Structural and functional insights into the unique CBS–CP12 fusion protein family in cyanobacteria

Hackenberg, Claudia; Hakanpää, Johanna; Cai, Fei; Antonyuk, Svetlana; Eigner, Caroline; Meissner, Sven; Laitaoja, Mikko; Jänis, Janne; Kerfeld, Cheryl A.; Dittmann, Elke; Lamzin, Victor S.

doi:10.1073/pnas.1806668115

Title: Structural and functional insights into the unique CBS–CP12 fusion protein family in cyanobacteria

Journal Article · Mon Jun 18 00:00:00 EDT 2018 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1806668115· OSTI ID:1515797

Hackenberg, Claudia; Hakanpää, Johanna; Cai, Fei; Antonyuk, Svetlana; Eigner, Caroline; Meissner, Sven; Laitaoja, Mikko; Jänis, Janne;

; Dittmann, Elke; Lamzin, Victor S.

Cyanobacteria are important photosynthetic organisms inhabiting a range of dynamic environments. This phylum is distinctive among photosynthetic organisms in containing genes encoding uncharacterized cystathionine β-synthase (CBS)–chloroplast protein (CP12) fusion proteins. These consist of two domains, each recognized as stand-alone photosynthetic regulators with different functions described in cyanobacteria (CP12) and plants (CP12 and CBSX). Here we show that CBS–CP12 fusion proteins are encoded in distinct gene neighborhoods, several unrelated to photosynthesis. Most frequently, CBS–CP12 genes are in a gene cluster with thioredoxin A (TrxA), which is prevalent in bloom-forming, marine symbiotic, and benthic mat cyanobacteria. Focusing on a CBS–CP12 fromMicrocystis aeruginosaPCC 7806 encoded in a gene cluster with TrxA, we reveal that the domain fusion led to the formation of a hexameric protein. We show that the CP12 domain is essential for hexamerization and contains an ordered, previously structurally uncharacterized N-terminal region. We provide evidence that CBS–CP12, while combining properties of both regulatory domains, behaves different from CP12 and plant CBSX. It does not form a ternary complex with phosphoribulokinase (PRK) and glyceraldehyde-3-phosphate dehydrogenase. Instead, CBS–CP12 decreases the activity of PRK in an AMP-dependent manner. We propose that the novel domain architecture and oligomeric state of CBS–CP12 expand its regulatory function beyond those of CP12 in cyanobacteria.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Michigan State Univ., East Lansing, MI (United States). DOE Plant Research Laboratory

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); German Research Foundation (DFG); European Union (EU)

Grant/Contract Number:: FG02-91ER20021; AC02-05CH11231; Di910/10-1; A70135; 731077

OSTI ID:: 1515797

Alternate ID(s):: OSTI ID: 1488747; OSTI ID: 1603658

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Vol. 115 Journal Issue: 27; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 15 works

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