Real‐time whole‐plant dynamics of heavy metal transport in Arabidopsis halleri and Arabidopsis thaliana by gamma‐ray imaging

Kajala, Kaisa; Walker, Katherine L.; Mitchell, Gregory S.; Krämer, Ute; Cherry, Simon R.; Brady, Siobhan M.

doi:10.1002/pld3.131

Title: Real‐time whole‐plant dynamics of heavy metal transport in Arabidopsis halleri and Arabidopsis thaliana by gamma‐ray imaging

Journal Article · Tue Apr 23 00:00:00 EDT 2019 · Plant Direct

DOI:https://doi.org/10.1002/pld3.131· OSTI ID:1508418

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Department of Plant Biology and Genome Center University of California Davis Davis California, Plant Ecophysiology Institute of Environmental Biology Utrecht University Utrecht The Netherlands
Department of Biomedical Engineering University of California Davis Davis California
Molecular Genetics and Physiology of Plants Ruhr University Bochum Bochum Germany
Department of Plant Biology and Genome Center University of California Davis Davis California

Abstract Heavy metals such as zinc are essential for plant growth, but toxic at high concentrations. Despite our knowledge of the molecular mechanisms of heavy metal uptake by plants, experimentally addressing the real‐time whole‐plant dynamics of heavy metal uptake and partitioning has remained a challenge. To overcome this, we applied a high sensitivity gamma‐ray imaging system to image uptake and transport of radioactive ⁶⁵ Zn in whole‐plant assays of Arabidopsis thaliana and the Zn hyperaccumulator Arabidopsis halleri . We show that our system can be used to quantitatively image and measure uptake and root‐to‐shoot translocation dynamics of zinc in real time. In the metal hyperaccumulator Arabidopsis halleri, ⁶⁵ Zn uptake and transport from its growth media to the shoot occurs rapidly and on time scales similar to those reported in rice. In transgenic A. halleri plants in which expression of the zinc transporter gene HMA 4 is suppressed by RNA i, ⁶⁵ Zn uptake is completely abolished.

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Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FG02-00ER41132

OSTI ID:: 1508418

Alternate ID(s):: OSTI ID: 1508420; OSTI ID: 1623579

Journal Information:: Plant Direct, Journal Name: Plant Direct Vol. 3 Journal Issue: 4; ISSN 2475-4455

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 6 works

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59 BASIC BIOLOGICAL SCIENCES
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Title: Real‐time whole‐plant dynamics of heavy metal transport in Arabidopsis halleri and Arabidopsis thaliana by gamma‐ray imaging

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