A two-helix motif positions the lysophosphatidic acid acyltransferase active site for catalysis within the membrane bilayer

doi:10.1038/nsmb.3436

Title: A two-helix motif positions the lysophosphatidic acid acyltransferase active site for catalysis within the membrane bilayer

Authors:

Robertson, Rosanna M.;

; Gajewski, Stefan;

; Martin, Erik W.;

;

Publication Date:: 2017-07-17

Research Org.:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Org.:: NCINIHOTHER

OSTI Identifier:: 1504990

Resource Type:: Journal Article

Journal Name:: Nature Structural & Molecular Biology

Additional Journal Information:: Journal Volume: 24; Journal Issue: 8; Journal ID: ISSN 1545-9993

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: ENGLISH

Citation Formats


                    Robertson, Rosanna M., Yao, Jiangwei, Gajewski, Stefan, Kumar, Gyanendra, Martin, Erik W., Rock, Charles O., and White, Stephen W. A two-helix motif positions the lysophosphatidic acid acyltransferase active site for catalysis within the membrane bilayer.  United States: N. p., 2017. 
        Web.  doi:10.1038/nsmb.3436.

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                    Robertson, Rosanna M., Yao, Jiangwei, Gajewski, Stefan, Kumar, Gyanendra, Martin, Erik W., Rock, Charles O., & White, Stephen W. A two-helix motif positions the lysophosphatidic acid acyltransferase active site for catalysis within the membrane bilayer.  United States.  doi:10.1038/nsmb.3436.

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                    Robertson, Rosanna M., Yao, Jiangwei, Gajewski, Stefan, Kumar, Gyanendra, Martin, Erik W., Rock, Charles O., and White, Stephen W. Mon .  
        "A two-helix motif positions the lysophosphatidic acid acyltransferase active site for catalysis within the membrane bilayer".  United States.  doi:10.1038/nsmb.3436.

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@article{osti_1504990,

  title        = {A two-helix motif positions the lysophosphatidic acid acyltransferase active site for catalysis within the membrane bilayer},

  author       = {Robertson, Rosanna M. and Yao, Jiangwei and Gajewski, Stefan and Kumar, Gyanendra and Martin, Erik W. and Rock, Charles O. and White, Stephen W.},

  abstractNote = {},

  doi          = {10.1038/nsmb.3436},

  journal      = {Nature Structural & Molecular Biology},

  issn         = {1545-9993},

  number       = 8,

  volume       = 24,

  place        = {United States},

  year         = {2017},

  month        = {7}

}

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DOI: 10.1038/nsmb.3436

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Title: A two-helix motif positions the lysophosphatidic acid acyltransferase active site for catalysis within the membrane bilayer

Citation Formats

Membrane topology of human AGPAT3 (LPAAT3) journal, July 2010

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Analysis of Amino Acid Motifs Diagnostic for the sn -Glycerol-3-phosphate Acyltransferase Reaction † journal, May 1999

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Structure and mechanism of an intramembrane liponucleotide synthetase central for phospholipid biosynthesis journal, June 2014

Crystal structure of LpxK, the 4'-kinase of lipid A biosynthesis and atypical P-loop kinase functioning at the membrane interface journal, July 2012

Phosphatidic acid synthesis in bacteria journal, March 2013

Generation of membrane diversity by lysophospholipid acyltransferases journal, May 2013

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Maximum-likelihood density modification journal, August 2000

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Substrate recognition and selectivity of plant glycerol-3-phosphate acyltransferases (GPATs) from Cucurbita moscata and Spinacea oleracea journal, December 2003

Analysis of the Structure, Substrate Specificity, and Mechanism of Squash Glycerol-3-Phosphate (1)-Acyltransferase journal, May 2001

Acyl-CoA:Lysophospholipid Acyltransferases journal, August 2008

Topology of acyltransferase motifs and substrate specificity and accessibility in 1-acyl-sn-glycero-3-phosphate acyltransferase 1 journal, September 2007

