A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover
Abstract
In a biorefinery producing cellulosic biofuels, biomass pretreatment will significantly influence the efficacy of enzymatic hydrolysis and microbial fermentation. Comparison of different biomass pretreatment techniques by studying the impact of pretreatment on downstream operations at industrially relevant conditions and performing comprehensive mass balances will help focus attention on necessary process improvements, and thereby help reduce the cost of biofuel production. An on-going collaboration between the three US Department of Energy (DOE) funded bioenergy research centers (Great Lakes Bioenergy Research Center (GLBRC), Joint BioEnergy Institute (JBEI) and BioEnergy Science Center (BESC)) has given us a unique opportunity to compare the performance of three pretreatment processes, notably dilute acid (DA), ionic liquid (IL) and ammonia fiber expansion (AFEXTM), using the same source of corn stover. Separate hydrolysis and fermentation (SHF) was carried out using various combinations of commercially available enzymes and engineered yeast (Saccharomyces cerevisiae 424A) strain. The optimal commercial enzyme combination (Ctec2: Htec2: Multifect Pectinase, percentage total protein loading basis) was evaluated for each pretreatment with a microplate-based assay using milled pretreated solids at 0.2% glucan loading and 15 mg total protein loading/g of glucan. The best enzyme combinations were 67:33:0 for DA, 39:33:28 for IL and 67:17:17 for AFEX. Themore »
- Authors:
-
- Michigan State Univ., East Lansing, MI (United States). Dept. of Chemical Engineering and Materials Science. Dept. of Energy (DOE) Great Lakes Bioenergy Research Center (GLBRC)
- Michigan State Univ., East Lansing, MI (United States). Dept. of Chemical Engineering and Materials Science. Dept. of Energy (DOE) Great Lakes Bioenergy Research Center (GLBRC); Univ. of Wisconsin, Madison, WI (United States). Dept. of Biochemistry. Dept. of Energy (DOE) Great Lakes Bioenergy Research Center (GLBRC)
- Jilin TuoPai Agriculture Products Development Ltd, Jilin (China)
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States). Deconstruction Division; Sandia National Lab. (SNL-CA), Livermore, CA (United States). Biological and Material Science Center
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of California, Riverside, CA (United States). Dept. of Chemical and Environmental Engineering. Bourns College of Engineering. Center for Environmental Research and Technology (CE-CERT)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of California, Riverside, CA (United States). Center for Environmental Research and Technology (CE-CERT). Bourns College of Engineering
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1511397
- Grant/Contract Number:
- AC02-05CH11231; FC02-07ER64494
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 1754-6834
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; AFEX; dilute acid; ionic liquid; pretreatment; enzymatic hydrolysis; cellulosic ethanol
Citation Formats
Uppugundla, Nirmal, da Costa Sousa, Leonardo, Chundawat, Shishir P. S., Yu, Xiurong, Simmons, Blake, Singh, Seema, Gao, Xiadi, Kumar, Rajeev, Wyman, Charles E., Dale, Bruce E., and Balan, Venkatesh. A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover. United States: N. p., 2014.
Web. doi:10.1186/1754-6834-7-72.
Uppugundla, Nirmal, da Costa Sousa, Leonardo, Chundawat, Shishir P. S., Yu, Xiurong, Simmons, Blake, Singh, Seema, Gao, Xiadi, Kumar, Rajeev, Wyman, Charles E., Dale, Bruce E., & Balan, Venkatesh. A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover. United States. https://doi.org/10.1186/1754-6834-7-72
Uppugundla, Nirmal, da Costa Sousa, Leonardo, Chundawat, Shishir P. S., Yu, Xiurong, Simmons, Blake, Singh, Seema, Gao, Xiadi, Kumar, Rajeev, Wyman, Charles E., Dale, Bruce E., and Balan, Venkatesh. 2014.
"A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover". United States. https://doi.org/10.1186/1754-6834-7-72. https://www.osti.gov/servlets/purl/1511397.
