MBIR: A cryo-ET 3D reconstruction method that effectively minimizes missing wedge artifacts and restores missing information

Yan, Rui; Venkatakrishnan, Singanallur V.; Liu, Jun; Bouman, Charles A.; Jiang, Wen

doi:10.1016/j.jsb.2019.03.002

Title: MBIR: A cryo-ET 3D reconstruction method that effectively minimizes missing wedge artifacts and restores missing information

Journal Article · Mon Mar 11 00:00:00 EDT 2019 · Journal of Structural Biology

DOI:https://doi.org/10.1016/j.jsb.2019.03.002· OSTI ID:1511930

Yan, Rui ^[1]; Venkatakrishnan, Singanallur V. ^[2]; Liu, Jun ^[3]; Bouman, Charles A. ^[4];

^[1]

Purdue Univ., West Lafayette, IN (United States). Markey Center for Structural Biology. Dept. of Biological Sciences
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Yale Univ., West Haven, CT (United States). School of Medicine. Dept. of Microbial Pathogenesis
Purdue Univ., West Lafayette, IN (United States). School of Electrical and Computer Engineering

Cryo-Electron Tomography (cryo-ET) has become an essential technique in revealing cellular and macromolecular assembly structures in their native states. However, due to radiation damage and the limited tilt range, cryo-ET suffers from low contrast and missing wedge artifacts, which limits the tomograms to low resolution and hinders further biological interpretation. We applied the Model-Based Iterative Reconstruction (MBIR) method to obtain tomographic 3D reconstructions of experimental cryo-ET datasets and demonstrated the advantages of MBIR in contrast improvement, missing wedge artifacts reduction, missing information restoration, and subtomogram averaging compared with other reconstruction approaches. Considering the outstanding reconstruction quality, MBIR has a great potential in the determination of high resolution biological structures with cryo-ET.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Purdue Univ., West Lafayette, IN (United States)

Sponsoring Organization:: USDOE; National Inst. of Health (NIH) (United States)

Grant/Contract Number:: AC05-00OR22725; 1U01NS110437

OSTI ID:: 1511930

Journal Information:: Journal of Structural Biology, Vol. 206, Issue 2; ISSN 1047-8477

Publisher:: ElseiverCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 20 works

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