Acylation of glycerol 3-phosphate is the sole pathway of de novo phospholipid synthesis in Escherichia coli. journal, June 1987

Acyltransferases and transacylases that determine the fatty acid composition of glycerolipids and the metabolism of bioactive lipid mediators in mammalian cells and model organisms journal, January 2014

A Rapid Method of Total Lipid Extraction and Purification journal, January 1959

Experimental phasing with SHELXC / D / E : combining chain tracing with density modification journal, March 2010

Efficient solvent boundary potential for hybrid potential simulations journal, January 2011

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Lysophospholipid acyltransferases: 1-acylglycerol-3-phosphate O-acyltransferases. From discovery to disease journal, January 2012

Phaser crystallographic software journal, July 2007

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Chasing acyl carrier protein through a catalytic cycle of lipid A production
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Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard
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The Structural Biology of type ii Fatty acid Biosynthesis
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journal, June 2013

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journal, May 2009

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AGPAT2 is mutated in congenital generalized lipodystrophy linked to chromosome 9q34
journal, April 2002

Crystallization and preliminary X-ray analysis of the glycerol-3-phosphate 1-acyltransferase from squash ( Cucurbita moschata )
journal, March 2001

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journal, January 2002

Automation of the CHARMM General Force Field (CGenFF) II: Assignment of Bonded Parameters and Partial Atomic Charges
journal, November 2012

Update of the CHARMM All-Atom Additive Force Field for Lipids: Validation on Six Lipid Types
journal, June 2010

XDS
journal, January 2010

Evidence toward a Dual Phosphatase Mechanism That Restricts Aurora A (Thr-295) Phosphorylation during the Early Embryonic Cell Cycle
journal, May 2014

Analysis of Amino Acid Motifs Diagnostic for the sn -Glycerol-3-phosphate Acyltransferase Reaction ^†
journal, May 1999

Crystal Structure of Human Cytosolic Phospholipase A2 Reveals a Novel Topology and Catalytic Mechanism
journal, April 1999

Structure and mechanism of an intramembrane liponucleotide synthetase central for phospholipid biosynthesis
journal, June 2014

Crystal structure of LpxK, the 4'-kinase of lipid A biosynthesis and atypical P-loop kinase functioning at the membrane interface
journal, July 2012

Phosphatidic acid synthesis in bacteria
journal, March 2013

Generation of membrane diversity by lysophospholipid acyltransferases
journal, May 2013

Membrane-protein topology
journal, December 2006

Trapping the dynamic acyl carrier protein in fatty acid biosynthesis
journal, December 2013

Maximum-likelihood density modification
journal, August 2000

Features and development of Coot
journal, March 2010

Analysis of the Staphylococcus aureus DgkB Structure Reveals a Common Catalytic Mechanism for the Soluble Diacylglycerol Kinases
journal, July 2008

Substrate recognition and selectivity of plant glycerol-3-phosphate acyltransferases (GPATs) from Cucurbita moscata and Spinacea oleracea
journal, December 2003

Analysis of the Structure, Substrate Specificity, and Mechanism of Squash Glycerol-3-Phosphate (1)-Acyltransferase
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Acyl-CoA:Lysophospholipid Acyltransferases
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Topology of acyltransferase motifs and substrate specificity and accessibility in 1-acyl-sn-glycero-3-phosphate acyltransferase 1
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Acylation of glycerol 3-phosphate is the sole pathway of de novo phospholipid synthesis in Escherichia coli.
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Acyltransferases and transacylases that determine the fatty acid composition of glycerolipids and the metabolism of bioactive lipid mediators in mammalian cells and model organisms
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Experimental phasing with SHELXC / D / E : combining chain tracing with density modification
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Efficient solvent boundary potential for hybrid potential simulations
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Molecular structures of fluid phase phosphatidylglycerol bilayers as determined by small angle neutron and X-ray scattering
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Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA
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