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Title: A database-driven approach identifies additional diterpene synthase activities in the mint family (Lamiaceae)

Abstract

Members of the mint family (Lamiaceae) accumulate a wide variety of industrially and medicinally relevant diterpenes. We recently sequenced leaf transcriptomes from 48 phylogenetically diverse Lamiaceae species. Here, we summarize the available chemotaxonomic and enzyme activity data for diterpene synthases (diTPSs) in the Lamiaceae and leverage the new transcriptomes to explore the diTPS sequence and functional space. Candidate genes were selected with an intent to evenly sample the sequence homology space and to focus on species in which diTPS transcripts were found, yet from which no diterpene structures have been previously reported. We functionally characterized nine class II diTPSs and 10 class I diTPSs from 11 distinct plant species and found five class II activities, including two novel activities, as well as a spectrum of class I activities. Among the class II diTPSs, we identified a neo-cleroda-4(18),13E-dienyl diphosphate synthase from Ajuga reptans, catalyzing the likely first step in the biosynthesis of a variety of insect-antifeedant compounds. Among the class I diTPSs was a palustradiene synthase from Origanum majorana, leading to the discovery of specialized diterpenes in that species. Our results provide insights into the diversification of diterpene biosynthesis in the mint family and establish a comprehensive foundation for continued investigationmore » of diterpene biosynthesis in the Lamiaceae.« less

Authors:
 [1]; ORCiD logo [1]; ORCiD logo [1];  [1];  [1]; ORCiD logo [1]
  1. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Research Org.:
Great Lakes Bioenergy Research Center, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Contributing Org.:
Evolutionary Mint Genomics Consortium
OSTI Identifier:
1506454
Grant/Contract Number:  
SC0018409
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Volume: 294; Journal Issue: 4; Journal ID: ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; secondary metabolism; chemotaxonomy; terpenoid; plant biochemistry; transcriptomics; biosynthesis; enzymes; diterpene synthase; Lamiaceae; phytochemistry

Citation Formats

Johnson, Sean R., Bhat, Wajid Waheed, Bibik, Jacob, Turmo, Aiko, Hamberger, Britta, and Hamberger, Björn. A database-driven approach identifies additional diterpene synthase activities in the mint family (Lamiaceae). United States: N. p., 2018. Web. doi:10.1074/jbc.RA118.006025.
Johnson, Sean R., Bhat, Wajid Waheed, Bibik, Jacob, Turmo, Aiko, Hamberger, Britta, & Hamberger, Björn. A database-driven approach identifies additional diterpene synthase activities in the mint family (Lamiaceae). United States. doi:10.1074/jbc.RA118.006025.
Johnson, Sean R., Bhat, Wajid Waheed, Bibik, Jacob, Turmo, Aiko, Hamberger, Britta, and Hamberger, Björn. Thu . "A database-driven approach identifies additional diterpene synthase activities in the mint family (Lamiaceae)". United States. doi:10.1074/jbc.RA118.006025. https://www.osti.gov/servlets/purl/1506454.
@article{osti_1506454,
title = {A database-driven approach identifies additional diterpene synthase activities in the mint family (Lamiaceae)},
author = {Johnson, Sean R. and Bhat, Wajid Waheed and Bibik, Jacob and Turmo, Aiko and Hamberger, Britta and Hamberger, Björn},
abstractNote = {Members of the mint family (Lamiaceae) accumulate a wide variety of industrially and medicinally relevant diterpenes. We recently sequenced leaf transcriptomes from 48 phylogenetically diverse Lamiaceae species. Here, we summarize the available chemotaxonomic and enzyme activity data for diterpene synthases (diTPSs) in the Lamiaceae and leverage the new transcriptomes to explore the diTPS sequence and functional space. Candidate genes were selected with an intent to evenly sample the sequence homology space and to focus on species in which diTPS transcripts were found, yet from which no diterpene structures have been previously reported. We functionally characterized nine class II diTPSs and 10 class I diTPSs from 11 distinct plant species and found five class II activities, including two novel activities, as well as a spectrum of class I activities. Among the class II diTPSs, we identified a neo-cleroda-4(18),13E-dienyl diphosphate synthase from Ajuga reptans, catalyzing the likely first step in the biosynthesis of a variety of insect-antifeedant compounds. Among the class I diTPSs was a palustradiene synthase from Origanum majorana, leading to the discovery of specialized diterpenes in that species. Our results provide insights into the diversification of diterpene biosynthesis in the mint family and establish a comprehensive foundation for continued investigation of diterpene biosynthesis in the Lamiaceae.},
doi = {10.1074/jbc.RA118.006025},
journal = {Journal of Biological Chemistry},
issn = {0021-9258},
number = 4,
volume = 294,
place = {United States},
year = {2018},
month = {11}
}

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