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Title: Fungal endophytes of Populus trichocarpa alter host phenotype, gene expression and rhizobiome composition

Abstract

Mortierella and Ilyonectria genera include common species of soil fungi that are frequently detected as root endophytes in many plants, including Populus spp. However, the ecological roles of these and other endophytic fungi with respect to plant growth and function are still not well understood. The functional ecology of two key taxa from the P. trichocarpa rhizobiome, M. elongata PMI93 and I. europaea PMI82, was studied by coupling forest soil bioassays with environmental metatranscriptomics. Using soil bioassay experiments amended with fungal inoculants, M. elongata was observed to promote the growth of P. trichocarpa. This response was cultivar independent. In contrast, I. europaea had no visible effect on P. trichocarpa growth. Metatranscriptomic studies revealed that these fungi impacted rhizophytic and endophytic activities in P. trichocarpa and induced shifts in soil and root microbial communities. Differential expression of core genes in P. trichocarpa roots was observed in response to both fungal species. Expression of P. trichocarpa genes for lipid signaling and nutrient uptake were upregulated, and expression of genes associated with gibberellin signaling were altered in plants inoculated with M. elongata, but not I. europaea. Upregulation of genes for growth promotion, downregulation of genes for several leucine-rich repeat receptor kinases, and alterationmore » of expression of genes associated with plant defense responses (e.g., jasmonic acid, salicylic acid, and ethylene signal pathways) also suggest that M. elongata manipulates plant defenses while promoting plant growth.« less

Authors:
ORCiD logo [1];  [2]; ORCiD logo [3];  [4];  [5]; ORCiD logo [6]; ORCiD logo [6]; ORCiD logo [6];  [7];  [8];  [4]
  1. Univ. of Florida, Quincy, FL (United States)
  2. Michigan State Univ., East Lansing, MI (United States)
  3. Duke Univ., Durham, NC (United States); Univ. of Arkansas, Fayetteville, AR (United States)
  4. Duke Univ., Durham, NC (United States)
  5. Davis (United States)
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  7. Institut National de la Recherche Agronomique, Champenoux (France)
  8. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)
OSTI Identifier:
1509573
Alternate Identifier(s):
OSTI ID: 1619123
Grant/Contract Number:  
AC05-00OR22725; AC02-05CH11231; DEB1441715; DEB 1737898; ANR-11-LABX 0002 01
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Molecular Plant-Microbe Interactions
Additional Journal Information:
Journal Volume: 32; Journal Issue: 7; Journal ID: ISSN 0894-0282
Publisher:
APS Press - International Society for Molecular Plant-Microbe Interactions
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Liao, Hui -Ling, Bonito, Gregory, Rojas, J. Alejandro, Hameed, Khalid, Wu, Steven, Schadt, Christopher Warren, Labbe, Jessy L., Tuskan, Gerald A., Martin, Francis M., Grigoriev, Igor V., and Vilgalys, Rytas. Fungal endophytes of Populus trichocarpa alter host phenotype, gene expression and rhizobiome composition. United States: N. p., 2019. Web. doi:10.1094/MPMI-05-18-0133-R.
Liao, Hui -Ling, Bonito, Gregory, Rojas, J. Alejandro, Hameed, Khalid, Wu, Steven, Schadt, Christopher Warren, Labbe, Jessy L., Tuskan, Gerald A., Martin, Francis M., Grigoriev, Igor V., & Vilgalys, Rytas. Fungal endophytes of Populus trichocarpa alter host phenotype, gene expression and rhizobiome composition. United States. doi:10.1094/MPMI-05-18-0133-R.
Liao, Hui -Ling, Bonito, Gregory, Rojas, J. Alejandro, Hameed, Khalid, Wu, Steven, Schadt, Christopher Warren, Labbe, Jessy L., Tuskan, Gerald A., Martin, Francis M., Grigoriev, Igor V., and Vilgalys, Rytas. Wed . "Fungal endophytes of Populus trichocarpa alter host phenotype, gene expression and rhizobiome composition". United States. doi:10.1094/MPMI-05-18-0133-R. https://www.osti.gov/servlets/purl/1509573.
@article{osti_1509573,
title = {Fungal endophytes of Populus trichocarpa alter host phenotype, gene expression and rhizobiome composition},
author = {Liao, Hui -Ling and Bonito, Gregory and Rojas, J. Alejandro and Hameed, Khalid and Wu, Steven and Schadt, Christopher Warren and Labbe, Jessy L. and Tuskan, Gerald A. and Martin, Francis M. and Grigoriev, Igor V. and Vilgalys, Rytas},
abstractNote = {Mortierella and Ilyonectria genera include common species of soil fungi that are frequently detected as root endophytes in many plants, including Populus spp. However, the ecological roles of these and other endophytic fungi with respect to plant growth and function are still not well understood. The functional ecology of two key taxa from the P. trichocarpa rhizobiome, M. elongata PMI93 and I. europaea PMI82, was studied by coupling forest soil bioassays with environmental metatranscriptomics. Using soil bioassay experiments amended with fungal inoculants, M. elongata was observed to promote the growth of P. trichocarpa. This response was cultivar independent. In contrast, I. europaea had no visible effect on P. trichocarpa growth. Metatranscriptomic studies revealed that these fungi impacted rhizophytic and endophytic activities in P. trichocarpa and induced shifts in soil and root microbial communities. Differential expression of core genes in P. trichocarpa roots was observed in response to both fungal species. Expression of P. trichocarpa genes for lipid signaling and nutrient uptake were upregulated, and expression of genes associated with gibberellin signaling were altered in plants inoculated with M. elongata, but not I. europaea. Upregulation of genes for growth promotion, downregulation of genes for several leucine-rich repeat receptor kinases, and alteration of expression of genes associated with plant defense responses (e.g., jasmonic acid, salicylic acid, and ethylene signal pathways) also suggest that M. elongata manipulates plant defenses while promoting plant growth.},
doi = {10.1094/MPMI-05-18-0133-R},
journal = {Molecular Plant-Microbe Interactions},
issn = {0894-0282},
number = 7,
volume = 32,
place = {United States},
year = {2019},
month = {1}
}

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