@article{osti_1511397,
title = {A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover},
author = {Uppugundla, Nirmal and da Costa Sousa, Leonardo and Chundawat, Shishir P. S. and Yu, Xiurong and Simmons, Blake and Singh, Seema and Gao, Xiadi and Kumar, Rajeev and Wyman, Charles E. and Dale, Bruce E. and Balan, Venkatesh},
abstractNote = {In a biorefinery producing cellulosic biofuels, biomass pretreatment will significantly influence the efficacy of enzymatic hydrolysis and microbial fermentation. Comparison of different biomass pretreatment techniques by studying the impact of pretreatment on downstream operations at industrially relevant conditions and performing comprehensive mass balances will help focus attention on necessary process improvements, and thereby help reduce the cost of biofuel production. An on-going collaboration between the three US Department of Energy (DOE) funded bioenergy research centers (Great Lakes Bioenergy Research Center (GLBRC), Joint BioEnergy Institute (JBEI) and BioEnergy Science Center (BESC)) has given us a unique opportunity to compare the performance of three pretreatment processes, notably dilute acid (DA), ionic liquid (IL) and ammonia fiber expansion (AFEXTM), using the same source of corn stover. Separate hydrolysis and fermentation (SHF) was carried out using various combinations of commercially available enzymes and engineered yeast (Saccharomyces cerevisiae 424A) strain. The optimal commercial enzyme combination (Ctec2: Htec2: Multifect Pectinase, percentage total protein loading basis) was evaluated for each pretreatment with a microplate-based assay using milled pretreated solids at 0.2% glucan loading and 15 mg total protein loading/g of glucan. The best enzyme combinations were 67:33:0 for DA, 39:33:28 for IL and 67:17:17 for AFEX. The amounts of sugar (kg) (glucose: xylose: total gluco- and xylo-oligomers) per 100 kg of untreated corn stover produced after 72 hours of 6% glucan loading enzymatic hydrolysis were: DA (25:2:2), IL (31:15:2) and AFEX (26:13:7). Additionally, the amounts of ethanol (kg) produced per 100 kg of untreated corn stover and the respective ethanol metabolic yield (%) achieved with exogenous nutrient supplemented fermentations were: DA (14.0, 92.0%), IL (21.2, 93.0%) and AFEX (20.5, 95.0%), respectively. The reason for lower ethanol yield for DA is because most of the xylose produced during the pretreatment was removed and not converted to ethanol during fermentation. Compositional analysis of the pretreated biomass solids showed no significant change in composition for AFEX treated corn stover, while about 85% of hemicellulose was solubilized after DA pretreatment, and about 90% of lignin was removed after IL pretreatment. As expected, the optimal commercial enzyme combination was different for the solids prepared by different pretreatment technologies. Due to loss of nutrients during the pretreatment and washing steps, DA and IL pretreated hydrolysates required exogenous nutrient supplementation to ferment glucose and xylose efficiently, while AFEX pretreated hydrolysate did not require nutrient supplementation.},
doi = {10.1186/1754-6834-7-72},
url = {https://www.osti.gov/biblio/1511397},
journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = ,
volume = 7,
place = {United States},
year = {Tue May 13 00:00:00 EDT 2014},
month = {Tue May 13 00:00:00 EDT 2014}
}
Web of Science
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journal, December 2015
- Baral, Nawa Raj; Shah, Ajay
- Biofuels, Bioproducts and Biorefining, Vol. 10, Issue 1
Ethanol production potential from AFEX™ and steam-exploded sugarcane residues for sugarcane biorefineries
journal, May 2018
- Mokomele, Thapelo; da Costa Sousa, Leonardo; Balan, Venkatesh
- Biotechnology for Biofuels, Vol. 11, Issue 1
Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review
journal, January 2017
- Kumar, Adepu Kiran; Sharma, Shaishav
- Bioresources and Bioprocessing, Vol. 4, Issue 1
Fermentation strategy for second generation ethanol production from sugarcane bagasse hydrolyzate by Spathaspora passalidarum and Scheffersomyces stipitis: Fermentation Strategy for Second Generation Ethanol Production
journal, July 2017
- Nakanishi, Simone C.; Soares, Lauren B.; Biazi, Luiz Eduardo
- Biotechnology and Bioengineering, Vol. 114, Issue 10
An overview of key pretreatment processes for biological conversion of lignocellulosic biomass to bioethanol
journal, February 2015
- Maurya, Devendra Prasad; Singla, Ankit; Negi, Sangeeta
- 3 Biotech, Vol. 5, Issue 5
Impact of lignin polymer backbone esters on ionic liquid pretreatment of poplar
text, January 2017
- Kim, Kwang H.; Dutta, Tanmoy; Ralph, John
- BioMed Central
Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review
journal, January 2017
- Kumar, Adepu Kiran; Sharma, Shaishav
- Bioresources and Bioprocessing, Vol. 4, Issue 1
Ethanol production potential from AFEX™ and steam-exploded sugarcane residues for sugarcane biorefineries
journal, May 2018
- Mokomele, Thapelo; da Costa Sousa, Leonardo; Balan, Venkatesh
- Biotechnology for Biofuels, Vol. 11, Issue 1
Lipid metabolism of phenol-tolerant Rhodococcus opacus strains for lignin bioconversion
journal, December 2018
- Henson, William R.; Hsu, Fong-Fu; Dantas, Gautam
- Biotechnology for Biofuels, Vol. 11, Issue 1
Dry biorefining maximizes the potentials of simultaneous saccharification and co-fermentation for cellulosic ethanol production
journal, October 2017
- Liu, Gang; Zhang, Qiang; Li, Hongxing
- Biotechnology and Bioengineering, Vol. 115, Issue 1
Using Populus as a lignocellulosic feedstock for bioethanol
journal, February 2015
- Porth, Ilga; El-Kassaby, Yousry A.
