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Title: Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis.

Abstract

Most glycosylation reactions require activated glycosyl donors in the form of nucleotide sugars to drive processes such as posttranslational modifications and polysaccharide biosynthesis. Most plant cell wall polysaccharides are biosynthesized in the Golgi apparatus from cytosolic-derived nucleotide sugars, which are actively transferred into the Golgi lumen by nucleotide sugar transporters (NSTs). An exception is UDP-xylose, which is biosynthesized in both the cytosol and the Golgi lumen by a family of UDP-xylose synthases. The NST-based transport of UDP-xylose into the Golgi lumen would appear to be redundant. However, employing a recently developed approach, we identified three UDP-xylose transporters in the Arabidopsis thaliana NST family and designated them UDP-XYLOSE TRANSPORTER1 (UXT1) to UXT3. All three transporters localize to the Golgi apparatus, and UXT1 also localizes to the endoplasmic reticulum. Mutants in UXT1 exhibit ~30% reduction in xylose in stem cell walls. These findings support the importance of the cytosolic UDP-xylose pool and UDP-xylose transporters in cell wall biosynthesis.

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1512091
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Plant Cell
Additional Journal Information:
Journal Volume: 27; Journal Issue: 4; Journal ID: ISSN 1040-4651
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English

Citation Formats

Ebert, Berit, Rautengarten, Carsten, Guo, Xiaoyuan, Xiong, Guangyan, Stonebloom, Solomon, Smith-Moritz, Andreia M, Herter, Thomas, Chan, Leanne Jade G, Adams, Paul D, Petzold, Christopher J, Pauly, Markus, Willats, William GT, Heazlewood, Joshua L, and Scheller, Henrik Vibe. Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis.. United States: N. p., 2015. Web. doi:10.1105/tpc.114.133827.
Ebert, Berit, Rautengarten, Carsten, Guo, Xiaoyuan, Xiong, Guangyan, Stonebloom, Solomon, Smith-Moritz, Andreia M, Herter, Thomas, Chan, Leanne Jade G, Adams, Paul D, Petzold, Christopher J, Pauly, Markus, Willats, William GT, Heazlewood, Joshua L, & Scheller, Henrik Vibe. Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis.. United States. doi:10.1105/tpc.114.133827.
Ebert, Berit, Rautengarten, Carsten, Guo, Xiaoyuan, Xiong, Guangyan, Stonebloom, Solomon, Smith-Moritz, Andreia M, Herter, Thomas, Chan, Leanne Jade G, Adams, Paul D, Petzold, Christopher J, Pauly, Markus, Willats, William GT, Heazlewood, Joshua L, and Scheller, Henrik Vibe. Wed . "Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis.". United States. doi:10.1105/tpc.114.133827.
@article{osti_1512091,
title = {Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis.},
author = {Ebert, Berit and Rautengarten, Carsten and Guo, Xiaoyuan and Xiong, Guangyan and Stonebloom, Solomon and Smith-Moritz, Andreia M and Herter, Thomas and Chan, Leanne Jade G and Adams, Paul D and Petzold, Christopher J and Pauly, Markus and Willats, William GT and Heazlewood, Joshua L and Scheller, Henrik Vibe},
abstractNote = {Most glycosylation reactions require activated glycosyl donors in the form of nucleotide sugars to drive processes such as posttranslational modifications and polysaccharide biosynthesis. Most plant cell wall polysaccharides are biosynthesized in the Golgi apparatus from cytosolic-derived nucleotide sugars, which are actively transferred into the Golgi lumen by nucleotide sugar transporters (NSTs). An exception is UDP-xylose, which is biosynthesized in both the cytosol and the Golgi lumen by a family of UDP-xylose synthases. The NST-based transport of UDP-xylose into the Golgi lumen would appear to be redundant. However, employing a recently developed approach, we identified three UDP-xylose transporters in the Arabidopsis thaliana NST family and designated them UDP-XYLOSE TRANSPORTER1 (UXT1) to UXT3. All three transporters localize to the Golgi apparatus, and UXT1 also localizes to the endoplasmic reticulum. Mutants in UXT1 exhibit ~30% reduction in xylose in stem cell walls. These findings support the importance of the cytosolic UDP-xylose pool and UDP-xylose transporters in cell wall biosynthesis.},
doi = {10.1105/tpc.114.133827},
journal = {Plant Cell},
issn = {1040-4651},
number = 4,
volume = 27,
place = {United States},
year = {2015},
month = {4}
}