skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Metabolomics of sorghum roots during nitrogen stress reveals compromised metabolic capacity for salicylic acid biosynthesis

Journal Article · · Plant Direct
DOI:https://doi.org/10.1002/pld3.122· OSTI ID:1499822
 [1];  [2];  [3];  [3];  [4];  [4];  [4];  [4];  [2];  [3];  [5];  [6];  [6];  [1]
  1. Department of Horticulture and Landscape Architecture Colorado State University Fort Collins Colorado
  2. Joint Genome Institute Department of Energy Walnut Creek California
  3. Bioinformatics &, Computational Biology Iowa State University Ames Iowa
  4. Donald Danforth Plant Science Center St. Louis Missouri
  5. Plant and Environmental Genetics and Biochemistry Departments Clemson University Clemson South Carolina
  6. Center for Biotechnology University of Nebraska‐Lincoln Lincoln Nebraska
+ Show Author Affiliations

Abstract Sorghum ( Sorghum bicolor [L.] Moench) is the fifth most productive cereal crop worldwide with some hybrids having high biomass yield traits making it promising for sustainable, economical biofuel production. To maximize biofuel feedstock yields, a more complete understanding of metabolic responses to low nitrogen (N) will be useful for incorporation in crop improvement efforts. In this study, 10 diverse sorghum entries (including inbreds and hybrids) were field‐grown under low and full N conditions and roots were sampled at two time points for metabolomics and 16S amplicon sequencing. Roots of plants grown under low N showed altered metabolic profiles at both sampling dates including metabolites important in N storage and synthesis of aromatic amino acids. Complementary investigation of the rhizosphere microbiome revealed dominance by a single operational taxonomic unit ( OTU ) in an early sampling that was taxonomically assigned to the genus Pseudomonas . Abundance of this Pseudomonas OTU was significantly greater under low N in July and was decreased dramatically in September. Correlation of Pseudomonas abundance with root metabolites revealed a strong negative association with the defense hormone salicylic acid ( SA ) under full N but not under low N, suggesting reduced defense response. Roots from plants with N stress also contained reduced phenylalanine, a precursor for SA , providing further evidence for compromised metabolic capacity for defense response under low N conditions. Our findings suggest that interactions between biotic and abiotic stresses may affect metabolic capacity for plant defense and need to be concurrently prioritized as breeding programs become established for biofuels production on marginal soils.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23), Biological Systems Science Division (SC-23.2 )
Grant/Contract Number:
DE‐SC0014395; SC0014395; AC02-05CH11231
OSTI ID:
1499822
Alternate ID(s):
OSTI ID: 1503920; OSTI ID: 1508062
Journal Information:
Plant Direct, Journal Name: Plant Direct Vol. 3 Journal Issue: 3; ISSN 2475-4455
Publisher:
Wiley Blackwell (John Wiley & Sons)Copyright Statement
Country of Publication:
United Kingdom
Language:
English
Citation Metrics:
Cited by: 21 works
Citation information provided by
Web of Science

References (67)