- Biotechnology Journal, Vol. 10, Issue 4
Co-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol
journal, June 2017
- Lee, Cho-Ryong; Sung, Bong Hyun; Lim, Kwang-Mook
- Scientific Reports, Vol. 7, Issue 1
Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties
journal, September 2019
- Bhalla, Aditya; Cai, Charles M.; Xu, Feng
- Biotechnology for Biofuels, Vol. 12, Issue 1
Typical conversion of lignocellulosic biomass into reducing sugars using dilute acid hydrolysis and alkaline pretreatment
journal, April 2016
- Loow, Yu-Loong; Wu, Ta Yeong; Md. Jahim, Jamaliah
- Cellulose, Vol. 23, Issue 3
Facile isothermal solid acid catalyzed ionic liquid pretreatments to enhance the combined sugars production from Arundo donax Linn.
journal, August 2016
- You, Tingting; Shao, Lupeng; Wang, Ruizhen
- Biotechnology for Biofuels, Vol. 9, Issue 1
Sugar loss and enzyme inhibition due to oligosaccharide accumulation during high solids-loading enzymatic hydrolysis
journal, November 2015
- Xue, Saisi; Uppugundla, Nirmal; Bowman, Michael J.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Impact of lignin polymer backbone esters on ionic liquid pretreatment of poplar
journal, April 2017
- Kim, Kwang Ho; Dutta, Tanmoy; Ralph, John
- Biotechnology for Biofuels, Vol. 10, Issue 1
Overcoming challenges in lignocellulosic biomass pretreatment for second-generation (2G) sugar production: emerging role of nano, biotechnological and promising approaches
journal, May 2019
- Antunes, Felipe Antonio Fernandes; Chandel, Anuj Kumar; Terán-Hilares, Ruly
- 3 Biotech, Vol. 9, Issue 6
Co-fermentation using Recombinant Saccharomyces cerevisiae Yeast Strains Hyper-secreting Different Cellulases for the Production of Cellulosic Bioethanol
journal, June 2017
- Lee, Cho-Ryong; Sung, Bong Hyun; Lim, Kwang-Mook
- Scientific Reports, Vol. 7, Issue 1
Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production
journal, May 2017
- Jonathan, M. C.; DeMartini, J.; Van Stigt Thans, S.
- Biotechnology for Biofuels, Vol. 10, Issue 1
The effect of alkali-soluble lignin on purified core cellulase and hemicellulase activities during hydrolysis of extractive ammonia-pretreated lignocellulosic biomass
journal, June 2018
- Zhou, Linchao; da Costa Sousa, Leonardo; Dale, Bruce E.
- Royal Society Open Science, Vol. 5, Issue 6
Industrially relevant hydrolyzability and fermentability of sugarcane bagasse improved effectively by glycerol organosolv pretreatment
journal, March 2016
- Sun, Fubao Fuelbiol; Zhao, Xiaoqin; Hong, Jiapeng
- Biotechnology for Biofuels, Vol. 9, Issue 1
Biotechnology Application of Pretreated Biomass
book, October 2019
- Treichel, Helen; Fongaro, Gislaine; Scapini, Thamarys
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Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties
journal, September 2019
- Bhalla, Aditya; Cai, Charles M.; Xu, Feng
- Biotechnology for Biofuels, Vol. 12, Issue 1
Sugar loss and enzyme inhibition due to oligosaccharide accumulation during high solids-loading enzymatic hydrolysis
journal, November 2015
- Xue, Saisi; Uppugundla, Nirmal; Bowman, Michael J.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Scaling up and benchmarking of ethanol production from pelletized pilot scale AFEX treated corn stover using Zymomonas mobilis 8b
journal, January 2016
- Sarks, Cory; Bals, Bryan D.; Wynn, Jim
- Biofuels, Vol. 7, Issue 3
Multi-Scale Variability Analysis of Wheat Straw-Based Ethanol Biorefineries Identifies Bioprocess Designs Robust Against Process Input Variations
journal, April 2020
- Nickel, David Benjamin; Fornell, Rickard; Janssen, Matty
- Frontiers in Energy Research, Vol. 8
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