CAD 1 and CCR 2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

Yan, Xiaojing; Liu, Jie; Kim, Hoon; Liu, Baoguang; Huang, Xiong; Yang, Zhichang; Lin, Ying‐Chung Jimmy; Chen, Hao; Yang, Chenmin; Wang, Jack P.; Muddiman, David C.; Ralph, John; Sederoff, Ronald R.; Li, Quanzi; Chiang, Vincent L.

doi:10.1111/nph.15505

Title: CAD 1 and CCR 2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

Journal Article · Mon Oct 01 00:00:00 EDT 2018 · New Phytologist

DOI:https://doi.org/10.1111/nph.15505· OSTI ID:1506674

Yan, Xiaojing ^[1]; Liu, Jie ^[2]; Kim, Hoon ^[3]; Liu, Baoguang ^[4]; Huang, Xiong ^[1]; Yang, Zhichang ^[2]; Lin, Ying‐Chung Jimmy ^[5]; Chen, Hao ^[2]; Yang, Chenmin ^[2]; Wang, Jack P. ^[6]; Muddiman, David C. ^[2]; Ralph, John ^[3]; Sederoff, Ronald R. ^[2]; Li, Quanzi ^[1]; Chiang, Vincent L. ^[6]

Chinese Academy of Forestry, Beijing (China)
North Carolina State Univ., Raleigh, NC (United States)
Univ. of Wisconsin, Madison, WI (United States)
Northeast Forestry Univ., Harbin (China); Beihua Univ., Jilin (China)
Northeast Forestry Univ., Harbin (China); National Taiwan Univ., Taipei (Taiwan)
North Carolina State Univ., Raleigh, NC (United States); Northeast Forestry Univ., Harbin (China)

Summary Lignin is the major phenolic polymer in plant secondary cell walls and is polymerized from monomeric subunits, the monolignols. Eleven enzyme families are implicated in monolignol biosynthesis. Here, we studied the functions of members of the cinnamyl alcohol dehydrogenase ( CAD ) and cinnamoyl‐CoA reductase ( CCR ) families in wood formation in Populus trichocarpa , including the regulatory effects of their transcripts and protein activities on monolignol biosynthesis. Enzyme activity assays from stem‐differentiating xylem ( SDX ) proteins showed that RNA i suppression of Ptr CAD 1 in P. trichocarpa transgenics caused a reduction in SDX CCR activity. RNA i suppression of Ptr CCR 2 , the only CCR member highly expressed in SDX , caused a reciprocal reduction in SDX protein CAD activities. The enzyme assays of mixed and coexpressed recombinant proteins supported physical interactions between Ptr CAD 1 and Ptr CCR 2. Biomolecular fluorescence complementation and pull‐down/co‐immunoprecipitation experiments supported a hypothesis of Ptr CAD 1/Ptr CCR 2 heterodimer formation. These results provide evidence for the formation of Ptr CAD 1/Ptr CCR 2 protein complexes in monolignol biosynthesis in planta .

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409; BER DE‐FC02‐07ER64494; DE‐SC0018409

OSTI ID:: 1506674

Alternate ID(s):: OSTI ID: 1479510

Journal Information:: New Phytologist, Vol. 222, Issue 1; ISSN 0028-646X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 29 works

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59 BASIC BIOLOGICAL SCIENCES
co‐immunoprecipitation
enzyme activity
monolignol biosynthetic pathway
nuclear magnetic resonance (NMR)
Populus trichocarpa
stem‐differentiating xylem protein

Title: CAD 1 and CCR 2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

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