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Title: Microfluidic Sensors with Impregnated Fluorophores for Simultaneous Imaging of Spatial Structure and Chemical Oxygen Gradients

Abstract

Inside surfaces of polystyrene microfluidic structures were impregnated with the oxygen sensing dye (PtTFPP) using a solvent-induced fluorophore impregnation (SIFI) method. By this technique, microfluidic oxygen sensors are obtained that enable simultaneous imaging of both chemical oxygen gradients and the physical structure of the microfluidic interior. A gentle method of fluorophore impregnation using acetonitrile solutions of PtTFPP at 50oC was developed leading to 10 micron deep region containing fluorophore. This region is localized at the surface to sense oxygen in the interior fluid during use. Regions of the device that do not contact the interior fluid pathways lack fluorophores and are dark in fluorescent imaging. The technique was demonstrated on straight microchannel and pore network devices, the latter having pillars of 300 µm diameter spaced center to center at 340 µm providing pore throats of 40 µm. Sensing within channels or pores, and imaging across the pore network devices were performed using a Lambert LIFA-P frequency domain fluorescence lifetime imaging system on a Leical microscope platform. Calibrations of different devices prepared by the SIFI method were indistinguishable. Gradient imaging showed fluorescent regions corresponding to the fluid pore network, dark pillars, and fluorescent lifetime varying across the gradient, thus providing bothmore » physical and chemical imaging. More generally, the SIFI technique can impregnate the interior surfaces of other polystyrene containers, such as cuvettes or cell and tissue culture containers, to enable sensing interior conditions.« less

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [2]
  1. Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, United States
  2. Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan 48109, United States
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1506985
Report Number(s):
PNNL-SA-137290
Journal ID: ISSN 2379-3694
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
ACS Sensors
Additional Journal Information:
Journal Volume: 4; Journal Issue: 2; Journal ID: ISSN 2379-3694
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
microfluidic, sensor, imaging, oxygen

Citation Formats

Grate, Jay W., Liu, Bingwen, Kelly, Ryan T., Anheier, Norman C., and Schmidt, Thomas M. Microfluidic Sensors with Impregnated Fluorophores for Simultaneous Imaging of Spatial Structure and Chemical Oxygen Gradients. United States: N. p., 2019. Web. doi:10.1021/acssensors.8b00924.
Grate, Jay W., Liu, Bingwen, Kelly, Ryan T., Anheier, Norman C., & Schmidt, Thomas M. Microfluidic Sensors with Impregnated Fluorophores for Simultaneous Imaging of Spatial Structure and Chemical Oxygen Gradients. United States. doi:10.1021/acssensors.8b00924.
Grate, Jay W., Liu, Bingwen, Kelly, Ryan T., Anheier, Norman C., and Schmidt, Thomas M. Fri . "Microfluidic Sensors with Impregnated Fluorophores for Simultaneous Imaging of Spatial Structure and Chemical Oxygen Gradients". United States. doi:10.1021/acssensors.8b00924.
@article{osti_1506985,
title = {Microfluidic Sensors with Impregnated Fluorophores for Simultaneous Imaging of Spatial Structure and Chemical Oxygen Gradients},
author = {Grate, Jay W. and Liu, Bingwen and Kelly, Ryan T. and Anheier, Norman C. and Schmidt, Thomas M.},
abstractNote = {Inside surfaces of polystyrene microfluidic structures were impregnated with the oxygen sensing dye (PtTFPP) using a solvent-induced fluorophore impregnation (SIFI) method. By this technique, microfluidic oxygen sensors are obtained that enable simultaneous imaging of both chemical oxygen gradients and the physical structure of the microfluidic interior. A gentle method of fluorophore impregnation using acetonitrile solutions of PtTFPP at 50oC was developed leading to 10 micron deep region containing fluorophore. This region is localized at the surface to sense oxygen in the interior fluid during use. Regions of the device that do not contact the interior fluid pathways lack fluorophores and are dark in fluorescent imaging. The technique was demonstrated on straight microchannel and pore network devices, the latter having pillars of 300 µm diameter spaced center to center at 340 µm providing pore throats of 40 µm. Sensing within channels or pores, and imaging across the pore network devices were performed using a Lambert LIFA-P frequency domain fluorescence lifetime imaging system on a Leical microscope platform. Calibrations of different devices prepared by the SIFI method were indistinguishable. Gradient imaging showed fluorescent regions corresponding to the fluid pore network, dark pillars, and fluorescent lifetime varying across the gradient, thus providing both physical and chemical imaging. More generally, the SIFI technique can impregnate the interior surfaces of other polystyrene containers, such as cuvettes or cell and tissue culture containers, to enable sensing interior conditions.},
doi = {10.1021/acssensors.8b00924},
journal = {ACS Sensors},
issn = {2379-3694},
number = 2,
volume = 4,
place = {United States},
year = {2019},
month = {1}
}