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Title: Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production

Abstract

Sorghum (Sorghum bicolor L. Moench) offers substantial potential as a feedstock for the production of sugar-derived biofuels and biochemical products from cell wall polysaccharides (i. e., cellulose and hemicelluloses) and water-extractable sugars (i.e., glucose, fructose, sucrose, and starch). A number of preprocessing schemes can be envisioned that involve processes such as sugar extraction, pretreatment, and densification that could be employed in decentralized, regional-scale biomass processing depots. In this work, an energy sorghum exhibiting a combination of high biomass productivity and high sugar accumulation was evaluated for its potential for integration into several potential biomass preprocessing schemes. This included counter-current extraction of water-soluble sugars followed by mild NaOH or liquid hot water pretreatment of the extracted bagasse. A novel processing scheme was investigated that could integrate with current diffuser-type extraction systems for sugar extraction. In this approach, mild NaOH pretreatment (i.e., <90°C) was performed as a counter-current extraction to yield both an extracted, pretreated bagasse and a high-concentration mixed sugar stream. Following hydrolysis of the bagasse, the combined hydrolysates derived from cellulosic sugars and extractable sugars were demonstrated to be fermentable to high ethanol titers (>8%) at high metabolic yields without detoxification using a Saccharomyces cerevisiae strain metabolically engineered and evolvedmore » to ferment xylose.« less

Authors:
 [1];  [2];  [3];  [4]
  1. Michigan State Univ., East Lansing, MI (United States); Michigan State University, Madison, MI (United States)
  2. Michigan State University, Madison, MI (United States); Michigan Technological University, Houghton, MI (United States)
  3. Michigan State University, Madison, MI (United States); Texas A & M Univ., College Station, TX (United States)
  4. Montana State Univ., Bozeman, MT (United States); Luleå University of Technology (Sweden)
Publication Date:
Research Org.:
Great Lakes Bioenergy Research Center, Madison, MI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1506483
Grant/Contract Number:  
SC0018409; FC02-07ER64494
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Frontiers in Energy Research
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2296-598X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Sorghum; sucrose extraction; decentralized biorefining; pretreatment; cellulosic biofuels

Citation Formats

Williams, Daniel L., Ong, Rebecca G., Mullet, John E., and Hodge, David B.. Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production. United States: N. p., 2019. Web. doi:10.3389/fenrg.2018.00133.
Williams, Daniel L., Ong, Rebecca G., Mullet, John E., & Hodge, David B.. Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production. United States. doi:10.3389/fenrg.2018.00133.
Williams, Daniel L., Ong, Rebecca G., Mullet, John E., and Hodge, David B.. Mon . "Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production". United States. doi:10.3389/fenrg.2018.00133. https://www.osti.gov/servlets/purl/1506483.
@article{osti_1506483,
title = {Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production},
author = {Williams, Daniel L. and Ong, Rebecca G. and Mullet, John E. and Hodge, David B.},
abstractNote = {Sorghum (Sorghum bicolor L. Moench) offers substantial potential as a feedstock for the production of sugar-derived biofuels and biochemical products from cell wall polysaccharides (i. e., cellulose and hemicelluloses) and water-extractable sugars (i.e., glucose, fructose, sucrose, and starch). A number of preprocessing schemes can be envisioned that involve processes such as sugar extraction, pretreatment, and densification that could be employed in decentralized, regional-scale biomass processing depots. In this work, an energy sorghum exhibiting a combination of high biomass productivity and high sugar accumulation was evaluated for its potential for integration into several potential biomass preprocessing schemes. This included counter-current extraction of water-soluble sugars followed by mild NaOH or liquid hot water pretreatment of the extracted bagasse. A novel processing scheme was investigated that could integrate with current diffuser-type extraction systems for sugar extraction. In this approach, mild NaOH pretreatment (i.e., <90°C) was performed as a counter-current extraction to yield both an extracted, pretreated bagasse and a high-concentration mixed sugar stream. Following hydrolysis of the bagasse, the combined hydrolysates derived from cellulosic sugars and extractable sugars were demonstrated to be fermentable to high ethanol titers (>8%) at high metabolic yields without detoxification using a Saccharomyces cerevisiae strain metabolically engineered and evolved to ferment xylose.},
doi = {10.3389/fenrg.2018.00133},
journal = {Frontiers in Energy Research},
issn = {2296-598X},
number = ,
volume = 6,
place = {United States},
year = {2019},
month = {1}
}

Journal Article:
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Works referenced in this record:

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book, January 1999

  • Lee, Y. Y.; Iyer, Prashant; Torget, R. W.
  • Recent Progress in Bioconversion of Lignocellulosics, p. 93-115
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Alkaline Pretreatment of Corn Stover: Bench-Scale Fractionation and Stream Characterization
journal, May 2014

  • Karp, Eric M.; Donohoe, Bryon S.; O�Brien, Marykate H.
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  • DOI: 10.1021/sc500126u

Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition
journal, August 2000


Optimization of pH controlled liquid hot water pretreatment of corn stover
journal, December 2005