Isolation, Development, and Genomic Analysis of Bacillus megaterium SR7 for Growth and Metabolite Production Under Supercritical Carbon Dioxide

Freedman, Adam J. E.; Peet, Kyle C.; Boock, Jason T.; Penn, Kevin; Prather, Kristala L. J.; Thompson, Janelle R.

doi:10.3389/fmicb.2018.02152

Title: Isolation, Development, and Genomic Analysis of Bacillus megaterium SR7 for Growth and Metabolite Production Under Supercritical Carbon Dioxide

Journal Article · Tue Sep 25 00:00:00 EDT 2018 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2018.02152· OSTI ID:1473726

Freedman, Adam J. E.; Peet, Kyle C.; Boock, Jason T.; Penn, Kevin; Prather, Kristala L. J.; Thompson, Janelle R.

Supercritical carbon dioxide (scCO₂) is an attractive substitute for conventional organic solvents due to its unique transport and thermodynamic properties, its renewability and labile nature, and its high solubility for compounds such as alcohols, ketones, and aldehydes. However, biological systems that use scCO₂ are mainly limited to in vitro processes due to its strong inhibition of cell viability and growth. To solve this problem, we used a bioprospecting approach to isolate a microbial strain with the natural ability to grow while exposed to scCO₂. Enrichment culture and serial passaging of deep subsurface fluids from the McElmo Dome scCO₂ reservoir in aqueous media under scCO₂ headspace enabled the isolation of spore-forming strain Bacillus megaterium SR7. Sequencing and analysis of the complete 5.51 Mbp genome and physiological characterization revealed the capacity for facultative anaerobic metabolism, including fermentative growth on a diverse range of organic substrates. Supplementation of growth medium with L-alanine for chemical induction of spore germination significantly improved growth frequencies and biomass accumulation under scCO₂ headspace. Detection of endogenous fermentative compounds in cultures grown under scCO₂ represents the first observation of bioproduct generation and accumulation under this condition. Culturing development and metabolic characterization of B. megaterium SR7 represent initial advancements in the effort toward enabling exploitation of scCO₂ as a sustainable solvent for in vivo bioprocessing.

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Research Organization:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)

Grant/Contract Number:: SC0012555; P30-ES002109

OSTI ID:: 1473726

Alternate ID(s):: OSTI ID: 1510452

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 9; ISSN 1664-302X

Publisher:: Frontiers Media SACopyright Statement

Country of Publication:: Switzerland

Language:: English

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Cited by: 2 works

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59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
supercritical carbon dioxide
bioprospecting
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Bacillus megaterium
bioprocessing
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Title: Isolation, Development, and Genomic Analysis of Bacillus megaterium SR7 for Growth and Metabolite Production Under Supercritical Carbon Dioxide

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