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Title: Genome-Wide Association Study in Pseudo-F2 Populations of Switchgrass Identifies Genetic Loci Affecting Heading and Anthesis Dates

Journal Article · · Frontiers in Plant Science
 [1];  [2];  [3];  [4];  [5];  [1];  [1];  [6];  [2];  [6];  [6];  [7];  [1]
  1. Purdue Univ., West Lafayette, IN (United States)
  2. Univ. of Wisconsin System, Madison, WI (United States)
  3. Purdue Univ., West Lafayette, IN (United States); Sichuan Univ., Chengdu (China)
  4. United States Dept.of Agriculture-Agricultural Research Service, Madison, WI (United States)
  5. Purdue Univ., West Lafayette, IN (United States); Carnegie Mellon Univ., Pittsburgh, PA (United States)
  6. Michigan State Univ., East Lansing, MI (United States)
  7. Univ. of Wisconsin System, Madison, WI (United States); United States Dept.of Agriculture-Agricultural Research Service, Madison, WI (United States)
+ Show Author Affiliations

Switchgrass (Panicum virgatum) is a native prairie grass and valuable bio-energy crop. The physiological change from juvenile to reproductive adult can draw important resources away from growth into producing reproductive structures, thereby limiting the growth potential of early flowering plants. Delaying the flowering of switchgrass is one approach by which to increase total biomass. The objective of this research was to identify genetic variants and candidate genes for controlling heading and anthesis in segregating switchgrass populations. Four pseudo-F2 populations (two pairs of reciprocal crosses) were developed from lowland (late flowering) and upland (early flowering) ecotypes, and heading and anthesis dates of these populations were collected in Lafayette, IN and DeKalb, IL in 2015 and 2016. Across 2 years, there was a 34- and 73-day difference in heading and a 52- and 75-day difference in anthesis at the Lafayette and DeKalb locations, respectively. A total of 37,901 single nucleotide polymorphisms obtained by exome capture sequencing of the populations were used in a genome-wide association study (GWAS) that identified five significant signals at three loci for heading and two loci for anthesis. Among them, a homolog of FLOWERING LOCUS T on chromosome 5b associated with heading date was identified at the Lafayette location across 2 years. A homolog of ARABIDOPSIS PSEUDO-RESPONSE REGULATOR 5, a light modulator in the circadian clock associated with heading date was detected on chromosome 8a across locations and years. These results demonstrate that genetic variants related to floral development could lend themselves to a long-term goal of developing late flowering varieties of switchgrass with high biomass yield.

Research Organization:
Univ. of Wisconsin System, Madison, WI (United States); Purdue Univ., West Lafayette, IN (United States); Agricultural Research Service, Peoria, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
FC02-07ER64494; SC0010631; SC0008180
OSTI ID:
1510729
Journal Information:
Frontiers in Plant Science, Vol. 9; ISSN 1664-462X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 10 works
Citation information provided by
Web of Science

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GWAS
candidate gene
heading
flowering time
Panicum virgatum