A database-driven approach identifies additional diterpene synthase activities in the mint family (Lamiaceae)

Johnson, Sean R.; Bhat, Wajid Waheed; Bibik, Jacob; Turmo, Aiko; Hamberger, Britta; Evolutionary Mint Genomics Consortium,; Hamberger, Björn

doi:10.1074/jbc.RA118.006025

Title: A database-driven approach identifies additional diterpene synthase activities in the mint family (Lamiaceae)

Journal Article · Tue Jan 01 00:00:00 EST 2019 · Journal of Biological Chemistry

DOI:https://doi.org/10.1074/jbc.RA118.006025· OSTI ID:1769439

Johnson, Sean R.;

;

; Turmo, Aiko; Hamberger, Britta; Evolutionary Mint Genomics Consortium,;

Members of the mint family (Lamiaceae) accumulate a wide variety of industrially and medicinally relevant diterpenes. We recently sequenced leaf transcriptomes from 48 phylogenetically diverse Lamiaceae species. Here, we summarize the available chemotaxonomic and enzyme activity data for diterpene synthases (diTPSs) in the Lamiaceae and leverage the new transcriptomes to explore the diTPS sequence and functional space. Candidate genes were selected with an intent to evenly sample the sequence homology space and to focus on species in which diTPS transcripts were found, yet from which no diterpene structures have been previously reported. We functionally characterized nine class II diTPSs and 10 class I diTPSs from 11 distinct plant species and found five class II activities, including two novel activities, as well as a spectrum of class I activities. Among the class II diTPSs, we identified a neo-cleroda-4(18),13E-dienyl diphosphate synthase from Ajuga reptans, catalyzing the likely first step in the biosynthesis of a variety of insect-antifeedant compounds. Among the class I diTPSs was a palustradiene synthase from Origanum majorana, leading to the discovery of specialized diterpenes in that species. Our results provide insights into the diversification of diterpene biosynthesis in the mint family and establish a comprehensive foundation for continued investigation of diterpene biosynthesis in the Lamiaceae.

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Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Organization:: Evolutionary Mint Genomics Consortium

Grant/Contract Number:: SC0018409

OSTI ID:: 1769439

Alternate ID(s):: OSTI ID: 1506454

Journal Information:: Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Vol. 294 Journal Issue: 4; ISSN 0021-9258

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 31 works

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The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway Helliwell, C. A. Proceedings of the National Academy of Sciences, Vol. 98, Issue 4 https://doi.org/10.1073/pnas.041588998	journal	February 2001
Functional divergence of diterpene syntheses in the medicinal plant Salvia miltiorrhiza Bunge Cui, Guanghong; Duan, Lixin; Jin, Baolong Plant Physiology https://doi.org/10.1104/pp.15.00695	journal	June 2015

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Conserved bases for the initial cyclase in gibberellin biosynthesis: from bacteria to plants Lemke, Cody; Potter, Kevin C.; Schulte, Samuel Biochemical Journal, Vol. 476, Issue 18 https://doi.org/10.1042/bcj20190479	journal	September 2019
Promiscuous terpene synthases from Prunella vulgaris highlight the importance of substrate and compartment switching in terpene synthase evolution Johnson, Sean R.; Bhat, Wajid Waheed; Sadre, Radin New Phytologist, Vol. 223, Issue 1 https://doi.org/10.1111/nph.15778	journal	April 2019
Computer-Aided Chemotaxonomy and Bioprospecting Study of Diterpenes of the Lamiaceae Family Barbosa Silva Cavalcanti, Andreza; Costa Barros, Renata Priscila; Costa, Vicente Carlos de Oliveira Molecules, Vol. 24, Issue 21 https://doi.org/10.3390/molecules24213908	journal	October 2019

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