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Title: Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility

Abstract

The high cost of enzymes is one of the key technical barriers that must be overcome to realize the economical production of biofuels and biomaterials from biomass. Supplementation of enzyme cocktails with lytic polysaccharide monooxygenase (LPMO) can increase the efficiency of these cellulase mixtures for biomass conversion. The previous studies have revealed that LPMOs cleave polysaccharide chains by oxidization of the C1 and/or C4 carbons of the monomeric units. However, how LPMOs enhance enzymatic degradation of lignocellulose is still poorly understood.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [1]; ORCiD logo [1]
  1. Michigan State Univ., East Lansing, MI (United States)
  2. South Dakota School of Mines and Technology, Rapid City, SD (United States)
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States); Harvard Univ., Cambridge, MA (United States
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1504758
Grant/Contract Number:  
FC02-07ER64494; SC0012411; DMR-BMART-1206908; FG02-91ER200021
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; LPMO; CBH I; AFM; Lignocellulose; Biomass; Cellulose; Biorefinery

Citation Formats

Song, Bo, Li, Bingyao, Wang, Xiaoyan, Shen, Wei, Park, Sungjin, Collings, Cynthia, Feng, Anran, Smith, Steve J., Walton, Jonathan D., and Ding, Shi -You. Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility. United States: N. p., 2018. Web. doi:10.1186/s13068-018-1023-1.
Song, Bo, Li, Bingyao, Wang, Xiaoyan, Shen, Wei, Park, Sungjin, Collings, Cynthia, Feng, Anran, Smith, Steve J., Walton, Jonathan D., & Ding, Shi -You. Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility. United States. doi:10.1186/s13068-018-1023-1.
Song, Bo, Li, Bingyao, Wang, Xiaoyan, Shen, Wei, Park, Sungjin, Collings, Cynthia, Feng, Anran, Smith, Steve J., Walton, Jonathan D., and Ding, Shi -You. Thu . "Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility". United States. doi:10.1186/s13068-018-1023-1. https://www.osti.gov/servlets/purl/1504758.
@article{osti_1504758,
title = {Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility},
author = {Song, Bo and Li, Bingyao and Wang, Xiaoyan and Shen, Wei and Park, Sungjin and Collings, Cynthia and Feng, Anran and Smith, Steve J. and Walton, Jonathan D. and Ding, Shi -You},
abstractNote = {The high cost of enzymes is one of the key technical barriers that must be overcome to realize the economical production of biofuels and biomaterials from biomass. Supplementation of enzyme cocktails with lytic polysaccharide monooxygenase (LPMO) can increase the efficiency of these cellulase mixtures for biomass conversion. The previous studies have revealed that LPMOs cleave polysaccharide chains by oxidization of the C1 and/or C4 carbons of the monomeric units. However, how LPMOs enhance enzymatic degradation of lignocellulose is still poorly understood.},
doi = {10.1186/s13068-018-1023-1},
journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 1,
volume = 11,
place = {United States},
year = {2018},
month = {2}
}

Journal Article:
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