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Title: Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses

Journal Article · · IUCrJ
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  1. Uppsala Univ., Uppsala (Sweden)
  2. Uppsala Univ., Uppsala (Sweden); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  4. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  5. National Univ. of Singapore (Singapore)
  6. Uppsala Univ., Uppsala (Sweden); Bhabha Atomic Research Center, Mumbai (India)
  7. Arizona State Univ., Tempe, AZ (United States); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  8. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
  9. Uppsala Univ., Uppsala (Sweden); KTH Royal Institute of Technology, Stockholm (Sweden)
  10. Uppsala Univ., Uppsala (Sweden); Czech Academy of Science, Prague (Czech Republic)
  11. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  12. Brookhaven National Lab. (BNL), Upton, NY (United States)
  13. Uppsala Univ., Uppsala (Sweden); Institute of Physics AS CR, Prague (Czech Republic)
+ Show Author Affiliations

This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. AerosolizedOmono River virusparticles of ~40 nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from ~35 to ~300 nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 × 1012photons per µm2per pulse. The full-width of the focus at half-maximum was estimated to be 500 nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.

Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Brookhaven National Laboratory (BNL), Upton, NY (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-76SF00515; SC00112704; AC02-05CH11231; SC0012704
OSTI ID:
1368570
Alternate ID(s):
OSTI ID: 1376140; OSTI ID: 1413724; OSTI ID: 1525396
Report Number(s):
BNL-114080-2017-JA; BNL-114080-2017-JAAM; IUCRAJ; PII: S2052252517003591
Journal Information:
IUCrJ, Vol. 4, Issue 3; ISSN 2052-2525
Publisher:
International Union of CrystallographyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 37 works
Citation information provided by
Web of Science

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Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
  • Daurer, Benedikt
  • Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); University of Gothenburg, University of Jyvaskyla, University of Helsinki, CFEL, The University of Tokyo, Arizona State University, La Trobe University, University of Wisconsin, SLAC National Accelerator Laboratory, RIKEN SPring-8 Center, University of Chicago, Argonne National Laboratory, Northeastern Illinois University https://doi.org/10.11577/1349716
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Cited By (31)

The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters journal January 2020
Ultracompact 3D microfluidics for time-resolved structural biology journal January 2020
A statistical approach to detect protein complexes at X-ray free electron laser facilities journal December 2018
Ultrafast nonthermal heating of water initiated by an X-ray Free-Electron Laser journal May 2018
Nanometre-sized droplets from a gas dynamic virtual nozzle journal July 2019
Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging journal April 2019
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation journal April 2019
Characterizing the intrinsic properties of individual XFEL pulses via single-particle diffraction journal January 2020
Considerations for three-dimensional image reconstruction from experimental data in coherent diffractive imaging journal September 2018
Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams journal September 2018
Experimental 3D coherent diffractive imaging from photon-sparse random projections journal March 2019
Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses. Corrigendum journal April 2019
Mask-based approach to phasing of single-particle diffraction data. II. Likelihood-based selection criteria journal January 2019
Electrospray sample injection for single-particle imaging with x-ray lasers journal May 2019
Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
  • Daurer, Benedikt
  • Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); University of Gothenburg, University of Jyvaskyla, University of Helsinki, CFEL, The University of Tokyo, Arizona State University, La Trobe University, University of Wisconsin, SLAC National Accelerator Laboratory, RIKEN SPring-8 Center, University of Chicago, Argonne National Laboratory, Northeastern Illinois University https://doi.org/10.11577/1349716
dataset January 2016
Supervised classification methods for flash X-ray single particle diffraction imaging journal January 2019
Single Particle Study by X-Ray Diffraction: Crystallographic Approach journal November 2019
Software Platform for European XFEL: Towards Online Experimental Data Analysis text January 2018
Megahertz single-particle imaging at the European XFEL text January 2020
Megahertz single-particle imaging at the European XFEL journal May 2020
Software Platform for European XFEL: Towards Online Experimental Data Analysis journal November 2018
Single Particle Study by X-Ray Diffraction: Crystallographic Approach journal November 2019
Ultracompact 3D microfluidics for time-resolved structural biology text January 2020
Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams text January 2018
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation text January 2019
X-ray microscopy journal February 2017
Considerations for three-dimensional image reconstruction from experimental data in coherent diffractive imaging text January 2018
Experimental 3D coherent diffractive imaging from photon-sparse random projections text January 2019
X-ray microscopy journal July 1965
Ultrafast nonthermal heating of water initiated by an X-ray Free-Electron Laser text January 2018
Ultracompact 3D microfluidics for time-resolved structural biology text January 2020

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Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
X-ray diffraction
free-electron laser
flash X-ray imaging
diffraction before destruction
virus
Omono River virus
OmRV
59 BASIC BIOLOGICAL SCIENCES
omono river virus