Genetic Control of Flowering in Switchgrass
- US Dept. of Agriculture (USDA), Madison, WI (United States). Agricultural Research Service (ARS)
- Purdue Univ., West Lafayette, IN (United States)
Objective 1. Conduct GWAS of flowering time and identify genes for control and regulation of flowering time. Two switchgrass association panels were used to complete this objective, the northern and southern panels. Together, the two panels consisted of 985 switchgrass genotypes representing 110 accessions and the entire geographic range of switchgrass in the USA. Genotyping of both panels has been completed. Paul Grabowski completed the GWAS analysis of the northern panel, discovering numerous SNP markers within putative flowering time genes that are significantly associated with flowering time phenotype. The most important of these is an ortholog of the FT gene in rice, brachypodium, and arabidopsis. The manuscript describing these results has been published. The second manuscript, involving the second panel, is still under development. Objective 2. Validate genes associated with flowering time in the segregating populations. Three switchgrass double crosses (F2s) were successfully created in October 2013, each having a sufficient number of viable seeds to generate mapping populations. Each F2 mapping population is a cross between two siblings from an F1 cross of an early-flowering upland plant (Summer) and a late-flowering lowland plant (Ellsworth). A total of 286 genotypes were evaluated at two field locations (DeKalb, IL and West Lafayette, IN). Several loci were identified as critical for regulating flowering time, including validation of loci identified from previous studies, and several that are co-located with orthologs of candidate genes involved in the circadian clock, vernalization, and floral meristem development pathways.
- Research Organization:
- US Dept. of Agriculture (USDA), Madison, WI (United States). Agricultural Research Service (ARS)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- DOE Contract Number:
- SC0008180
- OSTI ID:
- 1513212
- Report Number(s):
- DE-SC0008180
- Country of Publication:
- United States
- Language:
- English
Genome-Wide Association Study in Pseudo-F2 Populations of Switchgrass Identifies Genetic Loci Affecting Heading and Anthesis Dates
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journal | September 2018 |
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Genome-Wide Association Study in Pseudo-F2 Populations of Switchgrass Identifies Genetic Loci Affecting Heading and Anthesis Dates
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