report). United States. doi:10.2172/1525891.
Parikh, Atul N, Sinha, Sunil K, Sanborn, Jeremy, Patel, Mira, Ngassam, Viviane, Gettel, Doug, Wilkop, Thomas, Su, Wan-Chih, Ma, Yicong, Ghosh, Sajal K., Dilena, David, Sasaki, Daryl, Lurio, Laurence, Jiang, Zhang, Mukhopadhyay, Mrinmay, Liedberg, Bo, Kraut, Rachel, Lal, Ratnesh, and Noy, Aleksandr. Wed . "Dynamical Self-Assembly: Constrained phase separation and mesoscale dynamics in lipid membranes (Final Report)". United States. doi:10.2172/1525891. https://www.osti.gov/servlets/purl/1525891.
@article{osti_1525891,
title = {Dynamical Self-Assembly: Constrained phase separation and mesoscale dynamics in lipid membranes (Final Report)},
author = {Parikh, Atul N and Sinha, Sunil K and Sanborn, Jeremy and Patel, Mira and Ngassam, Viviane and Gettel, Doug and Wilkop, Thomas and Su, Wan-Chih and Ma, Yicong and Ghosh, Sajal K. and Dilena, David and Sasaki, Daryl and Lurio, Laurence and Jiang, Zhang and Mukhopadhyay, Mrinmay and Liedberg, Bo and Kraut, Rachel and Lal, Ratnesh and Noy, Aleksandr},
abstractNote = {This final technical report summarizes scientific and technical achievements derived through the the award, DE-FG02-04ER46173 [2013-2016, 2016-2017, and 2017-2018(NCE)], for the grant proposal titled, “Dynamical Self-Assembly: Constrained phase separation and mesoscale dynamics in lipid membranes.” The effort was organized into three major scientific aims: (1) characterize the mechanism of inter-layer coupling of laterally phase-separated domains in stacked lipid bilayers; (2) characterize curvature-dependent dynamic reorganization in membrane milieu; and (3) characterize real-time effects of mechanochemical perturbations on lateral phase separation membrane bilayers. The cumulative weight of the activities, including experimental research and collaborative modeling, disseminated through the publications listed above and presentations, has addressed two over-arching objectives: (a) to abstract a physical science based understanding of fundamental rules that determine dynamic self-assembly processes in lipid bilayer membranes - a versatile class of biologically inspired interfacial complex fluids and (b) to translate these physical principles into quantitative design rules for the development of new classes of membrane-based complex materials that exhibit complex, co-operative, and adaptive behavior.},
doi = {10.2172/1525891},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {6}
}

Works referenced in this record:

Cholesterol Partition and Condensing Effect in Phase-Separated Ternary Mixture Lipid Multilayers
journal, March 2016


Third-Party ATP Sensing in Polymersomes: A Label-Free Assay of Enzyme Reactions in Vesicular Compartments
journal, August 2013


Cholesterol-Enriched Domain Formation Induced by Viral-Encoded, Membrane-Active Amphipathic Peptide
journal, January 2016

  • Hanson, Joshua M.; Gettel, Douglas L.; Tabaei, Seyed R.
  • Biophysical Journal, Vol. 110, Issue 1
  • DOI: 10.1016/j.bpj.2015.11.032

Lipid Membrane Deformation Accompanied by Disk-to-Ring Shape Transition of Cholesterol-Rich Domains
journal, July 2015

  • Ryu, Yong-Sang; Yoo, Daehan; Wittenberg, Nathan J.
  • Journal of the American Chemical Society, Vol. 137, Issue 27
  • DOI: 10.1021/jacs.5b04559

Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes
journal, May 2016

  • Ryu, Yong-Sang; Wittenberg, Nathan J.; Suh, Jeng-Hun
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep26823

Lithographically Defined Macroscale Modulation of Lateral Fluidity and Phase Separation Realized via Patterned Nanoporous Silica-Supported Phospholipid Bilayers
journal, October 2013

  • Kendall, Eric L.; Ngassam, Viviane N.; Gilmore, Sean F.
  • Journal of the American Chemical Society, Vol. 135, Issue 42
  • DOI: 10.1021/ja408434r

Thermal Annealing Triggers Collapse of Biphasic Supported Lipid Bilayers into Multilayer Islands
journal, April 2014

  • Gilmore, Sean F.; Sasaki, Darryl Y.; Parikh, Atul N.
  • Langmuir, Vol. 30, Issue 17
  • DOI: 10.1021/la5005424

Mixing, Diffusion, and Percolation in Binary Supported Membranes Containing Mixtures of Lipids and Amphiphilic Block Copolymers
journal, July 2014

  • Gettel, Douglas L.; Sanborn, Jeremy; Patel, Mira A.
  • Journal of the American Chemical Society, Vol. 136, Issue 29
  • DOI: 10.1021/ja5037308

Accurate calibration and control of relative humidity close to 100% by X-raying a DOPC multilayer
journal, January 2015

  • Ma, Yicong; Ghosh, Sajal K.; Bera, Sambhunath
  • Physical Chemistry Chemical Physics, Vol. 17, Issue 5
  • DOI: 10.1039/C4CP04407J

Carbon Nanotube Porins in Amphiphilic Block Copolymers as Fully Synthetic Mimics of Biological Membranes
journal, October 2018

  • Sanborn, Jeremy R.; Chen, Xi; Yao, Yun-Chiao
  • Advanced Materials, Vol. 30, Issue 51
  • DOI: 10.1002/adma.201803355

Mixing Water, Transducing Energy, and Shaping Membranes: Autonomously Self-Regulating Giant Vesicles
journal, February 2016


Interaction of sphingomyelinase with sphingomyelin-containing supported membranes
journal, January 2013

  • Ngassam, Viviane N.; Oliver, Ann E.; Dang, Phuong N.
  • Soft Matter, Vol. 9, Issue 43
  • DOI: 10.1039/C3SM51855H

Role of Squalene in the Organization of Monolayers Derived from Lipid Extracts of Halobacterium salinarum
journal, June 2013

  • Gilmore, Sean F.; Yao, Andrew I.; Tietel, Zipora
  • Langmuir, Vol. 29, Issue 25
  • DOI: 10.1021/la401412t

Lipid Membrane Domains for the Selective Adsorption and Surface Patterning of Conjugated Polyelectrolytes
journal, April 2013

  • Sasaki, Darryl Y.; Zawada, Nicole; Gilmore, Sean F.
  • Langmuir, Vol. 29, Issue 17
  • DOI: 10.1021/la400454c

Biologically inspired far-from-equilibrium materials
journal, February 2019

  • Srinivasarao, Mohan; Iannacchione, Germano S.; Parikh, Atul N.
  • MRS Bulletin, Vol. 44, Issue 2
  • DOI: 10.1557/mrs.2019.24

On-Demand Self-Assembly of Supported Membranes Using Sacrificial, Anhydrobiotic Sugar Coats
journal, December 2013

  • Wilkop, Thomas E.; Sanborn, Jeremy; Oliver, Ann E.
  • Journal of the American Chemical Society, Vol. 136, Issue 1
  • DOI: 10.1021/ja410866w

Medium Matters: Order through Fluctuations?
journal, June 2015


Anomalous partitioning of water in coexisting liquid phases of lipid multilayers near 100% relative humidity
journal, January 2016

  • Ma, Yicong; Ghosh, Sajal K.; Bera, Sambhunath
  • Physical Chemistry Chemical Physics, Vol. 18, Issue 2
  • DOI: 10.1039/C5CP04703J