Metabolic Control of Anaerobic Glycolysis (Overexpression of Lactate Dehydrogenase in Transgenic Tomato Roots Supports the Davies-Roberts Hypothesis and Points to a Critical Role for Lactate Secretion journal November 1994
Understanding and engineering beneficial plant-microbe interactions: plant growth promotion in energy crops journal November 2014
The Sorghum bicolor genome and the diversification of grasses journal January 2009
MetaboAnalyst 3.0—making metabolomics more meaningful journal April 2015
Alterations in Leaf Carbohydrate Metabolism in Response to Nitrogen Stress journal November 1988
Analysis of amino acid metabolism in the ear of maize mutants deficient in two cytosolic glutamine synthetase isoenzymes highlights the importance of asparagine for nitrogen translocation within sink organs: Analysis of nitrogen metabolism in maize ears journal November 2010
Diversity of nifH gene pools in the rhizosphere of two cultivars of sorghum ( Sorghum bicolor ) treated with contrasting levels of nitrogen fertilizer journal February 2008
Building the crops of tomorrow: advantages of symbiont-based approaches to improving abiotic stress tolerance journal June 2014
A Sorghum bicolor expression atlas reveals dynamic genotype-specific expression profiles for vegetative tissues of grain, sweet and bioenergy sorghums journal January 2014
Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys journal December 2015
XCMS:  Processing Mass Spectrometry Data for Metabolite Profiling Using Nonlinear Peak Alignment, Matching, and Identification journal February 2006
Root exudation of sugars, amino acids, and organic acids by maize as affected by nitrogen, phosphorus, potassium, and iron deficiency journal December 2010
Skyline: an open source document editor for creating and analyzing targeted proteomics experiments journal February 2010
A metabolomic study in oats ( A vena sativa ) highlights a drought tolerance mechanism based upon salicylate signalling pathways and the modulation of carbon, antioxidant and photo-oxidative metabolism : Metabolomics of oats under drought journal March 2015
Metabolomic profiling from leaves and roots of tomato ( Solanum lycopersicum L.) plants grown under nitrogen, phosphorus or potassium-deficient condition journal December 2015
Practical innovations for high-throughput amplicon sequencing journal September 2013
Metabolite fingerprinting of pennycress ( Thlaspi arvense L.) embryos to assess active pathways during oil synthesis journal February 2015
A new method for non-parametric multivariate analysis of variance: NON-PARAMETRIC MANOVA FOR ECOLOGY journal February 2001
Manifestations of Hybrid Vigor in Sorghum1 journal January 1963
Nitrogen‐induced changes in colony density and spore production of Erysiphe graminis f.sp. hordei on seedlings of six spring barley cultivars journal February 1997
Metabolomics in plant environmental physiology journal August 2013
Biosynthesis of salicylic acid in plants journal June 2009
Metabolite Profiling of Low-P Tolerant and Low-P Sensitive Maize Genotypes under Phosphorus Starvation and Restoration Conditions journal June 2015
2016 Billion-Ton Report: Advancing Domestic Resources for a Thriving Bioeconomy report July 2016
Primer and platform effects on 16S rRNA tag sequencing journal August 2015
Fitting Linear Mixed-Effects Models Using lme4 journal January 2015
RAMClust: A Novel Feature Clustering Method Enables Spectral-Matching-Based Annotation for Metabolomics Data journal June 2014
Search and clustering orders of magnitude faster than BLAST journal August 2010
Up-Regulation and Localization of Asparagine Synthetase in Tomato Leaves Infected by the Bacterial Pathogen Pseudomonas syringae journal June 2004
Successional Trajectories of Rhizosphere Bacterial Communities over Consecutive Seasons journal August 2015
High-parent heterosis for biomass yield in photoperiod-sensitive sorghum hybrids journal October 2014
The plant nitrogen mobilization promoted by Colletotrichum lindemuthianum in Phaseolus leaves depends on fungus pathogenicity journal September 2007
CLNR1, the AREA/NIT2-like global nitrogen regulator of the plant fungal pathogen Colletotrichum lindemuthianum is required for the infection cycle: A nitrogen regulator required for fungal pathogenicity journal April 2003
On a Test of Whether one of Two Random Variables is Stochastically Larger than the Other journal March 1947
PERANAN RIZOBAKTERI DAN FUNGI MIKORIZA ARBUSKULAR DALAM MENINGKATKAN EFISIENSI PENYERAPAN HARA SORGUM MANIS (Sorghum bicolor L. Moench) journal October 2014
Mapping QTLs and association of differentially expressed gene transcripts for multiple agronomic traits under different nitrogen levels in sorghum journal January 2016
Nitrogen uptake, assimilation and remobilization in plants: challenges for sustainable and productive agriculture journal March 2010
The two senescence-related markers, GS1 (cytosolic glutamine synthetase) and GDH (glutamate dehydrogenase), involved in nitrogen mobilization, are differentially regulated during pathogen attack and by stress hormones and reactive oxygen species in Nicotiana tabacum L. leaves journal December 2005
Population genomic and genome-wide association studies of agroclimatic traits in sorghum journal December 2012
Metabolomics – the link between genotypes and phenotypes journal January 2002
Trehalose and plant stress responses: friend or foe? journal July 2010
Asparagine Metabolic Pathways in Arabidopsis journal December 2015
Non-targeted Metabolomics in Diverse Sorghum Breeding Lines Indicates Primary and Secondary Metabolite Profiles Are Associated with Plant Biomass Accumulation and Photosynthesis journal July 2016
The untranslated leader sequence of the barley lipoxygenase 1 ( Lox1 ) gene confers embryo‐specific expression journal August 1998
Identification of differentially expressed genes between sorghum genotypes with contrasting nitrogen stress tolerance by genome-wide transcriptional profiling journal January 2014
Analysis of plant microbe interactions in the era of next generation sequencing technologies journal May 2014
Designing sorghum as a dedicated bioenergy feedstock journal October 2007
The aligned rank transform for nonparametric factorial analyses using only anova procedures conference January 2011
Analysis of composition of microbiomes: a novel method for studying microbial composition journal May 2015
Fitting Multivariate Models to Community Data: a Comment on Distance-Based Redundancy Analysis journal January 2001
Handbook of Parametric and Nonparametric Statistical Procedures book August 2003
Manipulating the soil microbiome to increase soil health and plant fertility journal May 2012
MicrobiomeAnalyst: a web-based tool for comprehensive statistical, visual and meta-analysis of microbiome data journal April 2017
Induction of Trehalase in Arabidopsis Plants Infected With the Trehalose-Producing Pathogen Plasmodiophora brassicae journal July 2002
Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection journal October 2014
Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens journal December 1998
Nitrogen signalling in plant interactions with associative and endophytic diazotrophic bacteria journal August 2014
Enabling Efficient and Confident Annotation of LC−MS Metabolomics Data through MS1 Spectrum and Time Prediction journal September 2016
‘Omics’ analyses of regulatory networks in plant abiotic stress responses journal April 2010
Data Processing for GC-MS- and LC-MS-Based Untargeted Metabolomics book January 2019
Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response to Botrytis infection journal October 2006
Life cycle assessment of different bioenergy production systems including perennial and annual crops journal December 2011
Gas chromatographic–mass spectrometric investigation of metabolites from the needles and roots of pine seedlings at early stages of pathogenic fungi Armillaria ostoyae attack journal February 2008
Whole-genome sequencing reveals untapped genetic potential in Africa’s indigenous cereal crop sorghum journal August 2013
Prephenate aminotransferase directs plant phenylalanine biosynthesis via arogenate journal November 2010
The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems journal March 2008
Significance of Inducible Defense-related Proteins in Infected Plants journal September 2006

Similar Records

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
metabolism
metabolomics
microbiome
nitrogen
rhizosphere
roots
salicylic acid
sorghum
stress