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Title: Immobilized purple bacteria for light-driven H2 production from starch and potato fermentation effluents

Abstract

The goal of the study was to show that immobilized purple bacteria could photoproduce H2 using dark fermentation effluent (FE) as substrate. Simple pretreatment of an inexpensive glass-fiber matrix accelerated the immobilization process. Photobioreactors (PhBR) containing immobilized Rhodobacter sphaeroides GL produced 0.128 L H2 h-1 L-1 of PhBR volume (0.570 L h-1 L-1 of matrix) for upto 3 months when continuously fed artificial media with volatile fatty acids (VFAs) or FE from potato and starch fermentations. Hydrogen production was insensitive to NH4+ up to 1 mM and saturated at 8 mM lactate or 1.5% potato FE (diluted in water and supplemented with critical micronutrients). The efficiency of VFA transformation to H2 was 50-70% of theoretical. At nonlimiting substrateconcentrations in artificial media or FE, acetate was utilized before butyrate. High volumetric rates of continuous H2 photoproduction and stability of the process are advantages of using immobilized cultures. Use of H2 photoproduction as a polishing step in the treatment of FEs from dark fermentations increased the total amount of H2 produced from 0.9 to 4.7 mol mol-1 glucose equivalent inthe original potato homogenate.

Authors:
; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Fuel Cell Technologies Office (EE-3F)
OSTI Identifier:
1505924
Report Number(s):
NREL/JA-2A00-51376
Journal ID: ISSN 8756-7938
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article
Journal Name:
Biotechnology Progress
Additional Journal Information:
Journal Volume: 27; Journal Issue: 5; Journal ID: ISSN 8756-7938
Publisher:
Society for Biological Engineering
Country of Publication:
United States
Language:
English
Subject:
08 HYDROGEN; hydrogen production; photosynthetic bacteria; integrated system; potato fermentation; immobilization

Citation Formats

Tekucheva, Darya N., Laurinavichene, Tatyana V., Seibert, Michael, and Tsygankov, Anatoly A.. Immobilized purple bacteria for light-driven H2 production from starch and potato fermentation effluents. United States: N. p., 2011. Web. doi:10.1002/btpr.668.
Tekucheva, Darya N., Laurinavichene, Tatyana V., Seibert, Michael, & Tsygankov, Anatoly A.. Immobilized purple bacteria for light-driven H2 production from starch and potato fermentation effluents. United States. doi:10.1002/btpr.668.
Tekucheva, Darya N., Laurinavichene, Tatyana V., Seibert, Michael, and Tsygankov, Anatoly A.. Tue . "Immobilized purple bacteria for light-driven H2 production from starch and potato fermentation effluents". United States. doi:10.1002/btpr.668.
@article{osti_1505924,
title = {Immobilized purple bacteria for light-driven H2 production from starch and potato fermentation effluents},
author = {Tekucheva, Darya N. and Laurinavichene, Tatyana V. and Seibert, Michael and Tsygankov, Anatoly A.},
abstractNote = {The goal of the study was to show that immobilized purple bacteria could photoproduce H2 using dark fermentation effluent (FE) as substrate. Simple pretreatment of an inexpensive glass-fiber matrix accelerated the immobilization process. Photobioreactors (PhBR) containing immobilized Rhodobacter sphaeroides GL produced 0.128 L H2 h-1 L-1 of PhBR volume (0.570 L h-1 L-1 of matrix) for upto 3 months when continuously fed artificial media with volatile fatty acids (VFAs) or FE from potato and starch fermentations. Hydrogen production was insensitive to NH4+ up to 1 mM and saturated at 8 mM lactate or 1.5% potato FE (diluted in water and supplemented with critical micronutrients). The efficiency of VFA transformation to H2 was 50-70% of theoretical. At nonlimiting substrateconcentrations in artificial media or FE, acetate was utilized before butyrate. High volumetric rates of continuous H2 photoproduction and stability of the process are advantages of using immobilized cultures. Use of H2 photoproduction as a polishing step in the treatment of FEs from dark fermentations increased the total amount of H2 produced from 0.9 to 4.7 mol mol-1 glucose equivalent inthe original potato homogenate.},
doi = {10.1002/btpr.668},
journal = {Biotechnology Progress},
issn = {8756-7938},
number = 5,
volume = 27,
place = {United States},
year = {2011},
month = {7}
}